Analysis of Metabolic Profiles and Antioxidant Activity of Chinese Cordyceps, Ophiocordyceps sinensis, and Paecilomyces hepiali Based on Untargeted Metabolomics DOI Creative Commons
Min He,

Chuyu Tang,

Tao Wang

и другие.

Biology, Год журнала: 2024, Номер 13(9), С. 683 - 683

Опубликована: Авг. 31, 2024

Chinese cordyceps (GL) is a traditional medicinal fungus, with Ophiocordyceps sinensis (O. sinensis, BL) and Paecilomyces hepiali (P. hepiali, JSB) being fungi isolated from wild cordyceps. These three species share similar chemical composition pharmacological effects. Existing studies have primarily compared the metabolites of O. overlooking assessment antioxidant capacity in cordyceps, P. sinensis. In this study, LC-MS/MS was employed to analyze GL, JSB, BL. Utilizing principal component analysis (PCA), supervised orthogonal partial least squares discriminant (OPLS-DA), hierarchical cluster (HCA), it observed that majority differential (DMs) accumulated organic acids derivatives, lipids lipid-like molecules, organoheterocyclic compounds. Antioxidant activity indicated GL exhibited higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability (DPPH•, rate 81.87 ± 0.97%), hydroxyl free (•OH, 98.10 0.60%), superoxide anion (O2•−, 69.74 4.36%), while JSB demonstrated FRAP total 8.26 μmol Trolox/g (p < 0.05). Correlation revealed positive correlation between DMs (fatty acyls amino acids) DPPH•, FRAP, •OH, O2•− Additionally, glycerophospholipid were found be positively correlated Through KEGG pathway analysis, determined accumulation pathways such as cutin, suberine wax biosynthesis has impact on influencing samples. results shed light metabolic characteristics its substitutes offer valuable insights into how different strength activity, aiding advancement application substitutes.

Язык: Английский

Metabolomics and Transcriptomics Reveal the Effects of Different Fermentation Times on Antioxidant Activities of Ophiocordyceps sinensis DOI Creative Commons
Min He,

Tao Wang,

Chuyu Tang

и другие.

Journal of Fungi, Год журнала: 2025, Номер 11(1), С. 51 - 51

Опубликована: Янв. 9, 2025

Ophiocordyceps sinensis is a fungus that cultured through fermentation from wild Chinese cordyceps. While studies have examined its metabolites, the evaluation of antioxidant capacity remains to be conducted. The results O. indicate ferric ion-reducing power (FRAP), (2.74 ± 0.12 μmol Trolox/g), 2,2-diphenyl-1-picrylhydrazyl (DPPH•) free radical scavenging rate (60.21 0.51%), and hydroxyl (91.83 0.68%) reached maximum on day 30. Using LC-MS/MS measure metabolites D24, D30, D36, we found majority differential accumulated (DAMs) primarily accumulate in lipids, organoheterocyclic compounds, organic acids their derivatives. Notably, DAMs exhibiting high peaks include acetylcarnitine, glutathione, linoleic acid, L-propionylcarnitine, among others. transcriptome analysis differentially expressed genes (DEGs) expression D30 included lnaA, af470, ZEB1; D24 comprised SPBC29A3.09c YBT1; D36 dtxS1, PA1538, katG. combined revealed significant extremely positive negative correlations between all DEGs. primary enriched pathways (p < 0.05) glutathione metabolism, tryptophan carbon biosynthesis secondary phenylalanine metabolism. metabolic pathway map DEGs influencing activity were significantly up-regulated but down-regulated D36. correlation suggests an increase content promotes levels enzyme non-enzyme substances, ultimately enhancing sinensis. These findings serve as reference how affect This may contribute enhanced development application

Язык: Английский

Процитировано

1
Metabolomics analysis reveals the changes of metabolites in Cordyceps militaris during subculture DOI
Xin Wang, Yefei Liu, Shuang Yu

и другие.

Food Chemistry, Год журнала: 2025, Номер 479, С. 143863 - 143863

Опубликована: Март 14, 2025

Язык: Английский

Процитировано

0
Cordyceps Sinensis Reduces Inflammation and Protects BEAS-2B Cells From LPS-Induced THP-1 Cell Injury DOI Creative Commons
Xiaqing Wu, Xin Li, Ying Chai

и другие.

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 4143 - 4156

Опубликована: Март 1, 2025

Cordyceps sinensis, an entomogenous fungus with unique biological properties, has demonstrated significant anti-inflammatory potential. However, its effects on inflammation regulation need to be further investigated in detail. In this study, we aimed analyze the sinensis extract (CSE) obtained via ethanol extraction and assess regulation. The secretion of pro-inflammatory cytokines (IL-6, TNF-α, IL-8, IL-1β) level MMP9, Nrf2/HO-1 ROS were evaluated. A transwell system THP-1 BEAS-2B cells was used explore inflammatory damage. Gene ontology (GO) Kyoto encyclopedia genes genomes (KEGG) enrichment analyses conducted differentially expressed genes. CSE exhibited no cytotoxicity at concentrations ≤ 1.6 mg/mL. Treatment LPS-induced significantly inhibited cytokines. reduced inflammation-related protein while upregulating signaling pathway. Fluorescence assays using DCF JC-1 confirmed that help mitigate oxidative stress-induced inflammation. treatment protected from Moreover, immune process a shared GO term between LPS-only combined LPS treatment. KEGG analysis showed is capable regulating associated responses. These findings highlight potential as immune-regulating agent functional foods health products.

Язык: Английский

Процитировано

0
Analysis of Metabolic Profiles and Antioxidant Activity of Chinese Cordyceps, Ophiocordyceps sinensis, and Paecilomyces hepiali Based on Untargeted Metabolomics DOI Creative Commons
Min He,

Chuyu Tang,

Tao Wang

и другие.

Biology, Год журнала: 2024, Номер 13(9), С. 683 - 683

Опубликована: Авг. 31, 2024

Chinese cordyceps (GL) is a traditional medicinal fungus, with Ophiocordyceps sinensis (O. sinensis, BL) and Paecilomyces hepiali (P. hepiali, JSB) being fungi isolated from wild cordyceps. These three species share similar chemical composition pharmacological effects. Existing studies have primarily compared the metabolites of O. overlooking assessment antioxidant capacity in cordyceps, P. sinensis. In this study, LC-MS/MS was employed to analyze GL, JSB, BL. Utilizing principal component analysis (PCA), supervised orthogonal partial least squares discriminant (OPLS-DA), hierarchical cluster (HCA), it observed that majority differential (DMs) accumulated organic acids derivatives, lipids lipid-like molecules, organoheterocyclic compounds. Antioxidant activity indicated GL exhibited higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability (DPPH•, rate 81.87 ± 0.97%), hydroxyl free (•OH, 98.10 0.60%), superoxide anion (O2•−, 69.74 4.36%), while JSB demonstrated FRAP total 8.26 μmol Trolox/g (p < 0.05). Correlation revealed positive correlation between DMs (fatty acyls amino acids) DPPH•, FRAP, •OH, O2•− Additionally, glycerophospholipid were found be positively correlated Through KEGG pathway analysis, determined accumulation pathways such as cutin, suberine wax biosynthesis has impact on influencing samples. results shed light metabolic characteristics its substitutes offer valuable insights into how different strength activity, aiding advancement application substitutes.

Язык: Английский

Процитировано

3