The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells DOI Creative Commons
Juanjuan Cheng,

Xinyue Meng,

Daozheng Fang

и другие.

Frontiers in Analytical Science, Год журнала: 2025, Номер 5

Опубликована: Фев. 19, 2025

Zishen Yuzhen Pill (ZYP) is a Chinese herbal product developed by Shenzhen TCM Hospital, which have been frequently used to treat osteoporosis (OP). This study aimed determine the major chemical components of ZYP and its prototype compounds metabolites in rat biological samples, as well explore potential effect ZYP-containing serum MC3T3-E1 cells. UPLC-Q/TOF-MS was identify components. Then, orally administered rat, samples plasma, urine, feces, bile, tissue were collected metabolites. The viability cells evaluated using CCK-8 method after treatment with various concentrations (2%, 4%, 8%) serum. Following serum, activity alkaline phosphatase (ALP) Alizarin red S (ARS) measured, levels Runx2, Opn, Opg Osterix quantified qPCR Western blot analysis. And for RNA-seq Results indicated that total 152 identified ZYP, including flavonoids, iridoid, lignans, triterpene saponins, etc. Furthermore, we detected 70 99 distributed different tissues. In addition, observably promoted osteogenesis increasing ALP ARS activities, up-regulating expression results beneficial effects may be related upregulation mitochondrial oxidative phosphorylation. work provided further support traditional application OP. this can promote pharmacokinetic pharmacological evaluation ZYP.

Язык: Английский

The chemicalome profiling of Zishen Yuzhen Pill in vivo and its promoting effect on osteogenic differentiation of MC3T3-E1 cells DOI Creative Commons
Juanjuan Cheng,

Xinyue Meng,

Daozheng Fang

и другие.

Frontiers in Analytical Science, Год журнала: 2025, Номер 5

Опубликована: Фев. 19, 2025

Zishen Yuzhen Pill (ZYP) is a Chinese herbal product developed by Shenzhen TCM Hospital, which have been frequently used to treat osteoporosis (OP). This study aimed determine the major chemical components of ZYP and its prototype compounds metabolites in rat biological samples, as well explore potential effect ZYP-containing serum MC3T3-E1 cells. UPLC-Q/TOF-MS was identify components. Then, orally administered rat, samples plasma, urine, feces, bile, tissue were collected metabolites. The viability cells evaluated using CCK-8 method after treatment with various concentrations (2%, 4%, 8%) serum. Following serum, activity alkaline phosphatase (ALP) Alizarin red S (ARS) measured, levels Runx2, Opn, Opg Osterix quantified qPCR Western blot analysis. And for RNA-seq Results indicated that total 152 identified ZYP, including flavonoids, iridoid, lignans, triterpene saponins, etc. Furthermore, we detected 70 99 distributed different tissues. In addition, observably promoted osteogenesis increasing ALP ARS activities, up-regulating expression results beneficial effects may be related upregulation mitochondrial oxidative phosphorylation. work provided further support traditional application OP. this can promote pharmacokinetic pharmacological evaluation ZYP.

Язык: Английский

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