Investigation of PANoptosis pathway in age-related macular degeneration triggered by Aβ1-40 DOI Creative Commons
Yuxia He, Jing Lu, Yong Du

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 19, 2025

Our study aimed to identify PANoptosis in Aβ1-40-induced AMD, both vivo and vitro, determine if AIM2-PANoptosome mediates this process. We used transcriptomics explore the signaling pathways target genes linked within a mouse model of AMD triggered by Aβ1-40. Optical coherence tomography (OCT), hematoxylin eosin (H&E) staining, electroretinography (ERG) were employed assess retinal damage terms morphology function. Morphological changes ARPE-19 cells observed using optical microscopy scanning electron microscopy. Enzyme-linked immunosorbent assay (ELISA) was detect levels cytokines cell supernatants, orbital serum, human plasma evaluate severity inflammation. CO-immunoprecipitation(CoIP) molecular docking performed impact expression proteins associated with AIM2-PANoptosome. Quantitative polymerase chain reaction (qPCR), Western blot (WB), immunofluorescence, apoptosis detection kits related PANoptosis-like death. results showed that had increased apoptosis, necroptosis, pyroptosis pathways, components. CoIP confirmed AIM2, ZBP1, PYRIN under Aβ1-40 treatment. WB immunofluorescence upregulation PANoptosis-related proteins. Inhibitors reduced Aβ-induced protein expression. ELISA inflammatory cytokines. Apoptosis assays revealed loss morphological changes. In conclusion, displayed death, offering insights into disease pathogenesis.

Язык: Английский

Investigation of PANoptosis pathway in age-related macular degeneration triggered by Aβ1-40 DOI Creative Commons
Yuxia He, Jing Lu, Yong Du

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 19, 2025

Our study aimed to identify PANoptosis in Aβ1-40-induced AMD, both vivo and vitro, determine if AIM2-PANoptosome mediates this process. We used transcriptomics explore the signaling pathways target genes linked within a mouse model of AMD triggered by Aβ1-40. Optical coherence tomography (OCT), hematoxylin eosin (H&E) staining, electroretinography (ERG) were employed assess retinal damage terms morphology function. Morphological changes ARPE-19 cells observed using optical microscopy scanning electron microscopy. Enzyme-linked immunosorbent assay (ELISA) was detect levels cytokines cell supernatants, orbital serum, human plasma evaluate severity inflammation. CO-immunoprecipitation(CoIP) molecular docking performed impact expression proteins associated with AIM2-PANoptosome. Quantitative polymerase chain reaction (qPCR), Western blot (WB), immunofluorescence, apoptosis detection kits related PANoptosis-like death. results showed that had increased apoptosis, necroptosis, pyroptosis pathways, components. CoIP confirmed AIM2, ZBP1, PYRIN under Aβ1-40 treatment. WB immunofluorescence upregulation PANoptosis-related proteins. Inhibitors reduced Aβ-induced protein expression. ELISA inflammatory cytokines. Apoptosis assays revealed loss morphological changes. In conclusion, displayed death, offering insights into disease pathogenesis.

Язык: Английский

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