Extracellular Vesicles and Circulating Nucleic Acids,
Год журнала:
2022,
Номер
3(1), С. 63 - 86
Опубликована: Янв. 1, 2022
Extracellular
vesicles
(EVs),
which
are
nanocarriers
with
phospholipid
bilayer
structures
released
by
most
cells,
play
a
key
role
in
regulating
physiological
and
pathological
processes.
EVs
have
been
investigated
due
to
their
loading
capacity,
low
toxicity,
immunogenicity,
biofunctions.
Although
shown
good
potential
as
therapeutic
vehicles,
natural
poor
targeting
ability,
substantially
reduces
the
effect.
Through
addition
of
unit
into
membrane
surface
or
inside
engineering
technology,
agent
can
accumulate
specific
cells
tissues.
Here,
we
focus
on
mammalian
(MEVs)
bacterial
(BEVs),
two
common
types
biomedical
field.
In
this
review,
describe
engineered
MEVs
BEVs
promising
for
targeted
therapy
summarize
biogenesis,
isolation,
characterization
BEVs.
We
then
techniques
enhancement
ability
EVs.
Moreover,
applications
therapy,
including
treatment
cancer
brain
bone
disease.
believe
that
review
will
help
improve
understanding
BEVs,
thereby
promoting
application
clinical
translation.
Abstract
The
treatment
of
diabetic
wounds
remains
a
major
challenge
in
clinical
practice,
with
chronic
characterized
by
multiple
drug‐resistant
bacterial
infections,
angiopathy,
and
oxidative
damage
to
the
microenvironment.
Herein,
novel
situ
injectable
HA@MnO
2
/FGF‐2/Exos
hydrogel
is
introduced
for
improving
wound
healing.
Through
simple
local
injection,
this
able
form
protective
barrier
covering
wound,
providing
rapid
hemostasis
long‐term
antibacterial
protection.
MnO
/ε‐PL
nanosheet
catalyze
excess
H
O
produced
converting
it
,
thus
not
only
eliminating
harmful
effects
but
also
Moreover,
release
M2‐derived
Exosomes
(M2
Exos)
FGF‐2
growth
factor
stimulates
angiogenesis
epithelization,
respectively.
These
vivo
vitro
results
demonstrate
accelerated
healing
use
hydrogel,
presenting
viable
strategy
repair.
Advanced Materials,
Год журнала:
2023,
Номер
35(19)
Опубликована: Фев. 22, 2023
Diabetic
wound
(DW)
therapy
is
currently
a
big
challenge
in
medicine
and
strategies
to
enhance
neurogenesis
angiogenesis
have
appeared
be
promising
direction.
However,
the
current
treatments
failed
coordinate
simultaneously,
leading
an
increased
disability
rate
caused
by
DWs.
Herein,
whole-course-repair
system
introduced
hydrogel
concurrently
achieve
mutually
supportive
cycle
of
neurogenesis-angiogenesis
under
favorable
immune-microenvironment.
This
can
first
one-step
packaged
syringe
for
later
situ
local
injections
cover
wounds
long-termly
accelerated
healing
via
synergistic
effect
magnesium
ions
(Mg2+
)
engineered
small
extracellular
vesicles
(sEVs).
The
self-healing
bio-adhesive
properties
make
it
ideal
physical
barrier
At
inflammation
stage,
formulation
recruit
bone
marrow-derived
mesenchymal
stem
cells
sites
stimulate
them
toward
neurogenic
differentiation,
while
providing
immune
microenvironment
macrophage
reprogramming.
proliferation
stage
repair,
robust
occurs
newly
differentiated
neural
released
Mg2+
,
allowing
regenerative
take
place
at
site.
provides
novel
platform
combined
DW
therapy.
Journal of Nanobiotechnology,
Год журнала:
2021,
Номер
19(1)
Опубликована: Май 21, 2021
Abstract
Background
Enhanced
angiogenesis
can
promote
diabetic
wound
healing.
Mesenchymal
stem
cells
(MSCs)-derived
exosomes,
which
are
cell-free
therapeutics,
promising
candidates
for
the
treatment
of
The
present
study
aimed
to
investigate
effect
exosomes
derived
from
MSCs
pretreated
with
pioglitazone
(PGZ-Exos)
on
Results
We
isolated
PGZ-Exos
supernatants
pioglitazone-treated
BMSCs
and
found
that
significantly
cell
viability
proliferation
Human
Umbilical
Vein
Vascular
Endothelial
Cells
(HUVECs)
injured
by
high
glucose
(HG).
enhanced
biological
functions
HUVECs,
including
migration,
tube
formation,
repair
VEGF
expression
in
vitro.
In
addition,
promoted
protein
p-AKT,
p-PI3K
p-eNOS
suppressed
PTEN.
LY294002
inhibited
function
HUVECs
through
inhibition
PI3K/AKT/eNOS
pathway.
vivo
modeling
rat
wounds
showed
pretreatment
therapeutic
efficacy
MSCs-derived
accelerated
healing
via
angiogenesis.
collagen
deposition,
ECM
remodeling
CD31
expression,
indicating
adequate
Conclusions
promoting
angiogenic
activation
This
offers
a
novel
therapy
treating
Graphic
abstract
Military Medical Research,
Год журнала:
2022,
Номер
9(1)
Опубликована: Ноя. 19, 2022
Abstract
Bone,
cartilage,
and
soft
tissue
regeneration
is
a
complex
spatiotemporal
process
recruiting
variety
of
cell
types,
whose
activity
interplay
must
be
precisely
mediated
for
effective
healing
post-injury.
