Mini crRNA-Mediated CRISPR/Cas12a System Enhanced Imaging of Multiple MicroRNAs in Cells DOI Creative Commons
Xiaolong Chen, Qianyi You, Yawen Guo

и другие.

Chemical & Biomedical Imaging, Год журнала: 2024, Номер unknown

Опубликована: Дек. 19, 2024

RNA imaging in live cells can provide comprehensive information on the expression, localization, degradation, storage, and regulation of cells, which is crucial for basic biology clinical research. Our previous research finds that slicing facilitated crRNA typical CRISPR/Cas12a system at a fitted site did not affect its trans-cleavage activity, was previously reported to be triggered by targeted ssDNA or dsDNA, mini crRNA-mediated (MCM-CRISPR/Cas12a) proposed. Here, we further apply it enhanced MicroRNAs designing activator as molecular beacon (MB), form hybrid double-stranded structure DNA/RNA with MicroRNA. When MicroRNA present, hairpin MB opened emits fluorescence. Simultaneously, DNA-RNA formed activates activity LbCas12a, partially cleaving single-stranded DNA extended from enhancing fluorescence intensity system. We designed MCM-CRISPR/Cas12a miRNA-21, miRNA-155, miRNA-10b successfully applied sensitive specific these both inside outside cells. This study provides new idea multiple within important studying distribution dynamic changes

Язык: Английский

Recent advances on DNAzyme for bioimaging, biosensing and cancer therapy DOI
Pei Sun,

Hongquan Gou,

Xinran Che

и другие.

Chemical Communications, Год журнала: 2024, Номер 60(78), С. 10805 - 10821

Опубликована: Янв. 1, 2024

DNAzymes, a class of single-stranded catalytic DNA with good stability, high activity, and easy synthesis, functionalization modification properties, have garnered significant interest in the realm biosensing bioimaging. Their integration fluorescent dyes or chemiluminescent moieties has led to remarkable bioimaging outcomes, while DNAzyme-based biosensors demonstrated robust sensitivity selectivity detecting metal ions, nucleic acids, proteins, enzyme activities, exosomes, bacteria microorganisms. In addition, by delivering DNAzymes into tumor cells, mRNA therein can be cleaved regulate expression corresponding which further propelled application cancer gene therapy synergistic therapy. This paper reviews strategies for screening attractive such as SELEX high-throughput sequencing, briefly describes amplification mainly include hairpin assembly (CHA), walker, hybridization chain reaction (HCR), origami, CRISPR-Cas12a, rolling circle (RCA), aptamers. applications bioimaging, biosensing, are also highlighted. Subsequently, possible challenges these practical pointed out, future research directions suggested.

Язык: Английский

Процитировано

6
Carbon dots with high brightness and stability towards efficient Cr(Ⅵ) and l-arginine sensors DOI

Xuanqi Hao,

Xiao Zhang, Jianrong Wang

и другие.

Microchemical Journal, Год журнала: 2024, Номер 207, С. 112092 - 112092

Опубликована: Ноя. 2, 2024

Язык: Английский

Процитировано

6
FRAME: flap endonuclease 1-engineered PAM module for precise and sensitive modulation of CRISPR/Cas12a trans-cleavage activity DOI Creative Commons

Tongshan Zuo,

Chen Shen, Zhen Xie

и другие.

Nucleic Acids Research, Год журнала: 2024, Номер 52(19), С. 11884 - 11894

Опубликована: Сен. 24, 2024

Abstract CRISPR/Cas12a system, renowned for its precise recognition and efficient nucleic acid cleavage capabilities, has demonstrated remarkable performance in molecular diagnostics biosensing. However, the reported Cas12a activity regulation methods often involved intricate CRISPR RNA (crRNA) structural adjustments or costly chemical modifications, which limited their applications. Here, we a unique enzyme engineering strategy using flap endonuclease 1 (FEN1) to regulate accessibility of protospacer adjacent motif (PAM) module double-stranded DNA activator (FRAME). By identifying three-base overlapping structure between target inputs substrate, FEN1 selectively cleaved released 5′-flap containing ‘TTTN’ sequence, triggered secondary while forming nicked PAM, ultimately achieving sensitive switching Cas12a’s activity. The FRAME exemplified ‘two birds with one stone’ principle, as it not only precisely programmed but also simultaneously isothermal cyclic amplification. Moreover, was applied construct sensing platform detecting myeloperoxidase miR-155, high sensitivity specificity. Importantly, proved versatility multiple targets single crRNA without redesign. Collectively, opens up novel avenue modulating activity, promising immense potential realm medical diagnostics.

Язык: Английский

Процитировано

5
DNA logic nanomachine for the accurate identification of multiple microRNAs in tumor cells DOI
Zhe Dong, Wenjun Zhu,

Yingjie Ren

и другие.

Talanta, Год журнала: 2025, Номер 287, С. 127590 - 127590

Опубликована: Янв. 13, 2025

Язык: Английский

Процитировано

0
Design of Amphiphilic Carbon Dots for Producing Stable CO2 Foams as Eco-Friendly Fluids for Enhanced Oil Recovery DOI
Qianqian Gao,

Peng Wei,

Bolin Lv

и другие.

