Insects,
Год журнала:
2024,
Номер
15(8), С. 569 - 569
Опубликована: Июль 26, 2024
Diptera
and
Lepidoptera
species
have
the
highest
levels
of
insecticide
resistance,
mechanism
drug
resistance
has
been
studied
in
detoxification
metabolism
genes
such
as
P450,
GST,
EST,
ABC.
Since
Bactrocera
dorsalis
are
resistant
to
a
variety
chemicals,
pattern
investigated
from
aspects
genes,
enzymes,
intestinal
symbiotic
bacteria,
synergists
world.
In
this
study,
51
149
were
annotated
Suppression
Subtractive
Hybridization
(SSH)
library,
12
candidate
related
beta-cypermethrin
screened
quantitatively
expressed
library.
Two
found
be
upregulated
egg
stage,
three
larval
one
gene
pupal
five
adult
four
midgut
malacca
ducts
midgut.
The
expression
cyp6g1,
cyp6a22,
GST-Epsilon9,
Trypsin-4
was
strains,
with
most
obvious
upregulation
occurring
Malpighian
tubules.
These
results
provide
new
insights
into
study
pesticide
quarantine
insects.
Insects,
Год журнала:
2025,
Номер
16(5), С. 528 - 528
Опубликована: Май 15, 2025
The
large
growth
in
the
global
population
requires
new
solutions
for
control
of
harmful
insects
that
compete
our
food.
Changing
regulatory
requirements
and
public
perception,
together
with
continuous
evolution
resistance
to
conventional
insecticides,
also
require,
addition
innovative
molecules
different
modes
action,
non-chemical
strategies
can
help
maintain
efficient
integrated
pest
management
programs.
last
30
years
have
inaugurated
a
era
characterised
by
discovery
mechanisms
action
chemical
families.
Although
European
programs
promote
green
deal
crop
protection
sector,
existing
thorough
regulations
slow
down
its
spread
adoption
products.
In
light
these
changes,
this
review
will
describe
more
detail
dynamics
registration
insecticides
difficulties
agrochemical
industries
encounter.
Subsequently,
alternative
based
on
natural
substances
origin,
entomopathogenic
microorganisms,
semiochemical
semiophysical
compounds,
classical
augmentative
biological
be
described.
advantages
illustrated
constrains
their
diffusion
commercialisation.
Finally,
main
biotechnological
discoveries
described,
from
transgenic
plants
symbiotic
control,
genetic
and,
recently,
insect
genomic
transformation
or
RNAi.
These
biotechnologies
revolutionise
sector
despite
some
related
restrictions
present
countries.
Insects,
Год журнала:
2024,
Номер
15(8), С. 595 - 595
Опубликована: Авг. 5, 2024
Detoxification
genes
are
crucial
to
insect
resistance
against
chemical
pesticides,
yet
their
expression
may
be
altered
by
exposure
biopesticides
such
as
spores
and
insecticidal
proteins
of
Bacillus
thuringiensis
(Bt).
Increased
enzymatic
levels
selected
detoxification
genes,
including
glutathione
S-transferase
(GST),
cytochrome
P450
(CYP450),
carboxylesterase
(CarE),
were
detected
in
chlorantraniliprole
(CAP)-resistant
strains
the
diamondback
moth
(DBM,
Plutella
xylostella)
from
China
when
compared
a
reference
susceptible
strain.
These
CAP-resistant
DBM
displayed
distinct
patterns
GST
1,
CYP6B7,
CarE-6
after
treatment
with
CAP
Bt
pesticide
(Bt-G033).
In
particular,
gene
analysis
demonstrated
significant
upregulation
CYP6B7
response
treatment,
while
same
was
downregulated
following
Bt-G033
treatment.
Downregulation
using
RNAi
resulted
increased
susceptibility
resistant
strains,
suggesting
role
this
phenotype.
However,
pretreatment
sublethal
dose
inducing
downregulation
did
not
significantly
increase
potency
strains.
results
identify
involved
metabolic
demonstrate
how
is
affected
Bt-G033.
