Engineered mammalian and bacterial extracellular vesicles as promising nanocarriers for targeted therapy DOI Open Access
Han Liu, Zhen Geng, Jiacan Su

и другие.

Extracellular Vesicles and Circulating Nucleic Acids, Год журнала: 2022, Номер 3(1), С. 63 - 86

Опубликована: Янв. 1, 2022

Extracellular vesicles (EVs), which are nanocarriers with phospholipid bilayer structures released by most cells, play a key role in regulating physiological and pathological processes. EVs have been investigated due to their loading capacity, low toxicity, immunogenicity, biofunctions. Although shown good potential as therapeutic vehicles, natural poor targeting ability, substantially reduces the effect. Through addition of unit into membrane surface or inside engineering technology, agent can accumulate specific cells tissues. Here, we focus on mammalian (MEVs) bacterial (BEVs), two common types biomedical field. In this review, describe engineered MEVs BEVs promising for targeted therapy summarize biogenesis, isolation, characterization BEVs. We then techniques enhancement ability EVs. Moreover, applications therapy, including treatment cancer brain bone disease. believe that review will help improve understanding BEVs, thereby promoting application clinical translation.

Язык: Английский

All‐in‐One: Multifunctional Hydrogel Accelerates Oxidative Diabetic Wound Healing through Timed‐Release of Exosome and Fibroblast Growth Factor DOI Creative Commons
Yuan Xiong, Lang Chen, Pei Liu

и другие.

Small, Год журнала: 2021, Номер 18(1)

Опубликована: Ноя. 17, 2021

Abstract The treatment of diabetic wounds remains a major challenge in clinical practice, with chronic characterized by multiple drug‐resistant bacterial infections, angiopathy, and oxidative damage to the microenvironment. Herein, novel situ injectable HA@MnO 2 /FGF‐2/Exos hydrogel is introduced for improving wound healing. Through simple local injection, this able form protective barrier covering wound, providing rapid hemostasis long‐term antibacterial protection. MnO /ε‐PL nanosheet catalyze excess H O produced converting it , thus not only eliminating harmful effects but also Moreover, release M2‐derived Exosomes (M2 Exos) FGF‐2 growth factor stimulates angiogenesis epithelization, respectively. These vivo vitro results demonstrate accelerated healing use hydrogel, presenting viable strategy repair.

Язык: Английский

Процитировано

241

A Whole‐Course‐Repair System Based on Neurogenesis‐Angiogenesis Crosstalk and Macrophage Reprogramming Promotes Diabetic Wound Healing DOI Creative Commons
Yuan Xiong, Ze Lin,

Pengzhen Bu

и другие.

Advanced Materials, Год журнала: 2023, Номер 35(19)

Опубликована: Фев. 22, 2023

Diabetic wound (DW) therapy is currently a big challenge in medicine and strategies to enhance neurogenesis angiogenesis have appeared be promising direction. However, the current treatments failed coordinate simultaneously, leading an increased disability rate caused by DWs. Herein, whole-course-repair system introduced hydrogel concurrently achieve mutually supportive cycle of neurogenesis-angiogenesis under favorable immune-microenvironment. This can first one-step packaged syringe for later situ local injections cover wounds long-termly accelerated healing via synergistic effect magnesium ions (Mg2+ ) engineered small extracellular vesicles (sEVs). The self-healing bio-adhesive properties make it ideal physical barrier At inflammation stage, formulation recruit bone marrow-derived mesenchymal stem cells sites stimulate them toward neurogenic differentiation, while providing immune microenvironment macrophage reprogramming. proliferation stage repair, robust occurs newly differentiated neural released Mg2+ , allowing regenerative take place at site. provides novel platform combined DW therapy.

Язык: Английский

Процитировано

221

Exosomes derived from pioglitazone-pretreated MSCs accelerate diabetic wound healing through enhancing angiogenesis DOI Creative Commons
Yiqiang Hu, Ranyang Tao, Lang Chen

и другие.

Journal of Nanobiotechnology, Год журнала: 2021, Номер 19(1)

Опубликована: Май 21, 2021

Abstract Background Enhanced angiogenesis can promote diabetic wound healing. Mesenchymal stem cells (MSCs)-derived exosomes, which are cell-free therapeutics, promising candidates for the treatment of The present study aimed to investigate effect exosomes derived from MSCs pretreated with pioglitazone (PGZ-Exos) on Results We isolated PGZ-Exos supernatants pioglitazone-treated BMSCs and found that significantly cell viability proliferation Human Umbilical Vein Vascular Endothelial Cells (HUVECs) injured by high glucose (HG). enhanced biological functions HUVECs, including migration, tube formation, repair VEGF expression in vitro. In addition, promoted protein p-AKT, p-PI3K p-eNOS suppressed PTEN. LY294002 inhibited function HUVECs through inhibition PI3K/AKT/eNOS pathway. vivo modeling rat wounds showed pretreatment therapeutic efficacy MSCs-derived accelerated healing via angiogenesis. collagen deposition, ECM remodeling CD31 expression, indicating adequate Conclusions promoting angiogenic activation This offers a novel therapy treating Graphic abstract

Язык: Английский

Процитировано

213

The role of the immune microenvironment in bone, cartilage, and soft tissue regeneration: from mechanism to therapeutic opportunity DOI Creative Commons
Yuan Xiong, Bobin Mi, Ze Lin

и другие.