ACS Sustainable Chemistry & Engineering, Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

Язык: Английский

Процитировано

0
Recent Advances in the Design and Application of Asymmetric Carbon‐Based Materials DOI Creative Commons

Chenqi Cui,

Haoxuan Ma,

Juan Du

и другие.

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 26, 2025

Abstract Asymmetric carbon‐based materials (ACBMs) have received significant attention in scientific research due to their unique structures and properties. Through the introduction of heterogeneous atoms construction asymmetric ordered/disordered structures, ACBMs are optimized terms electrical conductivity, pore structure, chemical composition exhibit multiple properties such as hydrophilicity, hydrophobicity, optical characteristics, magnetic behavior. Here, recent progress is reviewed, focusing on potential these for electrochemical, catalysis, biomedical applications advantages over conventional symmetric materials. Meanwhile, a variety strategies including template method, nanoemulsion assembly self‐assembly described detail. In addition, contradictions between material synthesis application pointed out, limitations methods morphology modulation means, well trade‐off property improvement production costs. Finally, future development path envisioned, emphasizing importance close integration theory practice, looking forward promoting new generation high‐performance through in‐depth understanding design principles action mechanisms ACBMs.

Язык: Английский

Процитировано

0
Split crRNA Precisely Assisted Cas12a Expansion Strategy for Simultaneous, Discriminative, and Low-Threshold Determination of Two miRNAs Associated with Multiple Sclerosis DOI
Xinyi Xia,

Zhigang Liang,

Guanhong Xu

и другие.

Analytical Chemistry, Год журнала: 2025, Номер 97(5), С. 2873 - 2882

Опубликована: Фев. 3, 2025

Multiple sclerosis (MS) can proceed into secondary progressive MS accompanied by persistent neurological deterioration; therefore, accurate diagnosis of is vital significance. Irregularities microRNAs (miRNAs) expression have been observed in MS, so miRNAs evaluated as novel biomarkers and therapeutic targets. Herein, a new strategy named split crRNA precisely assisted Cas12a expansion (SPACE) was developed for simultaneous, discriminative, low-threshold determination two MS-related miRNAs: miRNA-155 miRNA-326. On the one hand, owing to property that could activate Cas12a, were designed spacers combine with scaffold. These integrated crRNAs then recognized activators, activating enabling RNA target identification. other SPACE dexterously activator reporter probe, utilized Cas12a′s cis-cleavage achieve simultaneous detection differential signal output Moreover, trans-cleavage ultra-high efficiency assembled sensitive quantification total blood samples at low thresholds. Overall, diversified design enabled dual pot step, providing reliable tool clinical diagnosis.

Язык: Английский

Процитировано

0
Dual-Emission Carbon Dots Prepared from Quinoline Quaternary Ammonium Salts with Multiple Modulations DOI
Man Zhang, Xiaoyan Xu, Zhongyu Li

и другие.

The Journal of Physical Chemistry C, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Язык: Английский

Процитировано

0
Applications of Green Carbon Dots in Personalized Diagnostics for Precision Medicine DOI Open Access

Habtamu Fekadu Etefa,

F.B. Dejene

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2846 - 2846

Опубликована: Март 21, 2025

Green carbon dots (GCDs) have emerged as a revolutionary tool in precision medicine, offering transformative capabilities for personalized diagnostics and therapeutic strategies. Their unique optical biocompatible properties make them ideal non-invasive imaging, real-time monitoring, integration with genomics, proteomics, bioinformatics, enabling accurate diagnosis tailored treatments based on patients’ genetic molecular profiles. This study explores the potential of GCDs advancing individualized patient care by examining their applications medicine. It evaluates utility diagnostic targeted therapy delivery, formulation treatment plans, emphasizing interaction advanced genomic, proteomic, bioinformatics platforms. demonstrated exceptional versatility precise delivering therapies. cutting-edge technologies showed significant promise crafting strategies, enhancing functionality effectiveness monitoring patient-specific applications. The findings underscore pivotal role reshaping healthcare medicine improving outcomes. ongoing development emerging to further enhance capabilities, paving way more effective, medical care.

Язык: Английский

Процитировано

0
NIR–II DNA Nanodevice for Spatiotemporal MicroRNAs Imaging Based on “and” Logic‐Gate Strategy DOI
Lei Li,

Baochun Zhang,

Yifan Wang

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

Abstract The precise detection of multiple microRNAs (miRNAs) is great significance to the classification various cell subtypes. However, miRNA hindered by signal leakage, false‐positive signals and low expression miRNAs. Herein, a DNA nanodevice in second near infrared region (NIR‐II, 1000‐1700 nm) (DND‐II) developed for dual miRNAs bioimaging based on “AND” logic‐gate amplification strategy. spatiotemporally controlled logic‐gate‐based DND‐II fabricated thermosensitive liposomes integrated with near‐infrared probes. In particular, “always‐on” photoacoustic probes provided an additional imaging tool monitor distribution vivo. When both are present, generated “turn on” fluorescent through logical analysis molecular computation, further triggering entropy‐driven dual‐cycle fluorescence amplification. spatiotemporal provides powerful platform early diagnosis photothermal ablation different subtypes tumor cells.

Язык: Английский

Процитировано

0