The Biosynthesis and Functions of Polyamines in the Interaction of Plant Growth-Promoting Rhizobacteria with Plants

Plants, Год журнала: 2023, Номер 12(14), С. 2671 - 2671

Опубликована: Июль 17, 2023

Plant growth-promoting rhizobacteria (PGPR) are members of the plant rhizomicrobiome that enhance growth and stress resistance by increasing nutrient availability to plant, producing phytohormones or other secondary metabolites, stimulating defense responses against abiotic stresses pathogens, fixing nitrogen. The use PGPR increase crop yield with minimal environmental impact is a sustainable readily applicable replacement for portion chemical fertilizer pesticides required high-yielding varieties. Increased health productivity have long been gained applying as commercial inoculants crops, although uneven results. establishment plant–PGPR relationships requires exchange signals nutrients between partners, polyamines (PAs) an important class compounds act physiological effectors signal molecules in plant–microbe interactions. In this review, we focus on role PAs interactions plants. We describe basic ecology production function them plants which they interact. examine metabolism roles individually during their interaction one another. Lastly, some directions future research.

Язык: Английский

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GENOTYPE-BY-GENOTYPE INTERKINGDOM CROSS-TALK BETWEEN SYMBIOTIC NITROGEN FIXING SINORHIZOBIUM MELILOTI STRAINS AND TRICHODERMA SPECIES

Microbiological Research, Год журнала: 2024, Номер 285, С. 127768 - 127768

Опубликована: Май 18, 2024

In the understanding of molecular interaction between plants and their microbiome, a key point is to identify simplified models microbiome including relevant bacterial fungal partners which could also be effective in plant growth promotion. Here, as proof-of-concept, we aim possible interactions symbiotic nitrogen-fixing rhizobia soil fungi (Trichoderma spp.), hence shed light on synergistic roles rhizospheric have biology nitrogen fixation bacteria. We selected 4 strains model rhizobium Sinorhizobium meliloti Trichoderma species (T. velutinum, T. tomentosum, gamsii harzianum). an experimental scheme ×4 x combinations, investigated physiological transcriptomic responses elicited by spent media, well media effects rhizobia-host legume (alfalfa, Medicago sativa L.) symbiosis. Fungal had large rhizobia, specific for each rhizobial combination, indicating generalized genotype interaction, synergistic, neutral antagonistic alfalfa phenotypes. Differential expression high number genes was shown with up 25% total differentially expressed upon treatment cultures media. Percentages over type changed according both strain. To support hypothesis nested Likelihood Ratio Test indicated that considering fungus-rhizobium explained 23.4% genes. Our results provide insights into involving fungi, highlighting panoply genotypic (fungus, rhizobium, host plant) may concur

Язык: Английский

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Rhizobacteria inoculation of plants for reducing insect pests: A meta-analysis on insect behaviour and fitness

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 18, 2024

Abstract Microbial communities in the plant rhizosphere—the soil region closely associated with roots— play critical roles shaping growth, defence, fitness, and ecosystem processes. Inoculation of plants specific rhizobacteria strains has shown promising potential for increasing crop yields. Rhizobacteria can also induce defences, resulting reduced insect growth reproduction, manipulate biochemistry to alter host-choice recruit natural enemies insects. We present a meta-analysis examining impact inoculation on fitness behaviour. Our findings indicate that generally reduces herbivore host choice behaviours. However, effect sizes varied significantly depending type (chewing versus sucking), host, rhizobacterial species. Bacillus spp. showed stronger effects than commonly studied Pseudomonas notably traits such as choice, leaf consumption, survival, reproduction chewing herbivores, while primarily impacting sucking herbivores by reducing reproduction. Single-strain inoculants tended perform better, especially suggesting strain incompatibility issues multi-strain inoculants. Furthermore, field trials less reduction compared experiments under controlled conditions, possibly due diversity environmental factors affecting inoculant persistence. Despite, very limited experimental data, studies observed attract parasitoid wasps predators plants. These results underscore need considering broader interactions when developing effective rhizobacteria-based pest management strategies. Understanding generalist rhizosphere aid synthetic microbial broad protective functions across various environments.

Язык: Английский

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The Biosynthesis and Functions of Polyamines in the Interaction of Plant Growth-Promoting Rhizobacteria with Plants

Опубликована: Июнь 21, 2023

Plant growth-promoting rhizobacteria (PGPR) are members of the plant rhizomicrobiome that enhance growth and stress resistance by increasing nutrient availability to plant, producing phytohormones or other secondary metabolites, stimulating defense responses against abiotic stresses pathogens, fixing nitrogen. The use PGPR increase crop yield with minimal environmental impact is a sustainable readily applicable replacement for portion chemical fertilizer pesticides required high-yielding varieties. Increased health productivity have long been gained applying as commercial inoculants crops, although uneven results. establishment plant-PGPR relationships requires exchange signals nutrients between partners, polyamines (PAs) an important class compounds act physiological effectors signal molecules in plant-microbe interactions. In this review we focus on role PAs interactions plants. We describe basic ecology production function them plants which they interact. examine metabolism roles individually during their interaction one another. Lastly, some directions future research.

