Dopamine and Citicoline-Co-Loaded Solid Lipid Nanoparticles as Multifunctional Nanomedicines for Parkinson’s Disease Treatment by Intranasal Administration
Pharmaceutics,
Год журнала:
2024,
Номер
16(8), С. 1048 - 1048
Опубликована: Авг. 7, 2024
This
work
aimed
to
evaluate
the
potential
of
nanosystems
constituted
by
dopamine
(DA)
and
antioxidant
Citicoline
(CIT)
co-loaded
in
solid
lipid
nanoparticles
(SLNs)
for
intranasal
administration
treatment
Parkinson
disease
(PD).
Such
nanosystems,
denoted
as
DA-CIT-SLNs,
were
designed
according
concept
multifunctional
nanomedicine
where
multiple
biological
roles
are
combined
into
a
single
nanocarrier
prepared
melt
emulsification
method
employing
self-emulsifying
Gelucire®
50/13
matrix.
The
resulting
DA-CIT-SLNs
characterized
regarding
particle
size,
surface
charge,
encapsulation
efficiency,
morphology,
physical
stability.
Differential
scanning
calorimetry,
FT-IR,
X
ray
diffraction
studies
carried
out
gain
information
on
solid-state
features,
vitro
release
tests
simulated
nasal
fluid
(SNF)
performed.
Monitoring
size
at
two
temperatures
(4
°C
37
°C),
enlargement
observed
over
time
was
lower
than
that
4
°C,
even
though
higher
temperature,
color
changes
occurred,
indicative
possible
neurotransmitter
decomposition.
Solid-state
indicated
reduction
crystallinity
when
DA
CIT
co-encapsulated
DA-CIT-SLNs.
Interestingly,
SNF
sustained
DA.
Furthermore,
DA-CIT
SLNs
displayed
high
cytocompatibility
with
both
human
RPMI
2650
neuronal
SH-SY5Y
cells.
OxyBlot
assay
demonstrated
considerable
assess
protective
effect
agents
against
oxidative
cellular
damage.
Thus,
such
shown
which
constitute
promising
formulation
PD
application.
Язык: Английский
Tailored Intranasal Albumin Caged Selegiline-α Synuclein siRNA Liposome with Improved Efficiency in Parkinson’s Model
Pharmaceutics,
Год журнала:
2025,
Номер
17(2), С. 243 - 243
Опубликована: Фев. 12, 2025
Background/Objectives:
Parkinson's
disease
(PD)
is
a
progressive
neuro-degenerative
disorder
characterized
by
α-synuclein
aggregation,
which
promotes
neuronal
death
and
accelerates
neurodegeneration.
Small
interfering
RNA
(siRNA)
can
reduce
levels,
but
its
therapeutic
potential
limited
poor
stability
delivery
challenges.
Similarly,
Selegiline
(Sel),
monoamine
oxidase-B
(MAO-B)
inhibitor,
has
low
bioavailability,
restricting
effectiveness.
This
study
aims
to
develop
an
intranasal
(IN)
albumin-coated
liposomal
system
(C-LipSel-siSNCA2)
for
the
co-delivery
of
Sel
α-synuclein-targeting
siRNA
(siSNCA2)
enhance
brain
targeting
efficacy.
Methods:
Liposomes
were
prepared
using
ethanol
injection
method
optimized
via
D-optimal
design
size,
charge,
encapsulation
efficiency
(EE%).
The
formulation
was
coated
with
human
serum
albumin
(HSA)
stability,
cellular
uptake,
gene
silencing.
In
vivo
pharmacokinetics
pharmacodynamics
assessed
in
rotenone-induced
PD
rat
model
evaluate
motor
function,
biochemical
markers,
brain-targeting
efficiency.
Results:
Optimized
liposomes
had
particle
size
113.5
±
6.8
nm,
zeta
6.2
0.8
mV,
high
EE%
(Sel:
92.35%;
siRNA:
78.66%).
Albumin
coating
increased
136.5
10.3
nm
shifted
-13.5
1.4
enhancing
targeting.
IN
administration
achieved
3-fold
increase
area
under
concentration-time
curve
(AUC)
versus
intravenous
delivery.
rats,
C-LipSel-siSNCA2
improved
non-motor
functions,
restored
dopamine
enhanced
catalase
activity,
reduced
MAO-B
mitigating
degradation
aggregation.
