PEPSeek-mediated identification of novel epitopes from viral and bacterial pathogens and the impact on host cell immunopeptidomes DOI Creative Commons
John A. Cormican,

Lobna Medfai,

Magdalena Wawrzyniuk

и другие.

Molecular & Cellular Proteomics, Год журнала: 2025, Номер unknown, С. 100937 - 100937

Опубликована: Март 1, 2025

Here, we develop PEPSeek, a web-server based software to allow higher performance in the identification of pathogen-derived epitope candidates detected via mass spectrometry MHC class I immunopeptidomes. We apply it human and mouse cell lines infected with either SARS-CoV-2, Listeria monocytogenes or Chlamydia trachomatis, thereby identifying large number novel antigens epitopes that prove be recognized by CD8+ T cells. In cells, identified antigenic peptide features suggested how processing presentation pathogenic differ between pathogens. The quantitative tools PEPSeek also helped define C. trachomatis infection cycle could impact on landscape host system, likely reflecting metabolic changes occurred

Язык: Английский

PEPSeek-mediated identification of novel epitopes from viral and bacterial pathogens and the impact on host cell immunopeptidomes DOI Creative Commons
John A. Cormican,

Lobna Medfai,

Magdalena Wawrzyniuk

и другие.

Molecular & Cellular Proteomics, Год журнала: 2025, Номер unknown, С. 100937 - 100937

Опубликована: Март 1, 2025

Here, we develop PEPSeek, a web-server based software to allow higher performance in the identification of pathogen-derived epitope candidates detected via mass spectrometry MHC class I immunopeptidomes. We apply it human and mouse cell lines infected with either SARS-CoV-2, Listeria monocytogenes or Chlamydia trachomatis, thereby identifying large number novel antigens epitopes that prove be recognized by CD8+ T cells. In cells, identified antigenic peptide features suggested how processing presentation pathogenic differ between pathogens. The quantitative tools PEPSeek also helped define C. trachomatis infection cycle could impact on landscape host system, likely reflecting metabolic changes occurred

Язык: Английский

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