Fingerprint profiling for quality evaluation and the related biological activity analysis of polysaccharides from Liuweizhiji Gegen-Sangshen beverage
Frontiers in Nutrition,
Год журнала:
2024,
Номер
11
Опубликована: Июль 8, 2024
Introduction
Liuweizhiji
Gegen-Sangshen
beverage
(LGS)
is
popular
in
China,
which
has
been
used
for
alleviating
alcohol-mediated
discomfort
and
preventing
alcoholic
liver
disease
(ALD).
This
consisted
of
six
herbal
components
that
are
known
as
functional
foods
fruits.
LGS
rich
polysaccharides,
however,
the
activity
quality
evaluation
LGS-derived
polysaccharides
remain
unexplored.
The
purpose
this
study
thus
to
establish
a
comprehensive
control
methodology
assessment
(LGSP)
further
explore
anti-oxidant,
anti-inflammatory
well
prebiotic
effect
LGSP.
Methods
LGSP
was
extracted,
followed
by
analysis
molecular
weight
distribution,
monosaccharide
content
structural
characterization
via
integrating
application
high-performance
size
exclusion
chromatography
(HPSEC),
1-phenyl-3-methyl-5-pyrazolone-HPLC
(PMP-HPLC),
fourier
transform
infrared
spectroscopy
(FT-IR)
nuclear
magnetic
resonance
(NMR)
techniques.
anti-oxidation
determined
DPPH,
ABTS,
hydroxyl
radical
scavenging
capacity
total
antioxidant
capacity.
anti-inflammation
were
assessed
on
RAW
264.7
cells.
growth
Lactobacillus,
Bifidobacterium
bifidum
adolescentis
evaluated.
Results
results
demonstrated
had
two
distribution
peaks,
with
average
weights
(6.569
±
0.12)
×
10
4
Da
(4.641
0.30)
Da.
composed
8
monosaccharides,
galacturonic
acid,
glucose
rhamnose
galactose
representing
highest
molar
ratios.
Homogalacturonic
acid
(HG)
type
rhamnosegalacturonic
glycans
I
(RG-I)
α-1,4-glucan
present
concentration
17.94
0.28
mg/mL.
Furthermore,
fingerprint
combined
composition
quantification
batches
there
high
similarity
among
batches.
Notably,
exhibited
anti-oxidant
inhibited
expressions
pro-inflammatory
factors
(TNF-α
IL-6)
LPS-stimulated
In
addition,
remarkably
promoted
proliferation
probiotics
adolescentis,
showing
good
activity.
Discussion
would
be
help
gain
understanding
structure–activity
relationship
LGSP,
provide
reference
bioactive
facilitate
development
unique
health
products
future.
Язык: Английский
Modern Analytical Techniques for Quality Control and Chemical Identification of Phytochemicals
Опубликована: Март 13, 2025
Язык: Английский
Structural Elucidation and NMR Spectral Assignments of New Diphenyl Ether Derivatives From Liuweizhiji Gegen‐Sangshen Oral Liquid
Magnetic Resonance in Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 18, 2024
ABSTRACT
Chemical
investigation
of
the
Liuweizhiji
Gegen‐Sangshen
oral
liquid
afforded
one
new
diphenyl
ether
derivative
(
1
),
together
with
known
compound
2
).
Their
structures
were
established
by
1D
and
2D
NMR,
HR‐ESI‐MS
spectroscopic
analysis
absolute
configuration
was
confirmed
ECD
calculation.
Compounds
evaluated
for
cytotoxic
activities,
compounds
showed
weak
activities
towards
HepG2
human
liver
cancer
cells,
IC
50
values
97.3
79.6
μM,
respectively.
Язык: Английский
Liuweizhiji Gegen-Sangshen beverage protects against alcoholic liver disease in mice through the gut microbiota mediated SCFAs/GPR43/GLP-1 pathway
Frontiers in Nutrition,
Год журнала:
2024,
Номер
11
Опубликована: Дек. 13, 2024
Introduction
Alcoholic
liver
disease
(ALD)
is
a
pathological
state
of
the
caused
by
longterm
alcohol
consumption.
Recent
studies
have
shown
that
modulation
gut
microbiota
and
its
metabolic
products,
specifically
short-chain
fatty
acids
(SCFAs),
exert
critical
role
in
evolution
progression
ALD.
The
Liuweizhiji
Gegen-Sangshen
beverage
(LGS),
as
functional
China,
derived
from
traditional
Chinese
herbal
formula
has
been
clinically
applied
for
ALD
treatment,
demonstrating
significant
efficacy.
