Reproducibility of PD patient-specific midbrain organoid data for in vitro disease modelling DOI Creative Commons
Elisa Zuccoli,

Haya Al Sawaf,

Mona Tuzza

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Abstract Midbrain organoids are advanced in vitro cellular models for disease modelling. They have been used successfully over the past decade Parkinson’s (PD) research and drug development. The three-dimensional structure multicellular composition allow under more physiological conditions than is possible with conventional 2D models. However, there concerns field regarding organoid batch-to-batch variability thus reproducibility of results. In this manuscript, we generate multiple independent midbrain batches derived from healthy individuals or GBA-N370S mutation-carrying PD patients to evaluate mutation-associated transcriptomic metabolic signature as well selected protein abundance. Our analysis shows that GBA-PD-associated phenotypes reproducible across generation time points. This proves not only suitable modelling, but also represent robust highly

Язык: Английский

Association between triglyceride/high density lipoprotein ratio and incidence risk of Parkinson’s disease: a population-based cohort study DOI Creative Commons
Yoonkyung Chang, Juyoung Park, Tae‐Jin Song

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 16, 2025

Язык: Английский

Процитировано

0
Energy Metabolism and Brain Aging: Strategies to Delay Neuronal Degeneration DOI Creative Commons

Donghui Na,

Z. Zhang,

Meng Meng

и другие.

Cellular and Molecular Neurobiology, Год журнала: 2025, Номер 45(1)

Опубликована: Апрель 21, 2025

Abstract Aging is characterized by a gradual decline in physiological functions, with brain aging being major risk factor for numerous neurodegenerative diseases. Given the brain’s high energy demands, maintaining an adequate ATP supply crucial its proper function. However, advancing age, mitochondria dysfunction and deteriorating metabolism lead to reduced overall production impaired mitochondrial quality control (MQC). As result, promoting healthy has become key focus contemporary research. This review examines relationship between aging, highlighting connection MQC metabolism, proposes strategies delay targeting metabolism.

Язык: Английский

Процитировано

0
Reproducibility of PD patient-specific midbrain organoid data for in vitro disease modelling DOI Creative Commons
Elisa Zuccoli,

Haya Al Sawaf,

Mona Tuzza

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Abstract Midbrain organoids are advanced in vitro cellular models for disease modelling. They have been used successfully over the past decade Parkinson’s (PD) research and drug development. The three-dimensional structure multicellular composition allow under more physiological conditions than is possible with conventional 2D models. However, there concerns field regarding organoid batch-to-batch variability thus reproducibility of results. In this manuscript, we generate multiple independent midbrain batches derived from healthy individuals or GBA-N370S mutation-carrying PD patients to evaluate mutation-associated transcriptomic metabolic signature as well selected protein abundance. Our analysis shows that GBA-PD-associated phenotypes reproducible across generation time points. This proves not only suitable modelling, but also represent robust highly

Язык: Английский

Процитировано

0