Method for Screening Sodium Cyanoborohydride for Free Cyanide Content and Its Impact on Bioconjugation Chemistry DOI Creative Commons
Jarrod Cohen, Adam J. DiCaprio, Jian He

и другие.

Bioconjugate Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Sodium cyanoborohydride (CBH) is commonly used as a mild reducing agent in the reductive amination of aldehydes and free amines. Within pharmaceutical industry, this reaction employed bioconjugation proteins peptides. Free cyanide species such HCN NaCN are known residual impurities CBH that can contribute to formation undesired side products including cyanoamines cyanohydrins. In commercial processes, potential for bound cyanated requires an analytical control strategy monitor mitigate any risk human health. Given these concerns, minimization utmost priority be achieved through robust quantitative screening starting materials cyanide. Alternative mitigation strategies purification containing pure less effective due minor chemical differences between expected product species. Herein, we present simple chromatographic assay quantitation raw material sodium cyanoborohydride. Method development, robustness evaluation, scientific soundness assessment reported with excellent linearity, accuracy, precision, specificity. Additionally, method was applied evaluation supplied from 10 sources, none which report specification within their certificate analysis. The measured vendors ranged 8 80 mM concentration, thereby confirming value materials. Finally, demonstrate impact on model ornithine glyceraldehyde.

Язык: Английский

Advance in peptide-based drug development: delivery platforms, therapeutics and vaccines DOI Creative Commons
Wen‐Jing Xiao, Wenjie Jiang, Zheng Chen

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)

Опубликована: Март 5, 2025

The successful approval of peptide-based drugs can be attributed to a collaborative effort across multiple disciplines. integration novel drug design and synthesis techniques, display library technology, delivery systems, bioengineering advancements, artificial intelligence have significantly expedited the development groundbreaking drugs, effectively addressing obstacles associated with their character, such as rapid clearance degradation, necessitating subcutaneous injection leading increasing patient discomfort, ultimately advancing translational research efforts. Peptides are presently employed in management diagnosis diverse array medical conditions, diabetes mellitus, weight loss, oncology, rare diseases, additionally garnering interest facilitating targeted platforms advancement vaccines. This paper provides an overview present market clinical trial progress therapeutics, platforms, It examines key areas through literature analysis emphasizes structural modification principles well recent advancements screening, design, technologies. accelerated including peptide-drug complexes, new vaccines, innovative diagnostic reagents, has potential promote era precise customization disease therapeutic schedule.

Язык: Английский

Процитировано

1

Tumor-Homing Peptides as Crucial Component of Magnetic-Based Delivery Systems: Recent Developments and Pharmacoeconomical Perspective DOI Open Access
Sylwia Milewska, Anna Sadowska,

Natalia Stefaniuk

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 6219 - 6219

Опубликована: Июнь 5, 2024

According to data from the World Health Organization (WHO), cancer is considered be one of leading causes death worldwide, and new therapeutic approaches, especially improved novel treatment regimens, are in high demand. Considering that many chemotherapeutic drugs tend have poor pharmacokinetic profiles, including rapid clearance limited on-site accumulation, a combined approach with tumor-homing peptide (THP)-functionalized magnetic nanoparticles could lead remarkable improvements. This confirmed by an increasing number papers this field, showing on-target functionalization improves their penetration properties ensures tumor-specific binding, which results increased clinical response. review aims highlight potential applications THPs combination carriers across various fields, pharmacoeconomic perspective.

Язык: Английский

Процитировано

6

Innovative design concepts in tumor-targeting peptide-drug conjugates: Insights into emerging applications DOI Creative Commons
Ruirong Tan,

Zhenya Yang,

Xie Jun

и другие.

Chinese Medical Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Abstract Peptide-drug conjugates (PDCs) have emerged as a promising strategy in cancer therapy, offering improved therapeutic efficacy and reduced toxicity. Compared to antibody-drug (ADCs) small molecule-drug (SMDCs), PDCs possess distinct advantages, such lower immunogenicity, tumor penetration, simpler synthesis. This review discusses the latest advancements PDC design, including novel peptide targeting mechanisms, linker selection, formulation improvements for increased stability. Additionally, it explores expanding clinical applications of examines their limitations. The aim this is provide comprehensive overview current progress outline future directions role treatment.

Язык: Английский

Процитировано

0

Unlocking the Potential: Receptor-Mediated Targeted Drug Delivery in Cancer Therapy DOI

Balaji Palanisamy,

Abul Kalam Azad Mandal

Pathology - Research and Practice, Год журнала: 2025, Номер unknown, С. 155955 - 155955

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Development of a blue innovative antioxidant, biodegradable packaging material with Clitoria ternatea L. flos DOI Creative Commons

Marcin Szymański,

Mikołaj Pawlik,

Renata Dobrucka

и другие.

Food Bioscience, Год журнала: 2024, Номер 62, С. 105047 - 105047

Опубликована: Сен. 3, 2024

Today, the packaging industry, and in particular plastic are going to experience a number of challenges as result provisions included directive on reducing environmental impact materials. Therefore, much attention is being paid bio-based based natural biodegradable polymers. Focus placed additives with antioxidant properties materials, eliminating risks associated migration chemical, often harmful substances into food. present study, we obtained blue material flowers Clitoria ternatea L., which additionally safe against selected plants invertebrates. Furthermore, observed that addition matcha improved barrier tested films. Samples CM (cellulose/matcha) CZM (cellulose/gelatin/matcha) showed WVTR values 14.44 [g/m2ˑd] 9.34 [g/m2ˑd], respectively. Antioxidant activity testing by both DPPH radical FRAP methods film containing extract. The extract alone had highest (DPPH IC50 = 1.44 mg/ml; IT0.5 0.64 mg/ml). stronger than film. samples without were characterised TS 13.54 [MPa] 22.24 [MPa], In contrast, their elongation at break was 74.87 [%] 41.04 [%]. weakest mechanical properties: CZM. cellulose/matcha (CM) EAB 5.11 5.05 [%], Whereas cellulose/gelatin/matcha (CZM) 6.45 1.54 Application high concentration resulted separation phase leading weakening change optical performance matcha-containing films this study due colour matcha, an effect presence dried flowers. has marketing role it can make product 'stand out' shop shelf.

Язык: Английский

Процитировано

4

Method for Screening Sodium Cyanoborohydride for Free Cyanide Content and Its Impact on Bioconjugation Chemistry DOI Creative Commons
Jarrod Cohen, Adam J. DiCaprio, Jian He

и другие.

Bioconjugate Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Sodium cyanoborohydride (CBH) is commonly used as a mild reducing agent in the reductive amination of aldehydes and free amines. Within pharmaceutical industry, this reaction employed bioconjugation proteins peptides. Free cyanide species such HCN NaCN are known residual impurities CBH that can contribute to formation undesired side products including cyanoamines cyanohydrins. In commercial processes, potential for bound cyanated requires an analytical control strategy monitor mitigate any risk human health. Given these concerns, minimization utmost priority be achieved through robust quantitative screening starting materials cyanide. Alternative mitigation strategies purification containing pure less effective due minor chemical differences between expected product species. Herein, we present simple chromatographic assay quantitation raw material sodium cyanoborohydride. Method development, robustness evaluation, scientific soundness assessment reported with excellent linearity, accuracy, precision, specificity. Additionally, method was applied evaluation supplied from 10 sources, none which report specification within their certificate analysis. The measured vendors ranged 8 80 mM concentration, thereby confirming value materials. Finally, demonstrate impact on model ornithine glyceraldehyde.

Язык: Английский

Процитировано

0