Biomaterials, Год журнала: 2024, Номер 310, С. 122631 - 122631
Опубликована: Май 24, 2024
Язык: Английский
MedComm, Год журнала: 2023, Номер 4(3)
Опубликована: Май 17, 2023
Organoids are three-dimensional (3D) miniaturized versions of organs or tissues that derived from cells with stem potential and can self-organize differentiate into 3D cell masses, recapitulating the morphology functions their in vivo counterparts. Organoid culture is an emerging technology, organoids various tissues, such as brain, lung, heart, liver, kidney, have been generated. Compared traditional bidimensional culture, organoid systems unique advantage conserving parental gene expression mutation characteristics, well long-term maintenance function biological characteristics vitro. All these features open up new opportunities for drug discovery, large-scale screening, precision medicine. Another major application disease modeling, especially hereditary diseases difficult to model vitro modeled by combining genome editing technologies. Herein, we introduce development current advances technology field. We focus on applications basic biology clinical research, also highlight limitations future perspectives. hope this review provide a valuable reference developments organoids.
Язык: Английский
Процитировано
103
Molecular Cancer, Год журнала: 2023, Номер 22(1)
Опубликована: Ноя. 28, 2023
The advent of iPSCs has brought about a significant transformation in stem cell research, opening up promising avenues for advancing cancer treatment. formation is multifaceted process influenced by genetic, epigenetic, and environmental factors. offer distinctive platform investigating the origin cancer, paving way novel approaches to treatment, drug testing, tailored medical interventions. This review article will provide an overview science behind iPSCs, current limitations challenges iPSC-based therapy, ethical social implications, comparative analysis with other types also discuss applications tumorigenesis, future tumorigenesis highlight successful case studies utilizing research. conclusion summarize advancements made research importance continued investment iPSC unlock full potential these cells.
Язык: Английский
Процитировано
66
Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 178, С. 117177 - 117177
Опубликована: Июль 24, 2024
Cytokines regulate immune responses essential for maintaining homeostasis, as deregulated cytokine signaling can lead to detrimental outcomes, including inflammatory disorders. The antioxidants emerge promising therapeutic agents because they mitigate oxidative stress and modulate pathways. Antioxidants potentially ameliorate inflammation-related disorders by counteracting excessive cytokine-mediated responses. A comprehensive understanding of pathways the interplay with is paramount developing natural targeting helping improve clinical outcomes enhance quality life patients. Among these antioxidants, curcumin, vitamin C, D, propolis, allicin, cinnamaldehyde have garnered attention their anti-inflammatory properties potential benefits. This review highlights interrelationship between cytokines-mediated in various diseases approaches involving antioxidants.
Язык: Английский
Процитировано
64
The Innovation, Год журнала: 2024, Номер 5(3), С. 100620 - 100620
Опубликована: Апрель 1, 2024
In the last decade, organoid research has entered a golden era, signifying pivotal shift in biomedical landscape. The year 2023 marked milestone with publication of thousands papers this arena, reflecting exponential growth. However, amid burgeoning expansion, comprehensive and accurate overview field been conspicuously absent. Our review is intended to bridge gap, providing panoramic view rapidly evolving We meticulously analyze from eight distinctive vantage points, harnessing our rich experience academic research, industrial application, clinical practice. present deep exploration advances technology, underpinned by long-standing involvement arena. narrative traverses historical genesis organoids their transformative impact across various sectors, including oncology, toxicology, drug development. delve into synergy between avant-garde technologies such as synthetic biology single-cell omics discuss role tailoring personalized medicine, enhancing high-throughput screening, constructing physiologically pertinent disease models. analysis reflective discourse provide dive existing landscape emerging trends technology. spotlight technological innovations, methodological evolution, broadening spectrum applications, emphasizing revolutionary influence discovery, other fields. Looking ahead, we cautiously anticipate future developments especially its potential implications for patient care, new avenues research. trust that will be an asset researchers, clinicians, patients keen interest medical strategies. offer broad prospective capabilities encompassing wide range current applications. summary, attempt field. reflections, summaries, projections might useful researchers hope contribute shaping trajectory dynamic advancing
Язык: Английский
Процитировано
29
Frontiers in Artificial Intelligence, Год журнала: 2024, Номер 6
Опубликована: Янв. 5, 2024
Human brain organoids, aka cerebral organoids or earlier "mini-brains", are 3D cellular models that recapitulate aspects of the developing human brain. They show tremendous promise for advancing our understanding neurodevelopment and neurological disorders. However, unprecedented ability to model development function
Язык: Английский
Процитировано
22
Biomedicines, Год журнала: 2024, Номер 12(2), С. 397 - 397
Опубликована: Фев. 8, 2024
Non-alcoholic fatty liver disease (NAFLD) is a widespread contributor to chronic globally. A recent consensus on renaming was established, and metabolic dysfunction-associated steatotic disease, MASLD, chosen as the replacement for NAFLD. The disease’s range extends from less severe previously known non-alcoholic (NAFL), more intense steatohepatitis (MASH), (NASH), characterized by inflammation apoptosis. This research project endeavors comprehensively synthesize most studies encompassing wide spectrum of topics such pathophysiology, risk factors, dietary influences, lifestyle management, genetics, epigenetics, therapeutic approaches, prospective trajectory particularly exploring its connection with organoids.