Although
extensive
strides
have
been
made
in
the
understanding
immune
microenvironment
processes
governing
bone,
regeneration,
clinical
translation
these
mechanisms
remains
challenge.
Regulation
increasingly
becoming
favorable
target
regeneration;
therefore,
an
in-depth
communication
between
cells
functional
would
valuable.
Herein,
we
review
regulatory
role
promotion
maintenance
stem
states
context
repair
regeneration.
We
discuss
roles
various
subsets
introduce
novel
strategies,
example,
biomaterial-targeting
activity,
aimed
at
regulating
healing.
Understanding
crosstalk
pathways
may
shed
light
on
new
therapeutic
opportunities
enhancing
through
regulation
microenvironment.
Drug Delivery,
Год журнала:
2020,
Номер
27(1), С. 745 - 755
Опубликована: Янв. 1, 2020
It
is
reported
that
quercetin
(Que)
can
prevent
tau
pathology
and
induce
neuroprotection
by
improving
cognitive
functional
symptoms
in
the
treatment
of
Alzheimer's
disease
(AD).
However,
its
clinical
application
has
been
limited
due
to
poor
brain
targeting
bioavailability.
Exosomes
are
considered
as
cargo
carriers
for
intercellular
communication
especially
serve
a
natural
important
drug
delivery
platform
achieving
better
central
neurological
diseases.
Here,
we
developed
plasma
exosomes
(Exo)
loaded
with
Que
(Exo-Que)
improve
bioavailability,
enhance
potently
ameliorate
dysfunction
okadaic
acid
(OA)-induced
AD
mice.
Our
results
showed
Exo-Que
improved
well
significantly
enhanced
bioavailability
Que.
Furthermore,
compared
free
Que,
relieved
inhibiting
cyclin-dependent
kinase
5
(CDK5)-mediated
phosphorylation
Tau
reducing
formation
insoluble
neurofibrillary
tangles
(NFTs),
suggesting
therapeutic
potential
AD.
Chemical Engineering Journal,
Год журнала:
2021,
Номер
426, С. 130634 - 130634
Опубликована: Июнь 6, 2021
Diabetic
wounds
represent
a
pressing
concern
affecting
the
health
and
quality
of
life
patients.
Despite
continuous
improvement
in
therapeutic
strategy,
diabetic
remain
worldwide
challenge.
Vascular
dysfunction,
part
due
to
hyperglycemia,
is
well-identified
factor
contributing
inadequate
healing
wounds.
In
current
study,
we
utilize
an
extrusion-based
cryogenic
3D
printing
technology
construct
decellularized
small
intestinal
submucosa
(SIS)
combined
with
mesoporous
bioactive
glass
(MBG)
exosomes
fabricate
produce
scaffold
dressing
(SIS/[email
protected])
which
permits
sustained
release
exosomes.
The
SIS/[email
protected]
hydrogel
scaffolds
possess
good
structure
suitable
porosity,
biocompatibility
hemostasis
ability,
could
promote
proliferation,
migration
angiogenesis
Human
umbilical
vein
endothelial
cells
(HUVECs).
results
vivo
indicate
that
accelerate
wound
through
increasing
blood
flow
stimulating
process
wound.
also
granulation
tissue
formation,
well-organized
collagen
fiber
deposition,
functional
new
vessel
growth,
factors
promoting
healing.
Taken
together,
this
research
presents
promising
novel
strategy
for
treatment
Military Medical Research,
Год журнала:
2023,
Номер
10(1)
Опубликована: Авг. 17, 2023
Abstract
Skin
wounds
are
characterized
by
injury
to
the
skin
due
trauma,
tearing,
cuts,
or
contusions.
As
such
injuries
common
all
human
groups,
they
may
at
times
represent
a
serious
socioeconomic
burden.
Currently,
increasing
numbers
of
studies
have
focused
on
role
mesenchymal
stem
cell
(MSC)-derived
extracellular
vesicles
(EVs)
in
wound
repair.
cell-free
therapy,
MSC-derived
EVs
shown
significant
application
potential
field
repair
as
more
stable
and
safer
option
than
conventional
therapy.
Treatment
based
can
significantly
promote
damaged
substructures,
including
regeneration
vessels,
nerves,
hair
follicles.
In
addition,
inhibit
scar
formation
affecting
angiogenesis-related
antifibrotic
pathways
promoting
macrophage
polarization,
angiogenesis,
proliferation,
migration,
inhibiting
excessive
matrix
production.
Additionally,
these
structures
serve
scaffold
for
components
used
repair,
be
developed
into
bioengineered
support
trauma
Through
formulation
standardized
culture,
isolation,
purification,
drug
delivery
strategies,
exploration
detailed
mechanism
will
allow
them
clinical
treatments
conclusion,
EVs-based
therapies
important
prospects
Here
we
provide
comprehensive
overview
their
current
status,
potential,
associated
drawbacks.
