Fluorescein Derivative Monomer for the Production of Fluorescent Latex Particles

Particle & Particle Systems Characterization, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Abstract The use of fluorescent latex particles (FLP) as a tool for microscopy imaging or flux cytometry is an excellent choice diagnosis, research, and development. They are suitable complex formation with several biological molecules macromolecules. A simple cost‐effective method here proposed to produce FLP. To this effect, the synthesis monomer, capable being copolymerized vinyl monomers, achieved by reaction between fluorescein glycidyl methacrylate, monitored UV–vis, IR, NMR, Fluorescence spectrometry. carried out emulsion copolymerization styrene, methacrylic acid, monomer. Latexes characterized measuring styrene/methacrylic acid conversion, average particle diameter, fluorescence, surface charge, group density. Conversion reaches ≈90%, negatively charged 130–260 nm obtained, which proved have high fluorescence emission maximum 511 nm. Cell staining using obtained particles. Observation FLP‐treated cells confocal exhibited intense within all internal media, fluorescein‐like pattern. production stable particles, controlled size incorporation, interacting proteins efficiently, confirmed.

Язык: Английский

---

Site‐Specific Polymer‐Protein‐Polymer Conjugates for the Preparation of Dual Responsive Multilayer Nanoparticles

Small, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Abstract Protein‐polymer‐based materials demonstrate high potential in advanced applications. However, controlled combinations of multiple proteins and polymers to obtain multimaterial systems is limited due the complexity retaining protein structure function achieving structural control for simultaneously. Here, first combination a rebridging agent thiol‐induced, light‐activated radical polymerization (TIRP) introduced directly enable site‐specific conjugation two different native proteins. Specifically, poly( N ‐isopropyacrylamide) (pNIPAM) attached bovine serum albumin (BSA), followed by incorporation new agent, initiating second TIRP introduce glycopolymer, giving highly defined pNIPAM‐BSA‐glycopolymer conjugates. Above lower critical solution temperature (LCST), nanoparticles with glycopolymer corona are formed. The addition glycan‐specific lectin leads formation so‐called multilayer nanoparticles. Depending on sequence stimuli, particles can either undergo step‐wise or one‐step disassembly. Furthermore, controlling ratio binding/non‐binding glycopolymers nanoparticles, distinct large clusters be Thus, dual‐responsive multilayered polymer‐protein now accessible programmable material properties such as assembly disassembly while maintaining protein's thus function.

Язык: Английский

---

Redox-Initiated RAFT Emulsion Polymerization-Induced Self-Assembly of β-Ketoester Functional Monomers

Polymers, Год журнала: 2025, Номер 17(7), С. 870 - 870

Опубликована: Март 24, 2025

Amphiphilic block copolymers are essential for developing advanced polymer nanomaterials with applications in bioimaging, drug delivery, and nanoreactors. In this study, we successfully synthesized functional copolymer assemblies at high concentrations through redox-initiated reversible addition-fragmentation chain transfer (RAFT) emulsion polymerization of 2-(acetoacetoxy)ethyl methacrylate (AEMA), a β-ketoester monomer. Utilizing redox initiation system 50 °C, produced poly(poly(ethylene glycol) methyl ether methacrylate)-b-PAEMA (PPEGMAn-PAEMAm). Kinetic studies demonstrated rapid monomer conversion exceeding 95% within 30 min, distinct phases driven by micelle formation depletion. Transmission Electron Microscopy (TEM) Dynamic Light Scattering (DLS) revealed the diverse morphologies, including worm-like, vesicular structures, spherical micelles, depending on macro-CTA molecular weight concentration. Additionally, post-polymerization modification aggregation-induced emission (AIE) luminogens, such as 1-(4-aminophenyl)-1,2,2-tristyrene (TPE-NH2), resulted AIE-active exhibiting strong fluorescence aqueous dispersions. These also exhibited good biocompatibility. findings demonstrate efficacy RAFT fabricating functional, scalable potential field life sciences.

