On subcellular distribution of the zinc finger 469 protein (ZNF469) and observed discrepancy in the localization of endogenous and overexpressed ZNF469

FEBS Open Bio, Год журнала: 2025, Номер unknown

Опубликована: Март 29, 2025

The zinc finger 469 gene (ZNF469) is a single-exon predicted to encode protein of 3953 amino acids. Despite pathogenic ZNF469 variants being associated with Brittle Cornea Syndrome (BCS), relatively little known about beyond its participation in regulating the expression genes encoding extracellular matrix proteins. In this study, we examined and intracellular localization different cell lines. level mRNA varied from low levels HEK293 cells high HeLa primary fibroblasts. Antibodies against reacted among others approximately 400 kDa immunoblot analysis, which was mainly present insoluble fraction cytoplasm. Immunofluorescence analysis interphase showed small cytoplasmic puncta weak nuclear staining. dividing cells, antibodies recognized foci that also stained for proteasomes. transfected observed resembling speckles at midbody during mitosis. overlapping staining live imaging, liquid-like properties were recorded as they changed shape position occasionally fused each other. During stress granule formation, G3BP1. Finally, silico revealed large intrinsically disordered regions multiple complexity domains ZNF469. Our data indicate forms aggregates possibly biomolecular condensates when overexpressed. However, care must be taken analyzing distribution due discrepancy endogenous overexpressed cells.

Язык: Английский

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Proximal relationships of moonlighting proteins in Escherichia coli: A mathematical genomic perspective

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142766 - 142766

Опубликована: Апрель 1, 2025

Язык: Английский

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Mapping dihydropteroate synthase evolvability through identification of a novel evolutionarily critical substructure

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143325 - 143325

Опубликована: Апрель 1, 2025

Язык: Английский

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Ebola virus nucleoprotein interaction with host protein phosphatase-1 regulates its dimerization and capsid formation

Journal of Biological Chemistry, Год журнала: 2025, Номер unknown, С. 108541 - 108541

Опубликована: Апрель 1, 2025

Язык: Английский

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Intrinsic Disorder in Protein Interaction Networks Linking Cancer with Metabolic Diseases

Computational Biology and Chemistry, Год журнала: 2025, Номер 118, С. 108493 - 108493

Опубликована: Апрель 28, 2025

Язык: Английский

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