Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3biomineralization inMicrocystis DOI Creative Commons

Apolline Bruley,

Juliette Gaëtan,

Muriel Gugger

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 7, 2024

Phylogenetically diverse cyanobacteria biomineralize intracellular amorphous calcium carbonate (iACC) inclusions. This includes several genotypes of the Microcystis genus, a potentially toxic, bloom-forming cyanobacterium found worldwide in freshwater ecosystems. While we ignore biological function iACC and molecular mechanisms driving their formation, this process may impact local geochemical cycles and/or be used for bioremediation strategies. Recently, marker gene biomineralization pathway, named ccyA, was discovered. However, calcyanin protein encoded by ccyA remains unknown. Here, based on an RNA-Seq approach, assess expression aeruginosa PCC 7806 during 24 h day/night cycle. The shows clear pattern with maximum transcript abundances second half night. is consistent assumption that related photosynthesis therefore follow cycle as well. Moreover, genes directly co-localized upstream downstream same DNA strand show similar pattern, including cax encoding calcium/proton exchanger domain also present N-terminal region calcyanins many iACC-forming cyanobacteria. suggests they all could part operon, play concerted role formation. Last, other involved carbon concentrating transport to ccyA. Overall, study provides list candidate whose be, future, analyzed biochemistry genetics approaches.

Язык: Английский

Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3 biomineralization in Microcystis DOI Creative Commons

Apolline Bruley,

Juliette Gaëtan,

Muriel Gugger

и другие.

Peer Community Journal, Год журнала: 2025, Номер 5

Опубликована: Фев. 6, 2025

Язык: Английский

Процитировано

0
Diel changes in the expression of a marker gene and candidate genes for intracellular amorphous CaCO3biomineralization inMicrocystis DOI Creative Commons

Apolline Bruley,

Juliette Gaëtan,

Muriel Gugger

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 7, 2024

Phylogenetically diverse cyanobacteria biomineralize intracellular amorphous calcium carbonate (iACC) inclusions. This includes several genotypes of the Microcystis genus, a potentially toxic, bloom-forming cyanobacterium found worldwide in freshwater ecosystems. While we ignore biological function iACC and molecular mechanisms driving their formation, this process may impact local geochemical cycles and/or be used for bioremediation strategies. Recently, marker gene biomineralization pathway, named ccyA, was discovered. However, calcyanin protein encoded by ccyA remains unknown. Here, based on an RNA-Seq approach, assess expression aeruginosa PCC 7806 during 24 h day/night cycle. The shows clear pattern with maximum transcript abundances second half night. is consistent assumption that related photosynthesis therefore follow cycle as well. Moreover, genes directly co-localized upstream downstream same DNA strand show similar pattern, including cax encoding calcium/proton exchanger domain also present N-terminal region calcyanins many iACC-forming cyanobacteria. suggests they all could part operon, play concerted role formation. Last, other involved carbon concentrating transport to ccyA. Overall, study provides list candidate whose be, future, analyzed biochemistry genetics approaches.

Язык: Английский

Процитировано

1