Identification and function of microRNAs in hemipteran pests: A review
Insect Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 18, 2024
Hemiptera
is
one
of
the
most
significant
orders
insect
pests,
including
whiteflies,
true
bugs,
aphids,
planthoppers,
psyllids,
and
so
forth,
which
have
led
to
substantial
economic
losses
in
agricultural
industries
significantly
affected
food
yields
through
their
ability
suck
phloem
sap
crops
transmit
numerous
bacterial
viral
pathogens.
Therefore,
explorations
pest-specific,
eco-friendly
easy-to-adopt
technologies
for
hemipteran
pest
control
are
urgently
needed.
To
best
our
knowledge,
microRNAs
(miRNAs),
endogenous
non-coding
small
RNAs
approximately
22
nucleotides
length,
involved
regulating
gene
expression
via
direct
recognition
binding
3'-untranslated
region
(3'-UTR)
target
messenger
(mRNAs)
or
by
acting
as
a
center
competitive
RNA
(ceRNA)
network
at
post-transcriptional
level.
This
review
systematically
outlines
characterization
functional
investigation
miRNA
biogenesis
pathway
such
aphids
planthoppers.
In
addition,
we
explored
results
sequencing
observations
miRNAs
these
suggest
that
obtained
annotated
high-throughput
technology
bioinformatic
analyses
contribute
molting
development,
fitness,
wing
polyphenism,
symbiont
interactions
insecticide
resistance
pests.
Finally,
summarize
current
advances
propose
framework
future
research
extend
data
address
potential
limitations
application
miRNAs.
Язык: Английский
microRNA Targeting Cytochrome P450 Is Involved in Chlorfenapyr Tolerance in the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)
Insects,
Год журнала:
2025,
Номер
16(5), С. 515 - 515
Опубликована: Май 12, 2025
We
first
measured
the
content
of
chlorfenapyr
and
tralopyril
in
silkworm
larvae
using
HPLC,
revealing
that
can
be
biotransformed
into
silkworms.
Then,
a
differential
transcriptomic
database
small
RNA
was
constructed
through
Illumina
RNA-Sequencing.
qRT-PCR
conducted
to
determine
expression
levels
Bmo-miR-6497-5p
target
CYP450
gene,
significantly
upregulated
L3
24,
48,
72
h
after
they
were
treated
with
chlorfenapyr.
Furthermore,
P450
gene
CYP337A2
downregulated
at
these
time
points.
Dual-luciferase
validation
revealed
luciferase
activity
decreased
bound
CYP337A2.
In
addition,
miRNA
mimics/inhibitor
injection
bioassays
mortality
third
injected
antagomir
increased
exposure
sublethal
concentration
These
results
imply
targets
CYP337A2,
regulating
its
expression.
Also,
silkworms
increase
their
tolerance
by
upregulating
expression,
thereby
inhibiting
biotransformation
toxic
catalyzed
The
present
study
reveals
function
microRNA
improves
understanding
regarding
insecticide
resistance
Lepidopteran
insects.
Язык: Английский
MicroRNA-mediated detoxification network conferring afidopyropen tolerance in Aphis craccivora
Pesticide Biochemistry and Physiology,
Год журнала:
2025,
Номер
unknown, С. 106485 - 106485
Опубликована: Июнь 1, 2025
Язык: Английский
miR-10-5p mediated susceptibility to chlorantraniliprole by targeting for SfGSTe1 in Spodoptera frugiperda (Smith)
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Июнь 5, 2025
Glutathione
S-transferases
(GST)
in
pests
plays
a
critical
role
insecticide
resistance.
In
this
study,
SfGSTe1
expression
significant
upregulation
under
chlorantraniliprole
exposure,
susceptibility
improvement
after
injected
dsSfGSTe1
and
miR-10-5p
target
located
the
3'
UTR
of
conducted
by
luciferase
reporter
assays
were
observed
Spodoptera
frugiperda
(Smith).
Injected
agomir
reduced
significantly
abundance,
together
with
increased
susceptibility,
while
antagomir
improved
abundance
thus
decreased
larvae
S.
frugiperda.
We
revealed
that
mRNA
stability
preventing
its
degradation
could
have
been
induced
resistance
to
chlorantraniliprole.
This
finding
would
help
study
mechanism
miRNAs
mediating
pests.
Язык: Английский
Overexpression of SmUGGT1 Confers Imidacloprid Resistance to Sitobion miscanthi (Takahashi)
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(32), С. 17824 - 17833
Опубликована: Авг. 1, 2024
Sitobion
miscanthi,
the
main
species
of
wheat
aphids,
is
one
kind
harmful
pest.
Chemical
insecticides
are
important
agrochemical
products
to
effectively
control
aphids.
However,
broad
application
has
led
serious
resistance
pests
several
insecticides,
and
understanding
insecticide
mechanisms
critical
for
integrated
pest
management.
In
this
study,
SmUGGT1,
a
new
uridine
diphosphate
(UDP)-glycosyltransferase
(UGT)
gene,
was
cloned
more
strongly
expressed
in
SM-R
(the
resistant
strain
imidacloprid)
than
SM-S
susceptible
imidacloprid).
The
increased
susceptibility
imidacloprid
observed
after
silencing
indicating
that
it
can
be
related
imidacloprid.
Subsequently,
SmUGGT1
regulated
post-transcriptionally
coding
sequences
(CDs)
by
miR-81
verified
involved
S.
miscanthi.
This
finding
crucial
roles
UGT
management
pests.
Язык: Английский
Structural Derivatives of β-Asarone from Acorus calamus Linn. as Insecticide Candidates and the Insecticidal Mechanism Against Small Brown Planthopper
Agronomy,
Год журнала:
2024,
Номер
14(10), С. 2420 - 2420
Опубликована: Окт. 18, 2024
The
small
brown
planthopper
(SBPH),
Laodelphax
striatellus
(Fallén)
(Hemiptera:
Delphacidae),
is
an
increasing
threat
to
Gramineae
crops,
posing
significant
risks
both
the
environment
and
food
safety.
β-asarone,
as
a
promising
green
alternative
chemical
insecticides,
possesses
wide
application
prospects
in
crop
protection
field.
To
enhance
insecticidal
activity
of
series
derivatives
were
prepared
through
active
substructure
splicing
strategy,
their
activities
against
SBPH
evaluated.
Among
7
commercial
compounds
with
structures
similar
β-asarone
12
structural
compound
10,
which
incorporates
2-chloropyridine
functional
group
from
flupyrimin,
exhibited
most
potent
SBPH,
8.31-fold
increase
compared
β-asarone.
Furthermore,
transcriptome
analysis
showed
that
among
selected
genes
may
play
important
roles
activity,
ABC
transporter
gene,
MDR49,
was
significantly
down-regulated.
MDR49
highly
expressed
4th-instar
nymphs,
highest
expression
level
fat
body,
midgut,
abdomen.
RNA
interference
(RNAi)
reduced
susceptibility
10
revealed
be
candidate
target
10.
Additionally,
spectrum
excellent
efficacy
Bemisia
tabaci
(Gennadius)
Aleyrodidae)
Tetranychus
cinnabarinus
(Boisduval)
(Acarina:
Tetranychidae).
This
study
indicates
could
further
developed
novel
eco-friendly
pesticide.
Язык: Английский