Язык: Английский

---

J-Aggregated Fluorescence Nanoparticles with Multichromatic Performance Enable Sensitive LFIA Platform

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Апрель 18, 2025

The application of conventional lateral flow immunoassay (LFIA) remains challenging due to its single signal readout and poor sensitivity. In this work, we developed J-aggregated fluorescence nanoparticles (JNPs) with multichromatic performance employed them as reporters for the simultaneous sensitive detection T-2 AFB1 toxins on LFIA platform. Upon strong confinement effect nanoparticles, emission region JNPs was red-shifted from ∼545 ∼620 nm, exhibiting a J-aggregation phenomenon. Subsequently, two green red were development multiplexed platform (JNPs-LFIA) on-site quantitative toxins, limits 0.645 ng mL-1 0.0035 mL-1, which 3.5-fold 12.2-fold lower than those AuNPs-LFIA, respectively. Overall, work provides an efficient strategy design immunoprobes promotes in biosensing application.

Язык: Английский

---

Fluorescence-Selective Absorption and Circularly Polarized Fluorescence Energy Transfer Assist the Generation of Multicolor Circularly Polarized Luminescence in Chiral Helical Polyacetylene-Based Janus Nanofibers

ACS Macro Letters, Год журнала: 2024, Номер 13(6), С. 673 - 680

Опубликована: Май 16, 2024

Chiroptical nanomaterials with circularly polarized luminescence (CPL) performance have aroused increasing attention. Herein, multicolor CPL-active Janus nanofibers are prepared through a simple parallel electrospinning method using chiral helical polyacetylenes as the source and achiral fluorophores fluorescent source. Interestingly, despite direct spatial isolation between component component, blue green CPL emissions can still be obtained due to fluorescence-selective absorption behavior of polyacetylenes, satisfactory dissymmetric factor (glum) 2 × 10-2 2.5 10-3, respectively. Moreover, by taking advantage circular polarization fluorescence energy transfer process, red emission is further achieved donors fluorophore an acceptor. The present work offers facile approach prepare multilevel-structured chiroptical materials promising application potentials in flexible photoelectric device.

Язык: Английский

---

Highly efficient dye adsorption by hierarchical porous SA/PVA/ZIF-8 composite microgels prepared via microfluidics

Carbohydrate Polymers, Год журнала: 2024, Номер 350, С. 123016 - 123016

Опубликована: Ноя. 19, 2024

Язык: Английский

---

Preparation of fluorescent polyurethane microspheres and their applications as reusable sensor for 4-nitrophenol detection and as microplastics model for visualizing polyurethane in cells and zebrafish

Journal of Colloid and Interface Science, Год журнала: 2024, Номер 673, С. 550 - 563

Опубликована: Июнь 13, 2024

Язык: Английский

---

Dansylated Nitrile N‐Oxide as the Fluorescent Dye Clickable to Unsaturated Bonds without Catalyst

Chemistry - A European Journal, Год журнала: 2024, Номер 30(14)

Опубликована: Фев. 5, 2024

Fluorescent polymeric materials have been exploited in the fields of aesthetical purposes, biomedical engineering, and three-dimensional printing applications. While fluorescent are prepared by polymerization monomer or blending a dye with common polymer, covalent immobilization onto polymers is not practical technique. In this paper, dansylated nitrile N-oxide (Dansyl-NO) has designed synthesized to be stable as derivative 2-hydroxy-1-naphthaldehyde. Dansyl-NO shows good reactivity an alkene alkyne give Dansyl-Ene Dansyl-Yne, respectively, it hardly reacts nitrile. The results indicate that serves clickable alkenes alkynes. To know effects solvent on properties, UV-vis fluorescence spectra measured three solvents. solvatochromism, which appears red-shifted along increase polarity. Poly(styrene-co-butadiene) directly modified SB. emission spectrum SB blue-shifted compared Dansyl-Ene. blue-shift could possibly attributed presence less polar polymer skeleton around dansyl moieties

Язык: Английский

---

Synthesis of fluorescent polymers from hydrostyryl pyridinium derivatives and their multichromatic fluorescence behaviors

European Polymer Journal, Год журнала: 2024, Номер 208, С. 112850 - 112850

Опубликована: Фев. 13, 2024

Язык: Английский

---

Foundation of sensory polymers

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 1 - 58

Опубликована: Янв. 1, 2024

Язык: Английский

---