Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 24, 2024
Double-stranded
RNA
(dsRNA)
has
emerged
as
key
player
in
gene
silencing
for
the
past
two
decades.
Tailor-made
dsRNA
is
now
recognized
a
versatile
raw
material,
suitable
wide
range
of
applications
biopesticide
formulations,
including
insect
control
to
pesticide
resistance
management.
The
mechanism
interference
(RNAi)
acts
at
messenger
(mRNA)
level,
utilizing
sequence-dependent
approach
that
makes
it
unique
term
effectiveness
and
specificity
compared
conventional
agrochemicals.
Two
primary
categories
small
RNAs,
known
short
interfering
RNAs
(siRNAs)
microRNAs
(miRNAs),
function
both
somatic
germline
lineages
broad
eukaryotic
species
regulate
endogenous
genes
defend
genome
from
invasive
nucleic
acids.
Furthermore,
application
RNAi
crop
protection
can
be
achieved
by
employing
plant-incorporated
protectants
through
plant
transformation,
but
also
non-transformative
strategies
such
use
formulations
sprayable
direct
agents,
factor
repressors
or
developmental
disruptors.
This
review
explores
agricultural
RNAi,
delving
into
its
successes
pest-insect
considering
broader
potential
managing
pathogens,
nematodes,
pests.
Additionally,
tool
addressing
pesticide-resistant
weeds
insects
reviewed,
along
with
an
evaluation
production
costs
environmental
implications.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 30, 2024
ABSTRACT
The
whitefly,
Bemisia
tabaci
,
is
a
hemipteran
with
haplodiploid
sex
determination
system,
which
provides
natural
experiment
for
examining
genome
function
in
haploid
and
diploid
embryos.
Yet
the
embryogenesis
of
B.
remains
understudied.
Our
previous
work
has
established
possible
role
DNA
methyltransferase
1
(
Dnmt1
)
stability.
In
this
study
we
used
maternal
RNA
interference
(RNAi)
immunohistochemistry
to
complex
dynamics
between
DNMT1
ploidy
during
embryogenesis.
We
found
that
both
have
similar
developmental
timeline
other
holometabolous
insects.
also
that,
like
obligatory
insects,
does
not
affect
timing,
suggesting
factors
play
greater
than
timing.
For
embryos
reduced
expression
loss
disrupts
blastoderm
development
affects
nuclei
morphology
results
suggest
required
development.
EPISTEMUS,
Год журнала:
2024,
Номер
18(37), С. e3702365 - e3702365
Опубликована: Ноя. 5, 2024
Bemisia
tabaci
(Gennadius)
es
un
insecto
polífago
que
ha
invadido
los
campos
de
cultivos
agrícolas
en
todo
el
mundo.
El
control
más
efectivo
sistemas
producción
intensivos
se
basado
uso
insecticidas
químicos,
a
pesar
sus
efectos
adversos.
Ante
esto,
actualmente
emergen
alternativas
con
alta
eficiencia
pueden
ser
empleadas
para
esta
plaga
invernaderos.
Sin
embargo,
la
introducción
una
nueva
estrategia
sistema
intensivo
representa
reto
debe
evaluarse
antes
su
implementación.
En
presente
trabajo
presenta
revisión
del
estado
actual
las
estrategias
efectivas
contra
agrícola
intensiva.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 24, 2024
Double-stranded
RNA
(dsRNA)
has
emerged
as
key
player
in
gene
silencing
for
the
past
two
decades.
Tailor-made
dsRNA
is
now
recognized
a
versatile
raw
material,
suitable
wide
range
of
applications
biopesticide
formulations,
including
insect
control
to
pesticide
resistance
management.
The
mechanism
interference
(RNAi)
acts
at
messenger
(mRNA)
level,
utilizing
sequence-dependent
approach
that
makes
it
unique
term
effectiveness
and
specificity
compared
conventional
agrochemicals.
Two
primary
categories
small
RNAs,
known
short
interfering
RNAs
(siRNAs)
microRNAs
(miRNAs),
function
both
somatic
germline
lineages
broad
eukaryotic
species
regulate
endogenous
genes
defend
genome
from
invasive
nucleic
acids.
Furthermore,
application
RNAi
crop
protection
can
be
achieved
by
employing
plant-incorporated
protectants
through
plant
transformation,
but
also
non-transformative
strategies
such
use
formulations
sprayable
direct
agents,
factor
repressors
or
developmental
disruptors.
This
review
explores
agricultural
RNAi,
delving
into
its
successes
pest-insect
considering
broader
potential
managing
pathogens,
nematodes,
pests.
Additionally,
tool
addressing
pesticide-resistant
weeds
insects
reviewed,
along
with
an
evaluation
production
costs
environmental
implications.
