Molecules,
Год журнала:
2024,
Номер
29(21), С. 5023 - 5023
Опубликована: Окт. 24, 2024
The
deposition
of
amyloid
β-protein
(Aβ)
in
the
brain
is
main
pathogenesis
Alzheimer's
disease
(AD).
development
potent
inhibitors
against
Aβ
aggregation
one
effective
strategies
to
combat
AD.
Endogenous
transthyretin
(TTR)
can
inhibit
fibrillization
via
hydrophobic
interactions,
but
its
weak
inhibitory
potency
hinders
application
AD
therapy.
Here,
different
recombinant
TTRs
were
designed
by
cationic
surface
charge
engineering.
Compared
with
TTR,
all
positively
charged
showed
enhanced
capability
inhibiting
aggregation,
especially
protein
obtained
mutating
acidic
amino
acid
TTR
arginine
(TTR-nR)
exhibited
excellent
effect.
Among
them,
TTR-7R
remarkably
increased
Aβ,
which
could
effectively
Microorganisms,
Год журнала:
2025,
Номер
13(3), С. 566 - 566
Опубликована: Март 2, 2025
The
global
nutraceutical
industry
is
experiencing
a
paradigm
shift,
driven
by
an
increasing
demand
for
functional
foods
and
dietary
supplements
that
address
malnutrition
chronic
diseases
such
as
obesity,
diabetes,
cardiovascular
conditions,
cancer.
Traditional
plant-
animal-derived
nutraceuticals
face
limitations
in
scalability,
cost,
environmental
impact,
paving
the
way
microbial
biotechnology
sustainable
alternative.
Microbial
cells
act
bio-factories,
converting
nutrients
like
glucose
amino
acids
into
valuable
products
polyunsaturated
fatty
(PUFAs),
peptides,
other
bioactive
compounds.
By
harnessing
their
natural
metabolic
capabilities,
microorganisms
efficiently
synthesize
these
compounds,
making
production
effective
approach
development.
This
review
explores
transformative
role
of
platforms
nutraceuticals,
emphasizing
advanced
fermentation
techniques,
synthetic
biology,
engineering.
It
addresses
challenges
optimizing
strains,
ensuring
product
quality,
scaling
while
navigating
regulatory
frameworks.
Furthermore,
highlights
cutting-edge
technologies
CRISPR/Cas9
genome
editing,
adaptive
evolution
strain
enhancement,
bioreactor
innovations
to
enhance
yield
efficiency.
With
focus
on
sustainability
precision,
positioned
game-changer
industry,
offering
eco-friendly
scalable
solutions
meet
health
needs.
integration
omics
exploration
novel
sources
hold
potential
revolutionize
this
field,
aligning
with
growing
consumer
innovative
products.
Abstract
Microbial
products
are
essential
for
developing
various
therapeutic
agents,
including
antibiotics,
anticancer
drugs,
vaccines,
and
enzymes.
Genetic
engineering
techniques,
functional
genomics,
synthetic
biology
unlock
previously
uncharacterized
natural
products.
This
review
highlights
major
advances
in
microbial
biotechnology,
focusing
on
gene‐based
technologies
medical
applications.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(14), С. 7603 - 7603
Опубликована: Июль 11, 2024
This
article
presents
a
groundbreaking
perspective
on
carotenoids,
focusing
their
innovative
applications
and
transformative
potential
in
human
health
medicine.
Research
jointly
delves
deeper
into
the
bioactivity
bioavailability
of
revealing
therapeutic
uses
technological
advances
that
have
to
revolutionize
medical
treatments.
We
explore
pioneering
which
carotenoids
are
used
treat
chronic
diseases
such
as
cancer,
cardiovascular
disease,
age-related
macular
degeneration,
offering
novel
protective
mechanisms
benefits.
Our
study
also
shows
cutting-edge
innovations
carotenoid
extraction
bioavailability,
including
development
supramolecular
carriers
advanced
nanotechnology,
dramatically
improve
absorption
efficacy
these
compounds.
These
not
only
ensure
consistent
quality
but
tailor
therapies
each
patient's
needs,
paving
way
for
personalized
By
integrating
latest
scientific
discoveries
techniques,
this
research
provides
prospective
clinical
establishing
new
benchmark
future
studies
field.
findings
underscore
importance
optimizing
extraction,
administration,
bioactivity,
methods
develop
more
effective,
targeted,
treatments,
thus
visionary
insight
modern
practices.
Microorganisms,
Год журнала:
2025,
Номер
13(3), С. 599 - 599
Опубликована: Март 5, 2025
Microbial
engineering
has
made
a
significant
breakthrough
in
pharmaceutical
biotechnology,
greatly
expanding
the
production
of
biologically
active
compounds,
therapeutic
proteins,
and
novel
drug
candidates.
