N7-methyladenosine-induced SLC7A7 serves as a prognostic biomarker in pan-cancer and promotes CRC progression in colorectal cancer DOI Creative Commons
Fuqi Wang,

Shiqian Zhang,

Zhuang Chen

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Дек. 28, 2024

Abstract Solute transport family 7A member 7 (SLC7A7) mutations contribute to lysinuric protein intolerance (LPI), which is the mechanism of action that has been extensively studied. In colorectal cancer (CRC), SLC7A7 appears play a role, but features and mechanisms are not yet well understood. Survival was analyzed using Kaplan–Meier analysis. Enrichment analysis performed characterize, immune infiltration, methylation, genetic instability, crucial pathways SLC7A7. Afterward, functional experiments were conducted in vitro investigate how affects tumor metastasis. Mechanistically, quantitative real-time PCR (qRT-PCR), western blot (WB), methylated RNA immunoprecipitation (me-RIP) carried out confirm methylation modification related functions. High levels expression predictive worse prognosis for CRC patients. showed significantly enriched during EMT could be Wnt/β-catenin signaling pathway, infiltration pan-cancer macrophages, affected specific cancers. indicated promote migration invasion cells experiments. observed potentially interact with possibly by influencing adenomatous polyposis coli (APC) expression. Furthermore, we identified undergoes N7-methylguanosine (m7G) modification, may regulate mRNA stability, Quaking (QKI) playing role this process recognizing m7G modification. Our results indicate metastasis through SLC7A7/APC/Wnt/β-catenin pathway. Moreover, might involved regulating highlighting novel layer regulation.

Язык: Английский

N7-methyladenosine-induced SLC7A7 serves as a prognostic biomarker in pan-cancer and promotes CRC progression in colorectal cancer DOI Creative Commons
Fuqi Wang,

Shiqian Zhang,

Zhuang Chen

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Дек. 28, 2024

Abstract Solute transport family 7A member 7 (SLC7A7) mutations contribute to lysinuric protein intolerance (LPI), which is the mechanism of action that has been extensively studied. In colorectal cancer (CRC), SLC7A7 appears play a role, but features and mechanisms are not yet well understood. Survival was analyzed using Kaplan–Meier analysis. Enrichment analysis performed characterize, immune infiltration, methylation, genetic instability, crucial pathways SLC7A7. Afterward, functional experiments were conducted in vitro investigate how affects tumor metastasis. Mechanistically, quantitative real-time PCR (qRT-PCR), western blot (WB), methylated RNA immunoprecipitation (me-RIP) carried out confirm methylation modification related functions. High levels expression predictive worse prognosis for CRC patients. showed significantly enriched during EMT could be Wnt/β-catenin signaling pathway, infiltration pan-cancer macrophages, affected specific cancers. indicated promote migration invasion cells experiments. observed potentially interact with possibly by influencing adenomatous polyposis coli (APC) expression. Furthermore, we identified undergoes N7-methylguanosine (m7G) modification, may regulate mRNA stability, Quaking (QKI) playing role this process recognizing m7G modification. Our results indicate metastasis through SLC7A7/APC/Wnt/β-catenin pathway. Moreover, might involved regulating highlighting novel layer regulation.

Язык: Английский

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