Genetically engineered bacteria and microalgae expressing a mutant of cytochrome P450 BM3 for efficient Diuron degradation in wastewater treatment DOI Creative Commons

Christian Helvig,

Thamali Kariyawasam, Bas Vriens

и другие.

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

ABSTRACT The diverse members of the cytochrome P450 superfamily conduct metabolism a wide variety compounds, both endogenous and xenobiotic, thus offering potential for degrading emerging contaminants such as prescription drugs pesticides in wastewater treatment aquaculture. This study demonstrates expression activity mutant enzyme CYP102A1 (BM3 MT35) engineered Bacillus megaterium Chlamydomonas reinhardtii , harnessed degradation herbicide Diuron. BM3 MT35 was transgenically expressed Escherichia coli purified via His6-tag system, with its confirmed by spectral vitro assays. During vivo experiments, transgenic degraded 23% Diuron after 24 h, reaching 65% day 5 Terrific Broth (TB) media. In synthetic municipal wastewater, reached 45% 15%, respectively. No occurred wild-type (WT) all above tested conditions. Additionally, expressing chloroplast also exhibited enhanced (52%) compared to WT strains (6%). Overall, our results demonstrate genetically bacteria microalgae viable strategy potentially new, sustainable alternative approach pollution mitigation. IMPORTANCE With growing number drugs, pesticides, food additives, chemicals produced, is being contaminated an increasing pollutants that cannot be eliminated through classical treatment. New methods should therefore developed remove or deactivate these contaminants. Here, we using mutated enzyme, it possible efficiently metabolize targeted pesticide, this case, These new findings open door ways treat developing low-cost efficient modified microorganisms will able specifically detoxify past water

Язык: Английский

Genetically engineered bacteria and microalgae expressing a mutant of cytochrome P450 BM3 for efficient Diuron degradation in wastewater treatment DOI Creative Commons

Christian Helvig,

Thamali Kariyawasam, Bas Vriens

и другие.

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

ABSTRACT The diverse members of the cytochrome P450 superfamily conduct metabolism a wide variety compounds, both endogenous and xenobiotic, thus offering potential for degrading emerging contaminants such as prescription drugs pesticides in wastewater treatment aquaculture. This study demonstrates expression activity mutant enzyme CYP102A1 (BM3 MT35) engineered Bacillus megaterium Chlamydomonas reinhardtii , harnessed degradation herbicide Diuron. BM3 MT35 was transgenically expressed Escherichia coli purified via His6-tag system, with its confirmed by spectral vitro assays. During vivo experiments, transgenic degraded 23% Diuron after 24 h, reaching 65% day 5 Terrific Broth (TB) media. In synthetic municipal wastewater, reached 45% 15%, respectively. No occurred wild-type (WT) all above tested conditions. Additionally, expressing chloroplast also exhibited enhanced (52%) compared to WT strains (6%). Overall, our results demonstrate genetically bacteria microalgae viable strategy potentially new, sustainable alternative approach pollution mitigation. IMPORTANCE With growing number drugs, pesticides, food additives, chemicals produced, is being contaminated an increasing pollutants that cannot be eliminated through classical treatment. New methods should therefore developed remove or deactivate these contaminants. Here, we using mutated enzyme, it possible efficiently metabolize targeted pesticide, this case, These new findings open door ways treat developing low-cost efficient modified microorganisms will able specifically detoxify past water

Язык: Английский

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