Convergent antibody responses to SARS-CoV-2 in convalescent individuals

Nature, Год журнала: 2020, Номер 584(7821), С. 437 - 442

Опубликована: Июнь 18, 2020

During the coronavirus disease-2019 (COVID-19) pandemic, severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has led to infection of millions people and claimed hundreds thousands lives. The entry virus into cells depends on receptor-binding domain (RBD) spike (S) protein SARS-CoV-2. Although there is currently no vaccine, it likely that antibodies will be essential for protection. However, little known about human antibody response SARS-CoV-21–5. Here we report 149 COVID-19-convalescent individuals. Plasma samples collected an average 39 days after onset symptoms had variable half-maximal pseudovirus neutralizing titres; titres were less than 50 in 33% samples, below 1,000 79% only 1% above 5,000. Antibody sequencing revealed expansion clones RBD-specific memory B expressed closely related different Despite low plasma titres, three distinct epitopes RBD neutralized with inhibitory concentrations (IC50 values) as 2 ng ml−1. In conclusion, most convalescent obtained from individuals who recover COVID-19 do not contain high levels activity. Nevertheless, rare but recurring potent antiviral activity found all tested, suggesting a vaccine designed elicit such could broadly effective. rare, against SARS-CoV-2 showed tested individuals,

Язык: Английский

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Dysregulated Type I Interferon and Inflammatory Monocyte-Macrophage Responses Cause Lethal Pneumonia in SARS-CoV-Infected Mice

Cell Host & Microbe, Год журнала: 2016, Номер 19(2), С. 181 - 193

Опубликована: Фев. 1, 2016

Язык: Английский

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Coronavirus disease 2019: What we know?

Journal of Medical Virology, Год журнала: 2020, Номер 92(7), С. 719 - 725

Опубликована: Март 14, 2020

In late December 2019, a cluster of unexplained pneumonia cases has been reported in Wuhan, China. A few days later, the causative agent this mysterious was identified as novel coronavirus. This virus temporarily named severe acute respiratory syndrome coronavirus 2 and relevant infected disease 2019 (COVID-19) by World Health Organization, respectively. The COVID-19 epidemic is spreading China all over world now. purpose review primarily to pathogen, clinical features, diagnosis, treatment COVID-19, but also comment briefly on epidemiology pathology based current evidence.

Язык: Английский

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Coagulation disorders in coronavirus infected patients: COVID-19, SARS-CoV-1, MERS-CoV and lessons from the past

Journal of Clinical Virology, Год журнала: 2020, Номер 127, С. 104362 - 104362

Опубликована: Апрель 10, 2020

Язык: Английский

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COVID-19: what has been learned and to be learned about the novel coronavirus disease

International Journal of Biological Sciences, Год журнала: 2020, Номер 16(10), С. 1753 - 1766

Опубликована: Янв. 1, 2020

The outbreak of Coronavirus disease 2019 , caused by severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2), has thus far killed over 3,000 people and infected 80,000 in China elsewhere the world, resulting catastrophe for humans.Similar to its homologous virus, SARS-CoV, which SARS thousands 2003, SARS-CoV-2 might also be transmitted from bats causes similar symptoms through a mechanism.However, COVID-19 lower severity mortality than but is much more transmissive affects elderly individuals youth men women.In response rapidly increasing number publications on emerging disease, this article attempts provide timely comprehensive review swiftly developing research subject.We will cover basics about epidemiology, etiology, virology, diagnosis, treatment, prognosis, prevention disease.Although many questions still require answers, we hope that helps understanding eradication threatening disease.

