Quantitative Imaging of MicroRNA‐21 In Vivo for Real‐Time Monitoring of the Cancer Initiation and Progression

Advanced Functional Materials, Год журнала: 2024, Номер 34(45)

Опубликована: Авг. 28, 2024

Abstract MicroRNA‐21 (MiR‐21) has been confirmed to be upregulated in tumors, and its abnormal expression is closely associated with tumor occurrence. However, the traditional imaging methods are limited qualitative of miR‐21, no effective strategy developed for monitoring concentration vivo during cancer initiation progression. Herein, a biosensor created utilizing NIR‐II ratiometric fluorescent nanoprobe quantitatively monitor dynamic alterations miR‐21 levels vivo. The (termed DCNP@DNA2@IR806) constructed by introducing IR806 as donor down‐conversion nanoparticles (DCNP) acceptor, using DNA linkers. Upon miR‐21‐responsive nanoprobe, 1550 nm signal DCNP stimulated 808 laser (F 1550, 808Ex ) increased because close proximity subsequent non‐radiative energy transfer (NRET). Meanwhile, 980 980Ex remained stable absence NRET. This reliable indicator holds promise further enhancing understanding microRNAs‐based molecular mechanisms underlying progression, laying foundation early diagnosis microRNAs‐related diseases.

Язык: Английский

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Integrating DNA logic computation and Self-Replication on nanospheres for amplified detection of Dual miRNAs

Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 157160 - 157160

Опубликована: Окт. 1, 2024

Язык: Английский

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Redox-Stimulated Catalytic DNA Circuit for High-fidelity Imaging of MicroRNA and in situ Interpretation of the Relevant Regulatory Pathway

Biosensors and Bioelectronics, Год журнала: 2024, Номер 272, С. 117109 - 117109

Опубликована: Дек. 28, 2024

Язык: Английский

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Autocatalytic DNA circuitries

Chemical Society Reviews, Год журнала: 2024, Номер 53(22), С. 10878 - 10899

Опубликована: Янв. 1, 2024

Autocatalysis, a self-sustained replication process where at least one of the products functions as catalyst, plays pivotal role in life's evolution, from genome duplication to emergence autocatalytic subnetworks cell division and metabolism. Leveraging their programmability, controllability, rich functionalities, DNA molecules have become cornerstone for engineering circuits, driving diverse technological applications. In this tutorial review, we offer comprehensive survey recent advances circuits practical implementations. We delve into fundamental principles underlying construction these highlighting reliance on DNAzyme biocatalysis, enzymatic catalysis, dynamic hybridization assembly. The discussed circuitry techniques revolutionized ultrasensitive sensing biologically significant molecules, encompassing genomic DNAs, RNAs, viruses, proteins. Furthermore, amplicons produced by serve building blocks higher-order nanostructures, facilitating biomimetic behaviors such high-performance intracellular bioimaging precise algorithmic summarize applications extensively address current challenges, potential solutions, future trajectories circuits. This review promises novel insights advancement utilization across bioanalysis, biomedicine, biomimetics.

Язык: Английский

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Principles of nucleic acid toehold mediated strand displacement (TMSD) reaction model and its applications in cell environment

Biomaterials Science, Год журнала: 2023, Номер 11(15), С. 5060 - 5077

Опубликована: Янв. 1, 2023

We summarize the principles of nucleic acid TMSD reaction and highlight successful development application in cell environment.

Язык: Английский

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Integration of Isothermal Enzyme‐Free Nucleic Acid Circuits for High‐Performance Biosensing Applications

ChemPlusChem, Год журнала: 2023, Номер 88(10)

Опубликована: Сен. 14, 2023

The isothermal enzyme-free nucleic acid amplification method plays an indispensable role in biosensing by virtue of its simple, robust, and highly efficient properties without the assistance temperature cycling or/and enzymatic biocatalysis. Up to now, has been extensively utilized for biological assays achieved sensitive detection various targets, including DNAs, RNAs, small molecules, proteins, even cells. In this Review, mechanisms entropy-driven reaction, hybridization chain catalytic hairpin assembly DNAzyme are concisely described their recent application as biosensors is comprehensively summarized. Furthermore, current problems developments these DNA circuits also discussed.

Язык: Английский

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Intelligent Sense‐on‐Demand DNA Circuits for Amplified Bioimaging in Living Cells

Опубликована: Май 3, 2024

As a typical toolkit of biomolecular engineering, DNA nanotechnology aims to fabricate delicate molecular machines with unique functions and assemble DNA-based circuits capable performing diverse tasks. Compared enzymatic circuits, these enzyme-free strategies are more suitable for implementing bioimaging in situ because they do not require the delivery exotic enzymes their function. Despite conspicuous progress made construction DNAzyme-based autocatalytic machines, there still urgent challenges that need be addressed before can extensively utilized amplified imaging biomolecules. pre-designed circuits. The stimuli-responsive strategy has dramatically facilitated development intelligent sense-on-demand Nonenzymatic rely on programmable nucleic-acid-alone elements amplify an initiator-induced signal.

Язык: Английский

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Enzyme-Accelerated Catalytic DNA Circuits Enable Rapid and One-pot Detection of Bacterial Pathogens

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 10, 2024

Catalytic DNA circuits, serving as signal amplification strategies, can enable simple and accurate detection of pathogenic bacteria in complex matrices but suffer from low reaction rates depths. Herein, we design an enzyme-accelerated catalytic hairpin assembly (EACHA) which duplex products are converted into reactants to continue participating the next circuit with assistance RNase H. Profiting high recyclability reactants, EACHA exhibits approximately 37.6-fold enhancement rate constant a two-order-of-magnitude improvement sensitivity compared conventional (CHA). By integrating allosteric probe EACHA, one-pot method is developed for rapid direct S. enterica Enteritidis. This capable detecting 15 CFU mL^{− 1} S. Enteritidis within 20 min, superior that real-time PCR. testing 60 milk samples, demonstrate this method's accuracy discriminating contaminated area under curve (AUC) 0.997. Moreover, be employed accurately diagnose early-stage infected mice, AUC 1.00 feces samples 0.986 serum samples. Therefore, study offers feasible identifying pathogens matrices.

Язык: Английский

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Titanium Carbide-Based Spatiotemporally Selectable-Activated Entropy-Driven DNA Nanoplatform for Amplified MicroRNA Imaging and Photothermal Therapy In Vivo

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Сен. 29, 2024

Engineering an elaborate nanotheranostic platform that can achieve spatiotemporally selective microRNA (miRNA) imaging and imaging-guided therapy in time is critical for precise cancer diagnosis efficient treatment, yet remains a challenge. Herein, we present on-site-activatable (Ti

Язык: Английский

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