Язык: Английский
Процитировано
22
Molecular Biomedicine, Год журнала: 2024, Номер 5(1)
Опубликована: Фев. 12, 2024
Abstract Cancer is associated with a high degree of heterogeneity, encompassing both inter- and intra-tumor along considerable variability in clinical response to common treatments across patients. Conventional models for tumor research, such as vitro cell cultures vivo animal models, demonstrate significant limitations that fall short satisfying the research requisites. Patient-derived organoids, which recapitulate structures, specific functions, molecular characteristics, genomics alterations expression profiles primary tumors. They have been efficaciously implemented illness portrayal, mechanism exploration, high-throughput drug screening assessment, discovery innovative therapeutic targets potential compounds, customized treatment regimen cancer In contrast conventional organoids offer an intuitive, dependable, efficient model by conserving phenotypic, genetic diversity, mutational attributes originating tumor. Nevertheless, organoid technology also confronts bottlenecks challenges, how comprehensively reflect microenvironment, angiogenesis, reduce costs, establish standardized construction processes while retaining reliability. This review extensively examines use techniques fundamental precision medicine. It emphasizes importance patient-derived biobanks development, screening, safety evaluation, personalized Additionally, it evaluates application experimental better understand mechanisms The intent this explicate significance present new avenues future research.
Язык: Английский
Процитировано
17
Med, Год журнала: 2024, Номер unknown
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
10
Bone Research, Год журнала: 2024, Номер 12(1)
Опубликована: Ноя. 20, 2024
Abstract The concept and development of bone/cartilage organoids are rapidly gaining momentum, providing opportunities for both fundamental translational research in bone biology. Bone/cartilage organoids, essentially miniature tissues grown vitro, enable the study complex cellular interactions, biological processes, disease pathology a representative controlled environment. This review provides comprehensive up-to-date overview field, focusing on strategies organoid construction strategies, progresses research, potential applications. We delve into significance selecting appropriate cells, matrix gels, cytokines/inducers, techniques. Moreover, we explore role advancing our understanding reconstruction, modeling, drug screening, prevention, treatment strategies. While acknowledging these discuss inherent challenges limitations field propose solutions, including use bioprinting induction, AI improved screening exploration assembloids more complex, multicellular models. believe that with continuous refinement standardization, can profoundly impact patient-specific therapeutic interventions lead way regenerative medicine.
Язык: Английский
Процитировано
9
PLoS ONE, Год журнала: 2025, Номер 20(1), С. e0309035 - e0309035
Опубликована: Янв. 23, 2025
Recent advances in bioengineering have made it possible to develop increasingly complex biological systems recapitulate organ functions as closely vitro . Monitoring the assembly and growth of multi-cellular aggregates, micro-tissues or organoids extracting quantitative information is a crucial but challenging task required decipher underlying morphogenetic mechanisms. We present here an imaging platform designed be accommodated inside incubator which provides high-throughput monitoring cell assemblies over days weeks. exemplify capabilities our system by investigating human induced pluripotent stem cells (hiPSCs) enclosed spherical capsules, hiPSCs tubular capsules yeast capsules. Combined with customized pipeline image analysis, solution insight into impact confinement on morphogenesis these self-organized systems.
Язык: Английский
Процитировано
1