Язык: Английский

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Structures of Human Antibodies Bound to SARS-CoV-2 Spike Reveal Common Epitopes and Recurrent Features of Antibodies

Cell, Год журнала: 2020, Номер 182(4), С. 828 - 842.e16

Опубликована: Июнь 24, 2020

Neutralizing antibody responses to coronaviruses mainly target the receptor-binding domain (RBD) of trimeric spike. Here, we characterized polyclonal immunoglobulin Gs (IgGs) and Fabs from COVID-19 convalescent individuals for recognition coronavirus spikes. Plasma IgGs differed in their focus on RBD epitopes, alpha- beta-coronaviruses, contributions avidity increased binding/neutralization over Fabs. Using electron microscopy, examined specificities plasma Fabs, revealing both S1A epitopes SARS-CoV-2 Moreover, a 3.4 Å cryo-electron microscopy (cryo-EM) structure neutralizing monoclonal Fab-spike complex revealed an epitope that blocks ACE2 receptor binding. Modeling based these structures suggested different potentials inter-spike crosslinking by viruses, would not be affected identified spike mutations. Overall, our studies structurally define recurrent anti-SARS-CoV-2 class derived VH3-53/VH3-66 similarity SARS-CoV VH3-30 antibody, providing criteria evaluating vaccine-elicited antibodies.

Язык: Английский

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Molecular Evolution of Human Coronavirus Genomes

Trends in Microbiology, Год журнала: 2016, Номер 25(1), С. 35 - 48

Опубликована: Окт. 19, 2016

Язык: Английский

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Can the Coronavirus Disease 2019 (COVID-19) Affect the Eyes? A Review of Coronaviruses and Ocular Implications in Humans and Animals

Ocular Immunology and Inflammation, Год журнала: 2020, Номер 28(3), С. 391 - 395

Опубликована: Март 16, 2020

In December 2019, a novel coronavirus (CoV) epidemic, caused by the severe acute respiratory syndrome – 2 (SARS-CoV-2) emerged from China. This virus causes disease 2019 (COVID-19). Since then, there have been anecdotal reports of ocular infection. The implications human CoV infections not widely studied. However, CoVs known to cause various in animals. Clinical entities such as conjunctivitis, anterior uveitis, retinitis, and optic neuritis documented feline murine models. this article, current evidence suggesting possible infection tissue is reviewed. review article will also highlight animal their associated infections. We hope that serve start for further research into

Язык: Английский

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Continuous and Discontinuous RNA Synthesis in Coronaviruses

Annual Review of Virology, Год журнала: 2015, Номер 2(1), С. 265 - 288

Опубликована: Ноя. 7, 2015

Replication of the coronavirus genome requires continuous RNA synthesis, whereas transcription is a discontinuous process unique among viruses. Transcription includes template switch during synthesis subgenomic negative-strand RNAs to add copy leader sequence. Coronavirus regulated by multiple factors, including extent base-pairing between transcription-regulating sequences positive and negative polarity, viral cell protein–RNA binding, high-order RNA-RNA interactions. performed replication-transcription complex that proteins recognize cis-acting elements mainly located in highly structured 5′ 3′ untranslated regions. In addition many nonstructural proteins, presence nuclear nucleocapsid protein increases virus amplification efficacy. connected with formation double-membrane vesicles convoluted membranes. Coronaviruses encode proofreading machinery, world, ensure maintenance their large size.

Язык: Английский

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From SARS and MERS to COVID-19: a brief summary and comparison of severe acute respiratory infections caused by three highly pathogenic human coronaviruses

Respiratory Research, Год журнала: 2020, Номер 21(1)

Опубликована: Авг. 27, 2020

Abstract Within two decades, there have emerged three highly pathogenic and deadly human coronaviruses, namely SARS-CoV, MERS-CoV SARS-CoV-2. The economic burden health threats caused by these coronaviruses are extremely dreadful getting more serious as the increasing number of global infections attributed deaths SARS-CoV-2 MERS-CoV. Unfortunately, specific medical countermeasures for hCoVs remain absent. Moreover, fast spread misinformation about ongoing pandemic uniquely places virus alongside an annoying infodemic causes unnecessary worldwide panic. shares many similarities with SARS-CoV MERS-CoV, certainly, obvious differences exist well. Lessons learnt from timely updated information summarized knowledge invaluable effectively efficiently contain outbreak By gaining a deeper understanding illnesses them, we can bridge gaps, provide cultural weapons fighting controling SARS-CoV-2, prepare effective robust defense lines against that may emerge or reemerge in future. To this end, state-of-the-art comparing biological features lethal clinical characteristics them systematically review.

Язык: Английский

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