Microbial Cell Factories,
Год журнала:
2022,
Номер
21(1)
Опубликована: Окт. 10, 2022
Abstract
Background
Glucoside
natural
products
have
been
showing
great
medicinal
values
and
potentials.
However,
the
production
of
glucosides
by
plant
extraction,
chemical
synthesis,
traditional
biotransformation
is
insufficient
to
meet
fast-growing
pharmaceutical
demands.
Microbial
synthetic
biology
offers
promising
strategies
for
synthesis
diversification
glycosides.
Results
In
this
study,
two
efficient
UDP-glucosyltransferases
(UGTs)
(UGT85A1
RrUGT3)
origin,
that
are
capable
recognizing
phenolic
aglycons,
characterized
in
vitro.
The
UGTs
show
complementary
regioselectivity
towards
alcoholic
hydroxyl
groups
on
substrates.
By
combining
a
developed
alkylphenol
bio-oxidation
system
these
UGTs,
twenty-four
enzymatically
synthesized
from
readily
accessible
Based
glycosylation
systems,
number
microbial
cell
factories
constructed
applied
biotransformation,
giving
rise
variety
plant-like
O
-glucosides.
Remarkably,
several
unnatural
-glucosides
prepared
demonstrate
better
prolyl
endopeptidase
inhibitory
and/or
anti-inflammatory
activities
than
those
clinically
used
glucosidic
drugs
including
gastrodin,
salidroside
helicid.
Furthermore,
also
able
catalyze
formation
N
-
S
-glucosidic
bonds
produce
Conclusions
Two
highly
UGT85A1
RrUGT3,
with
distinct
were
study.
A
group
efficiently
cell-based
using
UGTs.
Many
exhibited
PEP
or
plant-origin
glucoside
drugs,
significant
potentials
new
drug
development.
Plants,
Год журнала:
2025,
Номер
14(7), С. 1000 - 1000
Опубликована: Март 22, 2025
This
study
characterizes
the
metabolomic
profiles
of
three
reference
apricot
cultivars
('Bergeron',
'Currot',
and
'Goldrich')
using
1H
NMR
spectroscopy
untargeted
UPLC-QToF
MS/MS
to
support
plant
breeding
by
correlating
data
with
fruit
phenotyping.
The
primary
objective
was
identify
quantify
key
metabolites
influencing
quality
from
a
nutraceutical
perspective.
analysis
revealed
significant
differences
in
secondary
among
cultivars.
'Bergeron'
'Goldrich'
exhibited
higher
concentrations
organic
acids
(109
mg/g
malate
202
citrate
'Goldrich'),
flavonoids
such
as
epicatechin
(0.44
0.79
mg/g,
respectively),
sucrose
(464
546
mg/g),
contributing
their
acidity-to-sugar
balance.
Conversely,
'Currot'
showed
levels
amino
(24.44
asparagine)
sugars,
particularly
fructose
glucose
(79
180
enhancing
its
characteristic
sweetness.
These
findings
suggest
that
profiling
can
provide
valuable
insights
into
biochemical
pathways
underlying
traits,
aiding
selection
desirable
characteristics.
integration
phenotyping
offers
comprehensive
approach
understanding
diversity,
crucial
for
high-quality,
nutritionally
enriched
fruits
meet
market
demands.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2024,
Номер
24(1)
Опубликована: Дек. 15, 2024
Abstract
The
increasing
prevalence
of
metabolic
diseases
and
the
global
drive
toward
achieving
Sustainable
Development
Goals
(SDGs)
underscore
need
for
sustainable,
nutrient‐dense
foods.
Soybeans
(
Glycine
max
),
a
critical
crop,
offer
promising
solutions;
however,
their
predominant
use
as
animal
feed
raises
concerns
regarding
food
security
environmental
sustainability.
Fermented
soy
products—including
tempeh,
natto,
miso—are
rich
in
bioactive
compounds
such
peptides
isoflavones,
which
potential
therapeutic
effects
hold
cultural
nutritional
significance.
These
fermented
products
provide
profiles
with
unique
health‐promoting
properties.
This
review
critically
examines
generated
through
fermentation,
focusing
on
bioconversion
pathways
gastrointestinal
tract
implications
human
health.
Recent
consumer
demand
novel
ingredients
additional
biological
benefits
has
fueled
research
into
advanced
extraction
techniques,
enhancing
functional
applications
from
these
soy‐based
products.
further
explores
innovations
methods
that
improve
yield
sustainability,
reinforcing
applicability
interventions.
originality
this
lies
its
in‐depth
exploration
alongside
latest
sustainable
designed
to
optimize
use.
Future
should
aim
refine
fermentation
processes,
investigate
synergistic
microbial
interactions,
develop
environmentally
production
methods.
efforts
have
position
essential
contributors
systems,
addressing
both
public
health
needs.
Microbial Cell Factories,
Год журнала:
2022,
Номер
21(1)
Опубликована: Окт. 10, 2022
Abstract
Background
Glucoside
natural
products
have
been
showing
great
medicinal
values
and
potentials.
However,
the
production
of
glucosides
by
plant
extraction,
chemical
synthesis,
traditional
biotransformation
is
insufficient
to
meet
fast-growing
pharmaceutical
demands.
Microbial
synthetic
biology
offers
promising
strategies
for
synthesis
diversification
glycosides.
Results
In
this
study,
two
efficient
UDP-glucosyltransferases
(UGTs)
(UGT85A1
RrUGT3)
origin,
that
are
capable
recognizing
phenolic
aglycons,
characterized
in
vitro.
The
UGTs
show
complementary
regioselectivity
towards
alcoholic
hydroxyl
groups
on
substrates.
By
combining
a
developed
alkylphenol
bio-oxidation
system
these
UGTs,
twenty-four
enzymatically
synthesized
from
readily
accessible
Based
glycosylation
systems,
number
microbial
cell
factories
constructed
applied
biotransformation,
giving
rise
variety
plant-like
O
-glucosides.
Remarkably,
several
unnatural
-glucosides
prepared
demonstrate
better
prolyl
endopeptidase
inhibitory
and/or
anti-inflammatory
activities
than
those
clinically
used
glucosidic
drugs
including
gastrodin,
salidroside
helicid.
Furthermore,
also
able
catalyze
formation
N
-
S
-glucosidic
bonds
produce
Conclusions
Two
highly
UGT85A1
RrUGT3,
with
distinct
were
study.
A
group
efficiently
cell-based
using
UGTs.
Many
exhibited
PEP
or
plant-origin
glucoside
drugs,
significant
potentials
new
drug
development.