Recent
advancements
genetic
engineering,
synthetic
biology,
adaptive
evolution
have
contributed
to
optimization
microbial
strains
for
applications,
playing
crucial
role
enhancing
their
productivity
stability.
The
CRISPR-Cas
system
is
widely
utilized
as
precise
genome
modification
tool,
enabling
enhancement
metabolite
biosynthesis
activation
biological
pathways.
Additionally,
biology
approaches
allow
targeted
design
microorganisms
with
improved
metabolic
efficiency
potential,
thereby
accelerating
development
new
products.
integration
artificial
intelligence
(AI)
machine
learning
(ML)
plays
vital
further
advancing
by
predicting
network
interactions,
optimizing
bioprocesses,
discovery
process.
However,
challenges
such
efficient
pathways,
ensuring
sustainable
industrial-scale
production,
meeting
international
regulatory
requirements
remain
critical
barriers
field.
Furthermore,
mitigate
potential
risks,
it
essential
develop
stringent
biocontainment
strategies
implement
appropriate
oversight.
This
review
comprehensively
examines
recent
innovations
analyzing
key
technological
advancements,
challenges,
future
perspectives.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Дек. 4, 2024
Over
recent
years,
studies
on
microbiota
research
and
synthetic
biology
have
explored
novel
approaches
microbial
manipulation
for
therapeutic
purposes.
However,
fragmented
information
is
available
this
aspect
with
key
insights
scattered
across
various
disciplines
such
as
molecular
biology,
genetics,
bioengineering,
medicine.
This
review
aims
to
the
transformative
potential
of
in
advancing
microbiome
therapies,
significant
implications
healthcare,
agriculture,
environmental
sustainability.
By
merging
computer
science,
engineering,
allows
precise
design
modification
biological
systems
via
cutting
edge
technologies
like
CRISPR/Cas9
gene
editing,
metabolic
oligonucleotide
synthesis,
thus
paving
way
targeted
treatments
personalized
probiotics
engineered
microorganisms.
The
will
also
highlight
vital
role
gut
disorders
caused
by
its
dysbiosis
suggesting
microbiota-based
therapies
innovations
biosensors
real-time
health
monitoring,
non-invasive
diagnostic
tools,
automated
bio
foundries
better
outcomes.
Moreover,
challenges
including
genetic
stability,
safety,
robust
regulatory
frameworks
be
discussed
understand
importance
ongoing
ensure
safe
effective
interventions.
Genetic
engineering
has
revolutionized
our
ability
to
modify
microorganisms
for
various
applications
in
agriculture,
medicine,
and
industry.
This
review
examines
recent
advances
genetic
techniques
bacteria,
fungi,
oomycetes,
with
a
focus
on
CRISPR-Cas
systems.
In
CRISPR-Cas9
enabled
precise
genome
editing,
enhancing
antibiotic
production
metabolic
engineering.
For
despite
challenges
associated
their
complex
cell
structures,
CRISPR/Cas9
advanced
the
of
enzymes
secondary
metabolites.
significant
plant
pathogens,
modified
Agrobacterium-mediated
transformation,
CRISPR/Cas12a
have
contributed
developing
disease-resistant
crops.
provides
comparative
analysis
efficiencies
across
these
addresses
ethical
regulatory
considerations.
Future
research
directions
include
refining
tools
improve
efficiency
expand
applicability
non-model
organisms.
comprehensive
overview
highlights
transformative
potential
microbiology
its
implications
addressing
global
biotechnology.
Biotechnology for Biofuels and Bioproducts,
Год журнала:
2025,
Номер
18(1)
Опубликована: Март 15, 2025
The
growing
demand
for
sustainable
and
eco-friendly
alternatives
in
bioprocessing,
healthcare,
manufacturing
has
stimulated
significant
interest
Bacillus
subtilis
surface
display
technology.
This
innovative
platform,
leveraging
both
spore
vegetative
cell
forms,
provides
exceptional
versatility
a
wide
spectrum
of
applications,
spanning
from
green
technologies
to
advanced
biomedical
innovations.
robustness
spores
the
metabolic
activity
cells
enable
efficient
enzyme
immobilization,
biocatalysis,
biosensor
development,
facilitating
bioremediation,
pollutant
degradation,
renewable
energy
generation.
Additionally,
B.
systems
have
demonstrated
remarkable
potential
vaccine
development
drug
delivery,
offering
cost-effective,
scalable,
environmentally
alternative
traditional
methods.
These
can
effectively
present
antigens
or
therapeutic
molecules,
enabling
targeted
delivery
robust
immune
responses.
review
explores
recent
advancements,
challenges,
opportunities
harnessing
technology
biomanufacturing,
innovations,
transformative
emphasizing
its
role
addressing
pressing
global
challenges
environmental
sustainability
healthcare.
