Indomethacin Partially Alleviates Depressive‐Like Behaviors in Maternally Separated Mice: Targeting Neuroinflammatory Response in the Hippocampus DOI

Milad Nemati Khoei,

Narges Alinaghipour,

Elham Saghaei

и другие.

Journal of Biochemical and Molecular Toxicology, Год журнала: 2025, Номер 39(5)

Опубликована: Май 1, 2025

ABSTRACT Experiencing early‐life stress (ELS) plays a fundamental role in the development of mood disorders like depression adulthood. Neuro‐immune response brain is coupled with depressive‐like behaviors following maternal separation (MS) stress. Indomethacin (IND) nonsteroidal anti‐inflammatory drug. This study aimed to assess antidepressant‐like effect IND mouse model MS stress, focusing on its potential neuro‐inflammatory hippocampus. To do this, male NMRI mice were treated at doses 5, 10, and 20 mg/kg, saline (10 mL/kg) or fluoxetine (30 mg/kg). Drugs injected intraperitoneally for 7 days. Behavioral tests including forced swimming test, splash open field test performed. Quantitative reverse transcription‐PCR was used evaluation gene expression inflammatory mediators iNOS , TLR4 NLRP3 TNF‐α IL‐1β Results showed that provoked behavior as observed by decrease grooming activity time an increase immobility test. Depressive‐like neuroinflammation attenuated well decreased neuroinflammation‐related hippocampus mice. In conclusion, results IND, least part, via alleviation hippocampus, exerted effects

Язык: Английский

Urolithin A alleviates schizophrenic-like behaviors and cognitive impairment in rats through modulation of neuroinflammation, neurogenesis, and synaptic plasticity DOI Creative Commons
Zhengyuan Huang,

Zhongyu Ren,

Sanwang Wang

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 26, 2025

Cognitive impairment in schizophrenia occurs the early stages of disease and is closely associated with prognosis. Alleviation cognitive faces major challenges owing to lack preventive therapeutic drugs that are novel effective. Urolithin A (UA) a gut microbial metabolite ellagic acid has demonstrated neuroprotective effects multiple neurological models. Nonetheless, neuromodulatory role UA yet be elucidated. Wistar rat pups were separated from their mothers for 24 h on postnatal days (PNDs) 9–10 establish an early-life stress model. The pretreated at different administration times (2, 4, 6 weeks) doses (50, 100, 150 mg/kg) adolescence (PND29). Behavioral tests performed after end administration. Subsequently, hippocampal samples collected histopathological molecular evaluations. Male offspring rats subjected maternal separation exhibited increased sensitivity prepulse inhibition impairment, accompanied by severe neuroinflammation impaired neurogenesis. However, attenuated separation-induced deficits impairments restored neurogenesis dose-dependent manner. Furthermore, pretreatment preserved dendritic spine density, synapses, presynaptic vesicles. In addition, it exerted anti-inflammatory inhibiting microglial activation expression proinflammatory cytokines tumor necrosis factor-α, interleukin-6, interleukin-1β. Potential mechanisms included upregulation brain-derived neurotrophic factor protein extracellular signal-regulated kinase signaling pathway. This study first preclinical evaluation schizophrenia. findings suggest changes function linked driven interaction among neuroinflammation, neurogenesis, synaptic plasticity potential reverse these processes. These observations provide evidence future clinical trials as dietary supplement preventing

Язык: Английский

Процитировано

0

Indomethacin Partially Alleviates Depressive‐Like Behaviors in Maternally Separated Mice: Targeting Neuroinflammatory Response in the Hippocampus DOI

Milad Nemati Khoei,

Narges Alinaghipour,

Elham Saghaei

и другие.

Journal of Biochemical and Molecular Toxicology, Год журнала: 2025, Номер 39(5)

Опубликована: Май 1, 2025

ABSTRACT Experiencing early‐life stress (ELS) plays a fundamental role in the development of mood disorders like depression adulthood. Neuro‐immune response brain is coupled with depressive‐like behaviors following maternal separation (MS) stress. Indomethacin (IND) nonsteroidal anti‐inflammatory drug. This study aimed to assess antidepressant‐like effect IND mouse model MS stress, focusing on its potential neuro‐inflammatory hippocampus. To do this, male NMRI mice were treated at doses 5, 10, and 20 mg/kg, saline (10 mL/kg) or fluoxetine (30 mg/kg). Drugs injected intraperitoneally for 7 days. Behavioral tests including forced swimming test, splash open field test performed. Quantitative reverse transcription‐PCR was used evaluation gene expression inflammatory mediators iNOS , TLR4 NLRP3 TNF‐α IL‐1β Results showed that provoked behavior as observed by decrease grooming activity time an increase immobility test. Depressive‐like neuroinflammation attenuated well decreased neuroinflammation‐related hippocampus mice. In conclusion, results IND, least part, via alleviation hippocampus, exerted effects

Язык: Английский

Процитировано

0