Nanoliposomes as nonviral vectors in cancer gene therapy
MedComm,
Год журнала:
2024,
Номер
5(7)
Опубликована: Июнь 25, 2024
Abstract
Nonviral
vectors,
such
as
liposomes,
offer
potential
for
targeted
gene
delivery
in
cancer
therapy.
Liposomes,
composed
of
phospholipid
vesicles,
have
demonstrated
efficacy
nanocarriers
genetic
tools,
addressing
the
limitations
off‐targeting
and
degradation
commonly
associated
with
traditional
therapy
approaches.
Due
to
their
biocompatibility,
stability,
tunable
physicochemical
properties,
they
overcoming
challenges
therapy,
low
transfection
efficiency
poor
stability
biological
fluids.
Despite
these
advancements,
there
remains
a
gap
understanding
optimal
utilization
nanoliposomes
enhanced
treatment.
This
review
delves
into
present
state
carriers
tools
sheds
light
on
safeguard
payloads
facilitate
cell
internalization
alongside
evolution
smart
delivery.
The
linked
biocompatibility
factors
that
restrict
effectiveness
are
also
discussed
along
exploring
strategies
by
analyzing
recent
advancements
offering
future
directions.
Язык: Английский
Lipid-based nanoparticles for nucleic acids delivery
Physical Sciences Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 4, 2025
Abstract
This
chapter
highlights
challenges
and
advancements
in
the
production
of
lipid-based
nanoparticles
(LNPs)
their
application
nucleic
acid-based
therapies.
Recently,
mRNA-based
vaccines
for
COVID-19
immunization
revealed
that
use
acids
is
a
promising
strategy
to
develop
treatments
at
high
therapeutic
efficiency
reduced
side
effects.
In
this
context,
LNPs
emerged
as
favourable
vehicles
delivery
(like
mRNA
DNA),
due
biocompatibility,
bioavailability,
versatility.
The
four
main
components
employed
produce
loaded
with
are:
cationic
or
ionizable
lipids,
helper
cholesterol,
PEGylated
lipids.
Several
conventional
techniques
have
been
proposed
over
years
kind
nanoparticles.
However,
they
show
many
drawbacks
hinder
direct
vesicles
characterized
by
nanometric
size,
encapsulation
active
pharmaceutical
ingredient,
prolonged
stability.
Processes
assisted
supercritical
fluids
(in
particular,
CO
2
)
can
represent
sustainable
interesting
alternative
without
using
post-processing
steps
solvent
removal
size
reduction
are
time-consuming
procedures,
lead
large
loss
acids,
negatively
influence
general
productivity
process.
Язык: Английский
Insight Into the Molecular Parameters of PEI Promoting an Efficient Gene Delivery into Cells
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 3, 2024
Abstract
The
development
of
natural
or
synthetic
polycations
able
to
interact
with
nucleic
acids
and
condense
them
into
nanoparticles
known
as
polyplexes,
faces
several
unresolved
challenges
at
the
cellular
level.
Key
issues
include
intracellular
trafficking
endosomal
escape
release
cytosol,
which
are
considered
major
bottlenecks
for
efficient
protein
expression.
Here,
we
aim
gaining
fundamental
insights
stability
polyplexes
in
biological
media
their
uptake
trafficking,
while
correlating
data
expression
reporter
both
molecular
characteristics
various
poly(ethylenimines)
(PEI)
physicochemical
PEI/peGFP-C3
polyplexes.
For
this,
chosen
four
samples
PEI,
selected
a
model
polycation,
different
weights
(Mw
=
0.8,
20,
25
60
kg/mol)
structures
(linear
branched).
We
found
that
vitro
vivo
cell
internalization
transfection
efficiency
is
dependent
on
variation
polycation
Mw
structure,
well
intrinsic
properties
such
charge
ratio
(R=[N
+
]/[P
−
]).
A
relation
between
percentage
positive
cells
green
fluorescent
(GFP)
amount
internalized
acid
(cyanine
5-peGFP-C3)
allowed
revealing
PEI
promoting
higher
GFP
HEK293T
cells.
In
long
term,
outcome
this
work
will
be
propose
guidelines
help
design
more
effective,
less
cytotoxic
non-viral
gene
carriers
great
potential
new
therapeutic
applications.
Язык: Английский
Research Progress of Phospholipid Vesicles in Biological Field
Biomolecules,
Год журнала:
2024,
Номер
14(12), С. 1628 - 1628
Опубликована: Дек. 19, 2024
Due
to
their
high
biocompatibility,
biodegradability,
and
facile
surface
functionalization,
phospholipid
vesicles
as
carriers
have
garnered
significant
attention
in
the
realm
of
disease
diagnosis
treatment.
On
one
hand,
can
function
probes
for
detection
various
diseases
by
encapsulating
nanoparticles,
thereby
enabling
precise
localization
pathological
changes
monitoring
progression.
other
possess
capability
selectively
target
deliver
therapeutic
agents,
including
drug
molecules,
genes
immune
modulators,
affected
sites,
enhancing
sustained
release
these
agents
improving
efficacy.
Recent
advancements
nanotechnology
led
an
increased
focus
on
application
delivery,
biological
detection,
gene
therapy,
cell
mimics.
This
review
aims
provide
a
concise
overview
structure,
characteristics,
preparation
techniques
varying
sizes.
Furthermore,
we
will
summarize
latest
research
developments
regarding
use
nanomedicines
Additionally,
elucidate
potential
facilitating
internalization,
controlled
release,
targeted
delivery
substrates.
Through
this
review,
aspire
enhance
understanding
evolution
within
field,
outline
prospective
research,
address
forthcoming
challenges
associated
with
Язык: Английский