miR-34-5p, a potential RNA biopesticide for controlling Spodoptera frugiperda by targeting of SfEcR and SfE74

Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер 208, С. 106287 - 106287

Опубликована: Янв. 4, 2025

Язык: Английский

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siRNA self-assembled carboxymethyl chitosan and pymetrozine nucleic pesticides for enhanced control of Sogatella furcifera: Bidirectional transduction and detoxification inhibition

Carbohydrate Polymers, Год журнала: 2025, Номер 354, С. 123328 - 123328

Опубликована: Янв. 28, 2025

Язык: Английский

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Functional Analysis of Lactase Phlorizin Hydrolase in Insect–Plant Coevolution Based on Deglycosylation

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер 73(9), С. 5140 - 5149

Опубликована: Фев. 24, 2025

During coevolution, plants produce numerous toxic secondary metabolites to protect themselves. However, most still serve as food for insects meet their nutritional needs. The evolutionary processes that enable herbivorous resist plant defenses remain largely complex and difficult predict. In this study, lactase phlorizin hydrolase (LPH) was identified the first time in Aphis gossypii. Bioinformatics analysis showed LPH protein belongs glycoside 1 (GH1) family. qPCR transcript level of cotton aphids treated with expression dsAgCYP6CY3 lines significantly reduced by 39.9% at 48 h compared nontransgenic cotton, hydrolytic activities on lactose were 28.7% 20.1%, respectively. vitro enzyme activity experiments demonstrated could hydrolyze four phenolic glycosides. addition, RNA interference (RNAi) insect performance assays silencing affected growth larvae, resulting death larvae exposed These results reveal an scenario whereby herbivores use deglycosylation develop resistance can be exploited protection.

Язык: Английский

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RNAi-mediated knockdown of juvenile hormone acid methyltransferase depresses reproductive performance in female Aethina tumida

Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер 211, С. 106420 - 106420

Опубликована: Апрель 15, 2025

Язык: Английский

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E93 is involved in regulating larval-pupal metamorphosis and the development of the wing and ovary in Helicoverpa armigera, and it shows great potential for RNAi-based pest control

Journal of Integrative Agriculture, Год журнала: 2024, Номер unknown

Опубликована: Июнь 1, 2024

The ecdysone-induced transcription factor E93 in model insects plays multiple roles the insect metamorphosis processes, such as remodeling larval tissues and determining adult tissue formation. knockdown of leads to incomplete metamorphosis, suggesting that is a potential target for pest control. In this study, HaE93 gene cotton bollworm Helicoverpa armigera, polyphagous various commercial crops worldwide, was identified found have high expression egg, prepupal, pupal stages. injection dsHaE93 induced about 60% mortality H. armigera at larval-pupal stage. About 30% surviving showed delayed pupation developed abnormal wings, females reduced ovaries. 90% individuals failed reproduce before they died. results qRT-PCR levels ecdysone primary-response genes, chitin synthesis-related wing ovary development-related genes were armigera. These indicated critical role larva-pupa-adult development cuticle, wing, female by regulating associated genes. Bioassays administered either oral delivery or similar results, which suggested can be used RNAi control

Язык: Английский

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Trophic microRNA: Post‐transcriptional regulation of target genes and larval development impairment in Plutella xylostella upon precursor and mature microRNA ingestion

Insect Molecular Biology, Год журнала: 2024, Номер unknown

Опубликована: Июль 25, 2024

MicroRNAs (miRNAs) are post-transcriptional gene regulators. In the miRNA pathway's cytoplasmic part, is processed from a hairpin-structured precursor to double-stranded (ds) mature RNA and ultimately single-stranded miRNA. insects, ingesting these two ds forms can regulate target expression; this inspired trophic miRNA's use as functional genomics pest management tool. However, systematic studies enabling comparisons of pre- forms, dosages, administration times instar-wise effects on transcripts phenotypes, which help develop method, unavailable due different focuses previous investigations. We investigated impact trophically delivered Px-let-7 lepidopteran Plutella xylostella, compare efficacies its ds-mature forms. Continuous feeding miRNA-supplemented diet suppressed expressions FTZ-F1 E74, ecdysone pathway genes. Both pre-let-7 let-7 similarly downregulated in all four larval instars. Pre-let-7 ingestions decreased mass instar duration increased mortality instars, exhibiting adverse growth development. processing Dicer-1 AGO-1's upregulations upon ingestion denoted systemic spread tissues. The scrambled sequence controls did not affect transcripts, suggesting sequence-specific targeting by hairpin cassette's non-involvement downregulation. This work provides framework for function analyses potentiates utility management.

Язык: Английский

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Dietary cholesterol promotes the growth of mud crabs, Scylla paramamosain by regulating cholesterol metabolism, enhancing ecdysteroid synthesis and molting cascade response

Animal Feed Science and Technology, Год журнала: 2024, Номер unknown, С. 116159 - 116159

Опубликована: Ноя. 1, 2024

Язык: Английский

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