LC-MS/MS characterization of SOBERANA®02, a receptor binding domain-tetanus toxoid conjugate vaccine against SARS-CoV-2 DOI Creative Commons
Olivia Martínez Armenteros, Darielys Santana-Mederos, Satomy Pousa

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 30, 2024

Abstract SOBERANA ® 02 is a safe and effective conjugate vaccine against SARS-CoV-2, produced using the maleimide-thiol chemistry. In this vaccine, Cys 538 in recombinant receptor binding domain (RBD) of SARS-CoV-2 linked, through thiosuccinimide linker, to lysine residues tetanus toxoid (TT) preparation. LC-MS/MS analysis revealed that TT complex mixture proteins, similar other TTs where detoxified neurotoxin (d-TeNT) has been shown be most abundant protein (30-56%), regardless quantification method used. The fifteen proteins account for approximately 78% total proteins. activation process showed 102 out 107 d-TeNT incorporated maleimide group. contrast, when tryptic peptides isolated by Ni 2+ -NTA affinity chromatography, were analyzed LC-MS/MS, only 22 Lys cross-linked RBD C -terminal peptide ( CVNF 541 -HHHHHH), probably due steric hindrance. Twelve eighteen conjugation sites assigned based on identification linear carrying conjugated (Δm = +1454.58 Da or Δm +1472.59 Da) with stabilized linker forms, respectively. Eight coincidently both strategies. assignment was manually validated observed regularities (z≥3+, XIC, immonium ions, specific fragment ions) not considered software (PEAKS, Kojak pLink2). also conjugated, but lesser extent, ten low abundance carrier To our knowledge, work first report site TT-based vaccine.

Язык: Английский

Characterization by LC-MS/MS analysis of KLH vaccine conjugated with a tick antigen peptide DOI
Satomy Pousa, Pablo E. Ramos‐Bermúdez, Vladimir Besada

и другие.

The Analyst, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Keyhole limpet haemocyanins (KLH1 and KLH2) from Megathura crenulata , are multi-subunit oxygen-carrying metalloproteins of approximately 3900 amino acids, that widely used as carrier proteins in conjugate vaccines immunotherapy.

Язык: Английский

Процитировано

0
LC-MS/MS characterization of SOBERANA®02, a receptor binding domain-tetanus toxoid conjugate vaccine against SARS-CoV-2 DOI Creative Commons
Olivia Martínez Armenteros, Darielys Santana-Mederos, Satomy Pousa

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 30, 2024

Abstract SOBERANA ® 02 is a safe and effective conjugate vaccine against SARS-CoV-2, produced using the maleimide-thiol chemistry. In this vaccine, Cys 538 in recombinant receptor binding domain (RBD) of SARS-CoV-2 linked, through thiosuccinimide linker, to lysine residues tetanus toxoid (TT) preparation. LC-MS/MS analysis revealed that TT complex mixture proteins, similar other TTs where detoxified neurotoxin (d-TeNT) has been shown be most abundant protein (30-56%), regardless quantification method used. The fifteen proteins account for approximately 78% total proteins. activation process showed 102 out 107 d-TeNT incorporated maleimide group. contrast, when tryptic peptides isolated by Ni 2+ -NTA affinity chromatography, were analyzed LC-MS/MS, only 22 Lys cross-linked RBD C -terminal peptide ( CVNF 541 -HHHHHH), probably due steric hindrance. Twelve eighteen conjugation sites assigned based on identification linear carrying conjugated (Δm = +1454.58 Da or Δm +1472.59 Da) with stabilized linker forms, respectively. Eight coincidently both strategies. assignment was manually validated observed regularities (z≥3+, XIC, immonium ions, specific fragment ions) not considered software (PEAKS, Kojak pLink2). also conjugated, but lesser extent, ten low abundance carrier To our knowledge, work first report site TT-based vaccine.

Язык: Английский

Процитировано

0