Conclusions:
non-invasive,
dual-action
nanoplatform
offers
targeted
therapy
PD,
combining
silencing
inhibition,
clinical
translation
neurodegenerative
diseases.
Язык: Английский
Lipid-Based Nanoparticles for Neurological Drug Discovery
Nanotechnology in the life sciences,
Год журнала:
2025,
Номер
unknown, С. 283 - 301
Опубликована: Янв. 1, 2025
Язык: Английский
Mitochondrial-based therapies for neurodegenerative diseases: a review of the current literature
Naunyn-Schmiedeberg s Archives of Pharmacology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 31, 2025
Abstract
Neurodegenerative
disorders
present
significant
challenges
to
modern
medicine
because
of
their
complex
etiology,
pathogenesis,
and
progressive
nature,
which
complicate
practical
treatment
approaches.
Mitochondrial
dysfunction
is
an
important
contributor
the
pathophysiology
various
neurodegenerative
illnesses,
including
Alzheimer’s
disease
(AD),
Parkinson’s
(PD),
amyotrophic
lateral
sclerosis
(ALS).
This
review
paper
examines
current
literature
highlighting
multifaceted
functions
mitochondria,
energy
production,
calcium
signaling,
apoptosis
regulation,
mitochondrial
biogenesis,
dynamics,
axonal
transport,
endoplasmic
reticulum–mitochondrial
interactions,
mitophagy,
proteostasis,
crucial
involvement
in
neuronal
health.
The
emphasizes
increasing
recognition
as
a
critical
factor
progression
disorders,
marking
shift
from
traditional
symptom
management
innovative
mitochondrial-based
therapies.
By
discussing
mechanisms,
quality
control
(MQC)
processes
impact
oxidative
stress,
this
highlights
need
for
novel
therapeutic
strategies
restore
function,
protect
connections
integrity,
slow
progression.
comprehensive
aims
provide
insights
into
potential
interventions
that
could
transform
landscape
diseases,
addressing
symptoms
underlying
pathophysiological
changes.
Язык: Английский
Nanotechnological approaches for efficient N2B delivery: from small-molecule drugs to biopharmaceuticals
Beilstein Journal of Nanotechnology,
Год журнала:
2024,
Номер
15, С. 1400 - 1414
Опубликована: Ноя. 12, 2024
Central
nervous
system
diseases
negatively
affect
patients
and
society.
Providing
successful
noninvasive
treatments
for
these
is
challenging
because
of
the
presence
blood–brain
barrier.
While
protecting
brain’s
homeostasis,
barrier
limits
passage
almost
all
large-molecule
drugs
most
small-molecule
drugs.
A
method,
nose-to-brain
delivery
(N2B
delivery)
has
been
proposed
to
overcome
this
challenge.
By
exploiting
direct
anatomical
interaction
between
nose
brain,
can
reach
target,
brain.
Moreover,
be
encapsulated
into
various
drug
systems
enhance
physicochemical
characteristics
targeting
success.
Many
preclinical
data
show
that
strategy
effectively
deliver
biopharmaceuticals
Therefore,
review
focuses
on
N2B
while
giving
examples
different
suitable
applications.
In
addition,
we
emphasize
importance
effective
monoclonal
antibodies
RNA
stress
recent
literature
tackling
nanotechnological
approaches
small
or
large
molecules
from
current
literature,
highlight
studies
their
results
prove
strategies’
success
limitations.
Язык: Английский
Understanding the nose–brain axis and its role in related diseases: A conceptual review
Neurobiology of Disease,
Год журнала:
2024,
Номер
202, С. 106690 - 106690
Опубликована: Окт. 9, 2024
The
nose-brain
axis
(NBA),
a
critical
component
of
the
body-brain
axis,
not
only
serves
as
drug
transport
route
for
treatment
brain
diseases
but
also
mediates
changes
such
neuroimmune
disorders,
which
may
be
an
important
mechanism
in
occurrence
and
development
some
nasal
or
diseases.
Despite
its
importance,
there
are
substantial
gaps
that
remain
our
understanding
characteristics
NBA-mediated
cellular
molecular
mechanisms
underlying
bidirectional
NBA
crosstalk.
These
have
limited
translational
application
NBA-related
research
findings
to
extent.
Therefore,
this
review
aims
address
conceptual
framework
highlight
values
representative
by
combining
existing
literature
with
new
results
from
group.
We
hope
paper
will
provide
basis
further
in-depth
field,
facilitate
clinical
translation
NBA.
Язык: Английский