Military Medical Research, Год журнала: 2022, Номер 9(1)

Опубликована: Ноя. 19, 2022

Abstract Bone, cartilage, and soft tissue regeneration is a complex spatiotemporal process recruiting variety of cell types, whose activity interplay must be precisely mediated for effective healing post-injury. Although extensive strides have been made in the understanding immune microenvironment processes governing bone, regeneration, clinical translation these mechanisms remains challenge. Regulation increasingly becoming favorable target regeneration; therefore, an in-depth communication between cells functional would valuable. Herein, we review regulatory role promotion maintenance stem states context repair regeneration. We discuss roles various subsets introduce novel strategies, example, biomaterial-targeting activity, aimed at regulating healing. Understanding crosstalk pathways may shed light on new therapeutic opportunities enhancing through regulation microenvironment.

Язык: Английский

Процитировано

188

Brain delivery of quercetin-loaded exosomes improved cognitive function in AD mice by inhibiting phosphorylated tau-mediated neurofibrillary tangles DOI Creative Commons

Yao Qi,

Lin Guo, Yibin Jiang

и другие.

Drug Delivery, Год журнала: 2020, Номер 27(1), С. 745 - 755

Опубликована: Янв. 1, 2020

It is reported that quercetin (Que) can prevent tau pathology and induce neuroprotection by improving cognitive functional symptoms in the treatment of Alzheimer's disease (AD). However, its clinical application has been limited due to poor brain targeting bioavailability. Exosomes are considered as cargo carriers for intercellular communication especially serve a natural important drug delivery platform achieving better central neurological diseases. Here, we developed plasma exosomes (Exo) loaded with Que (Exo-Que) improve bioavailability, enhance potently ameliorate dysfunction okadaic acid (OA)-induced AD mice. Our results showed Exo-Que improved well significantly enhanced bioavailability Que. Furthermore, compared free Que, relieved inhibiting cyclin-dependent kinase 5 (CDK5)-mediated phosphorylation Tau reducing formation insoluble neurofibrillary tangles (NFTs), suggesting therapeutic potential AD.

Язык: Английский

Процитировано

172

Emerging roles of exosomal miRNAs in diabetes mellitus DOI
Xiaoyun He, Gaoyan Kuang, Yongrong Wu

и другие.

Clinical and Translational Medicine, Год журнала: 2021, Номер 11(6)

Опубликована: Июнь 1, 2021

Язык: Английский

Процитировано

168

Hierarchical micro/nanofibrous scaffolds incorporated with curcumin and zinc ion eutectic metal organic frameworks for enhanced diabetic wound healing via anti-oxidant and anti-inflammatory activities DOI
Yitong Wang, Ting Ying,

Jinxiu Li

и другие.

Chemical Engineering Journal, Год журнала: 2020, Номер 402, С. 126273 - 126273

Опубликована: Июль 16, 2020

Язык: Английский

Процитировано

144

Cryogenic 3D printed hydrogel scaffolds loading exosomes accelerate diabetic wound healing DOI Creative Commons
Yiqiang Hu, Bin Wu, Yuan Xiong

и другие.

Chemical Engineering Journal, Год журнала: 2021, Номер 426, С. 130634 - 130634

Опубликована: Июнь 6, 2021

Diabetic wounds represent a pressing concern affecting the health and quality of life patients. Despite continuous improvement in therapeutic strategy, diabetic remain worldwide challenge. Vascular dysfunction, part due to hyperglycemia, is well-identified factor contributing inadequate healing wounds. In current study, we utilize an extrusion-based cryogenic 3D printing technology construct decellularized small intestinal submucosa (SIS) combined with mesoporous bioactive glass (MBG) exosomes fabricate produce scaffold dressing (SIS/[email protected]) which permits sustained release exosomes. The SIS/[email protected] hydrogel scaffolds possess good structure suitable porosity, biocompatibility hemostasis ability, could promote proliferation, migration angiogenesis Human umbilical vein endothelial cells (HUVECs). results vivo indicate that accelerate wound through increasing blood flow stimulating process wound. also granulation tissue formation, well-organized collagen fiber deposition, functional new vessel growth, factors promoting healing. Taken together, this research presents promising novel strategy for treatment

Язык: Английский

Процитировано

111

Mesenchymal stem cell-derived extracellular vesicles in skin wound healing: roles, opportunities and challenges DOI Creative Commons

Jiayi Ding,

Minjiang Chen, Lingfeng Wu

и другие.

Military Medical Research, Год журнала: 2023, Номер 10(1)

Опубликована: Авг. 17, 2023

Abstract Skin wounds are characterized by injury to the skin due trauma, tearing, cuts, or contusions. As such injuries common all human groups, they may at times represent a serious socioeconomic burden. Currently, increasing numbers of studies have focused on role mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in wound repair. cell-free therapy, MSC-derived EVs shown significant application potential field repair as more stable and safer option than conventional therapy. Treatment based can significantly promote damaged substructures, including regeneration vessels, nerves, hair follicles. In addition, inhibit scar formation affecting angiogenesis-related antifibrotic pathways promoting macrophage polarization, angiogenesis, proliferation, migration, inhibiting excessive matrix production. Additionally, these structures serve scaffold for components used repair, be developed into bioengineered support trauma Through formulation standardized culture, isolation, purification, drug delivery strategies, exploration detailed mechanism will allow them clinical treatments conclusion, EVs-based therapies important prospects Here we provide comprehensive overview their current status, potential, associated drawbacks.

Язык: Английский

Процитировано

101

Exosomal ncRNAs: Novel therapeutic target and biomarker for diabetic complications DOI

Yuxin Xu,

Shengdan Pu, Xin Li

и другие.

Pharmacological Research, Год журнала: 2022, Номер 178, С. 106135 - 106135

Опубликована: Фев. 19, 2022

Язык: Английский

Процитировано

97