However,
underlying
mechanisms
LGS
alleviating
involving
regulation
remain
unknown.
Methods
In
this
study,
an
murine
model
based
on
National
Institute
Alcohol
Abuse
Alcoholism
(NIAAA)
method
was
established.
Results
results
showed
oral
treatment
dose-dependently
alleviated
alcoholinduced
injury
inflammation
mice
through
decreasing
levels
ALT,
AST
proinflammatory
cytokines
(TNF-α,
IL-6,
IL-1β).
significantly
improved
steatosis,
enhanced
activities
metabolizing
enzymes
(ALDH
ADH),
reduced
CYP2E1
activity.
Notably,
regarding
most
detected
indices,
effect
(particularly
at
medium
high
dose)
comparable
to
positive
drug
MTDX.
Moreover,
had
favorable
maintaining
intestinal
barrier
function
reducing
epithelial
increasing
expression
occludin.
16S
rRNA
sequencing
remarkably
modulated
structure
via
recovering
alcohol-induced
microbial
changes
mediating
enrichment
several
bacterial
genera
(
Alloprevotella
,
Monoglobus
Erysipelatoclostridium
Parasutterella,
Harryflintia
unclassified_c_Clostridia
).
Further
study
revealed
increased
production
SCFAs
hexanoic
acid
cecum,
promoted
alcohol-mediated
reduction
GRP43
ileum,
serum
GLP-1
level.
Discussion
Overall,
exerts
remarkable
protective
mediated
specific
GPR43/GLP-1
pathway.
Язык: Английский
Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota
Chinese Medicine,
Год журнала:
2024,
Номер
19(1)
Опубликована: Дек. 23, 2024
Abstract
Background
Liuweizhiji
Gegen-Sangshen
oral
liquid
(LGS),
as
a
Chinese
medicinal
preparation,
is
developed
from
Traditional
formula
consisting
of
six
herbs,
including
Puerariae
lobatae
radix,
Hoveniae
semen,
Imperatae
rhizoma,
Crataegi
fructus,
Mori
fructus
and
Canarli
has
been
extensively
utilized
in
the
prevention
treatment
alcoholic
liver
disease
(ALD)
clinically.
Previous
study
demonstrated
that
LGS
dose-dependently
mitigated
ALD
rat
models.
However,
whether
how
main
characteristic
constituents
(the
flavonoid
polysaccharide
fractions,
LGSF
LGSP)
contribute
to
anti-ALD
effect
remains
unclear.
This
aimed
assess
its
fractions
(LGSF
murine
model
explore
underlying
mechanisms.
Methods
mouse
was
constructed
using
chronic
binge
ethanol
feeding
method.
Biochemical
determinations
AST,
ALT,
TC,
TG,
ADH,
ALDH,
HDL,
LDL,
IL-1β,
IL-6,
TNF-α
were
performed
corresponding
kits.
Histopathological
examination
intestinal
sections
conducted
based
on
H&E
staining.
Lipid
accumulation
hepatocytes
evaluated
by
oil
red
O
Ethanol
metabolism
assessed
determining
activity
ADH
ALDH
enzymes.
Intestinal
barrier
function
analyzed
immunohistochemistry
analysis
ZO-1
occludin
immunofluorescence
epithelial
markers,
Lgr5,
Muc2,
Lyz1.
apoptosis
detected
TUNEL
Mouse
fecal
microbiota
alterations
16S
rRNA
sequencing.
An
vitro
injury
established
developing
TNF-α-induced
3D-cultured
organoids.
In
culture
specific
bacterial
strains
performed.
Results
The
results
showed
significantly
alleviated
mice
through
attenuating
hepatic
inflammation,
improving
steatosis
promoting
metabolism.
Notably,
LGS,
LGSP,
damage
maintained
mice.
protection
generally
more
obvious
than
positive
drug
meltadosine.
Further
promoted
repair
via
Lgr5
+
stem
cell
mediated
regeneration
LGSF,
other
had
better
vitro.
Moreover,
remarkably
gut
dysbiosis
at
least
partially
recovery
alcohol-induced
microbial
changes
induction
groups.
indicated
impact
growth.
but
not
growth
Lactobacillus
.
Similarly,
LGSP
increased
proliferation
Bacteroides
sartorii
,
minimal
effect.
all
Bacillus
coagulans
Bifidobacterium
adolescentis
bifidum
Dubosiella
newyorkensis
no
Conclusions
exerts
regulating
gut-liver
axis,
are
responsible
for
protective
Язык: Английский