Unique Fn3‐like biosensor in σI/anti‐σI factors for regulatory expression of major cellulosomal scaffoldins in Pseudobacteroides cellulosolvens DOI Open Access
Sheng Dong, Chao Chen, Jie Li

и другие.

Protein Science, Год журнала: 2024, Номер 33(11)

Опубликована: Окт. 29, 2024

Abstract Lignocellulolytic clostridia employ multiple pairs of alternative σ/anti‐σ (SigI/RsgI) factors to regulate cellulosomal components for substrate‐specific degradation cellulosic biomass. The current model has proposed that RsgIs use a sensor domain bind specific extracellular lignocellulosic and activate cognate SigIs initiate expression corresponding enzyme genes, while scaffoldins can be initiated by several different SigIs. Pseudobacteroides cellulosolvens contains the most complex known cellulosome system highest number SigI–RsgI regulons yet discovered. However, function many RsgI domains their relationship with various types are not fully understood. Here, we report RsgI4 from P. employs C‐terminal module bears distant similarity fibronectin type III (Fn3) serves as domain. Substrate‐binding analysis revealed Fn3‐like represents novel carbohydrate‐binding (CBM) binds wide range polysaccharide types. Structure determination further belongs B group CBMs predicted concave face substrate binding. Promoter sequence genes SigI4 is responsible regulation major rather than enzymes, consistent broad specificity Notably, invariably required regardless type. These findings suggest intricate comprises more elaborate mechanism other bacteria thus expands paradigm regulation.

Язык: Английский

Unique Fn3‐like biosensor in σI/anti‐σI factors for regulatory expression of major cellulosomal scaffoldins in Pseudobacteroides cellulosolvens DOI Open Access
Sheng Dong, Chao Chen, Jie Li

и другие.

Protein Science, Год журнала: 2024, Номер 33(11)

Опубликована: Окт. 29, 2024

Abstract Lignocellulolytic clostridia employ multiple pairs of alternative σ/anti‐σ (SigI/RsgI) factors to regulate cellulosomal components for substrate‐specific degradation cellulosic biomass. The current model has proposed that RsgIs use a sensor domain bind specific extracellular lignocellulosic and activate cognate SigIs initiate expression corresponding enzyme genes, while scaffoldins can be initiated by several different SigIs. Pseudobacteroides cellulosolvens contains the most complex known cellulosome system highest number SigI–RsgI regulons yet discovered. However, function many RsgI domains their relationship with various types are not fully understood. Here, we report RsgI4 from P. employs C‐terminal module bears distant similarity fibronectin type III (Fn3) serves as domain. Substrate‐binding analysis revealed Fn3‐like represents novel carbohydrate‐binding (CBM) binds wide range polysaccharide types. Structure determination further belongs B group CBMs predicted concave face substrate binding. Promoter sequence genes SigI4 is responsible regulation major rather than enzymes, consistent broad specificity Notably, invariably required regardless type. These findings suggest intricate comprises more elaborate mechanism other bacteria thus expands paradigm regulation.

Язык: Английский

Процитировано

1