Язык: Английский

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Cereals can trap endophytic bacteria when grown ex-situ in harsh soils

FEMS Microbiology Ecology, Год журнала: 2024, Номер 100(5)

Опубликована: Март 27, 2024

Abstract Microbial communities associated with plants growing in harsh conditions, including salinity and water deficiency, have developed adaptive features which permit them to grow survive under extreme environmental conditions. In the present study, an ex-situ plant trapping method has been applied collect culturable microbial diversity soil from remote areas. Oryza sativa cv. Baldo Triticum durum Primadur were used as recruiters, while surrounding roots of glaberrima regions Mali (West Africa) was substrate for their growth. The endophytic recruited by two species belonged Proteobacteria Firmicutes, dominant genera Bacillus, Kosakonia, Enterobacter. These endophytes characterized analyzing some most common growth promoting traits. Halotolerant, inorganic phosphate-solubilizing N-fixing strains found, simultaneously showing these three We verified that ‘Baldo’ mostly halotolerant P-solubilizers endophytes, selected ‘Primadur’ mainly N-fixers. allowed isolate potential beneficial traits could be improvement rice wheat adverse

Язык: Английский

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Cultivar-specific wheat-associated microbiomes and metabolites in response to nitrogen deficiency

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 29, 2024

Abstract Background and Aims Nitrogen (N) deficiency in soil constrains plant growth, which may potentially be alleviated by beneficial microbes. However, there is limited knowledge of the plant-microbe interactions wheat cultivars with different N-use efficiency (NUE) under N deficiency. Methods We investigated responses root endosphere microorganisms as well metabolites two (cv. Mace Gladius) reported high low NUE, respectively, using a glasshouse experiment hydroponic three levels. Results The rhizosphere bacterial community shifted deficiency, but not its endosphere. Conversely, Gladius remained unchanged metagenomic analysis illustrated that growth motility Mace, Gladius, were enhanced A 4-fold increase octadecanoic acid extract suggesting potential role shaping rhizobacterial higher NUE. Conclusion Our study provides new evidence highlighting divergent wheat-associated to cultivars. In addition this, our findings suggest NUE selectively recruit communities through secreting specific metabolites, thereby enhancing their N-limited conditions.

Язык: Английский

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Microbial dynamics in rapidly transforming Arctic proglacial landscapes

PLOS Climate, Год журнала: 2024, Номер 3(6), С. e0000337 - e0000337

Опубликована: Июнь 25, 2024

Arctic glacial environments are rapidly changing, as the warms at a rate three to four times faster than global average (the phenomenon known amplification). Microorganisms uniquely adapted extreme and studying how ecological climatic feedback loops affect diversity of these communities is crucial characterisation vulnerable habitats. Glacial landscapes span wide range habitats, from glacier ice marine waters, encompass terrestrial, aquatic, interzonal systems. While shrinkage has been focus scientific attention, auxiliary habitats also impacted by rapid retreat. Auxiliary include terrestrial systems, such outwash plains, vegetated periglacial environments, aquatic glacier-fed streams, lakes, glacier-adjacent environments. Glacier recession drives high-impact changes in glacier-associated habitats: rising temperatures, increased light penetration nitrogen-to-phosphate ratios, increases availability glacier-derived organic compounds. In turn, microbial systems may experience nutrient dynamics shifts community structures. The exposure new lands retreating glaciers result dust dispersal into atmosphere. Here, we discuss effects climate change on microbiomes between large-scale processes Arctic. We characterise aspects ecosystems highlight importance preserving unseen biodiversity. then outline current capacities for conservation, focusing cryopreservation biobanking. Lastly, suggest future research directions steps that academic governmental institutions take foster collaboration with Indigenous communities.

Язык: Английский

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Cultivar-specific wheat-associated bacterial communities and metabolites in response to nitrogen deficiency

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 9, 2024

Background and Aims Nitrogen (N) deficiency in soil constrains plant growth, which may potentially be alleviated by beneficial bacterial communities. However, there is limited knowledge of the plant-bacteria interactions wheat cultivars with different N-use efficiency (NUE) under N deficiency. Methods We investigated responses root endosphere communities as well metabolites two (cv. Mace Gladius) reported high low NUE, respectively, using a glasshouse experiment hydroponic three levels. Results The rhizosphere community shifted deficiency, but not its endosphere. Conversely, Gladius remained unchanged metagenomic analysis illustrated increased detection genes related to growth motility Mace, Gladius, A 4-fold increase octadecanoic acid suggesting potential role shaping rhizobacterial higher NUE. Conclusion Our study highlights divergent wheat-associated microorganisms cultivars. found that NUE secretion, shaped communities, thereby enhancing their N-limited conditions.

Язык: Английский

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Cultivar-specific wheat-associated bacterial communities and metabolites in response to nitrogen deficiency

Plant and Soil, Год журнала: 2024, Номер unknown

Опубликована: Окт. 31, 2024

Язык: Английский

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Genome Insights into Beneficial Microbial Strains Composing SIMBA Microbial Consortia Applied as Biofertilizers for Maize, Wheat and Tomato

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2562 - 2562

Опубликована: Дек. 12, 2024

For the safe use of microbiome-based solutions in agriculture, genome sequencing strains composing inoculum is mandatory to avoid spread virulence and multidrug resistance genes carried by them through horizontal gene transfer other bacteria environment. Moreover, annotated genomes can enable design specific primers trace into soil provide insights molecular genetic mechanisms plant growth promotion biocontrol activity. In present work, sequences some members beneficial microbial consortia that have previously been tested greenhouse field trials as promising biofertilizers for maize, tomato wheat crops determined. Strains belong well-known plant-growth-promoting bacterial genera such Bacillus, Burkholderia, Pseudomonas Rahnella. The size ranged from 4.5 7.5 Mbp, carrying many spanning 4402 6697, a GC content 0.04% 3.3%. annotation revealed presence are implicated functions related antagonism, pathogenesis secondary metabolites possibly involved clusters protection against oxidative damage, confirming (PGP) activity selected strains. All target were found possess at least 3000 different PGP traits, belonging categories nitrogen acquisition, colonization plant-derived substrate usage, quorum sensing response biofilm formation and, lesser extent, fitness root colonization. No putatively identified. Overall, our study suggests application “plant probiotics” sustainable agriculture.

Язык: Английский

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