Multi-strategy ugt mining, modification and glycosyl donor synthesis facilitate the production of triterpenoid saponins DOI Creative Commons
Lin Hao, Yu Liu, Guangbin Dong

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Май 30, 2025

Triterpenoid saponins are a class of plant secondary metabolites with significant biological activities and widely used in the pharmaceutical nutritional supplement industries. However, production triterpenoid is limited by their complicated biosynthetic pathways availability glycosyl donors. UDP-glycosyltransferases (UGTs) play key role glycosylation saponins, significantly enhancing structural diversity, solubility, pharmacological activity, bioavailability. Therefore, identification modification efficient, specific, stable UGTs have attracted attention. This review focused on advances particular emphasis application multi-omics approaches UGT mining. The combination genomics, transcriptomics, metabolomics has provided powerful tools for screening, improving efficiency accuracy identification. Additionally, methods based gene clusters, phylogenetic analysis, product glycosyltransferase (PSPG) motif also offer new perspectives Besides, synthetic biology platforms innovative high-throughput screening functional validation UGTs, laying theoretical foundation UGTs. We discussed latest research progress including directed evolution rational design. These strategies, through amino acid mutations optimization, expected to enhance catalytic thermal stability, broaden substrate specificity. Moreover, diversity donors directly influence reactions products. Thus, we donor synthesis, vitro vivo strategies. By optimizing metabolic introducing enzyme genes, engineered microorganisms can efficiently synthesize various donors, providing abundant substrates reactions. studies opportunities challenges synthesis promoting industrial potential.

Язык: Английский

Biochemical Characterization and Mechanism of Thermostability of the Thermophilic Hyaluronate Lyase TcHly8D DOI
Yuzhu Zhang, Hao Wu, Zheng Fu

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Hyaluronate lyases are widely used in medicine and biochemical engineering also applied as a tool enzyme to prepare oligosaccharides with various biological activities. To date, only few hyaluronate on sale poor thermostability. In this study, PL8 lyase, TcHly8D, was found from Thermasporomyces composti expressed Escherichia coli maximum yield of 1.77 × 109 U/L (3.14 g/L) 5-L bioreactor. The recombinant TcHly8D exhibited high lyase activity 5.64 105 U/mg an excellent thermostability half-lives 184.9 h at 60 °C. Fifty micrograms could catalyze 5 g hyaluronic acid oligosaccharide 84.8% 4 h. salt bridges, hydrogen bonds, proline residues, but not disulfide played important roles the TcHly8D. These findings provide insights into multifunctional application potential agriculture, medicine, food industry.

Язык: Английский

Процитировано

1
Exploring Enzyme Thermostability Enhancement through Molecular Modification: What Remains to be Known? DOI

Ammar Khazaal Kadhim Almansoori,

Kang Yu,

Shallinie Thangadurai

и другие.

Journal of Molecular Structure, Год журнала: 2025, Номер unknown, С. 141852 - 141852

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Improving the catalytic activity and thermostability of Mariniflexile fucanivorans fucoidanase by rational design DOI
Huilin Chen, Sijia Huang,

Xiaolan Wang

и другие.

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106873 - 106873

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Multi-strategy ugt mining, modification and glycosyl donor synthesis facilitate the production of triterpenoid saponins DOI Creative Commons
Lin Hao, Yu Liu, Guangbin Dong

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Май 30, 2025

Triterpenoid saponins are a class of plant secondary metabolites with significant biological activities and widely used in the pharmaceutical nutritional supplement industries. However, production triterpenoid is limited by their complicated biosynthetic pathways availability glycosyl donors. UDP-glycosyltransferases (UGTs) play key role glycosylation saponins, significantly enhancing structural diversity, solubility, pharmacological activity, bioavailability. Therefore, identification modification efficient, specific, stable UGTs have attracted attention. This review focused on advances particular emphasis application multi-omics approaches UGT mining. The combination genomics, transcriptomics, metabolomics has provided powerful tools for screening, improving efficiency accuracy identification. Additionally, methods based gene clusters, phylogenetic analysis, product glycosyltransferase (PSPG) motif also offer new perspectives Besides, synthetic biology platforms innovative high-throughput screening functional validation UGTs, laying theoretical foundation UGTs. We discussed latest research progress including directed evolution rational design. These strategies, through amino acid mutations optimization, expected to enhance catalytic thermal stability, broaden substrate specificity. Moreover, diversity donors directly influence reactions products. Thus, we donor synthesis, vitro vivo strategies. By optimizing metabolic introducing enzyme genes, engineered microorganisms can efficiently synthesize various donors, providing abundant substrates reactions. studies opportunities challenges synthesis promoting industrial potential.

Язык: Английский

Процитировано

0