Analytical Chemistry,
Год журнала:
2024,
Номер
97(1), С. 602 - 612
Опубликована: Дек. 23, 2024
Nowadays,
optical
tweezers
play
a
vital
role
not
only
in
manipulation
but
also
bioassay.
As
principal
trapping
objects,
microbeads
can
combine
with
suspension
array
technology,
amply
focused
laser
beams
and
adequately
concentrated
tags
contributing
to
highly
sensitive
detection.
In
view
of
the
inefficiency
conventional
single-trap
tweezers,
multitrap
systems
are
developed.
Here,
green-
blue-emitting
core-shell-shell
upconversion
nanoparticles
(UCNPs)
adopted
encode
determine
dual
miRNAs,
internal
shells
leading
luminescence
process
facilitate
quenching
through
resonance
energy
transfer
(LRET).
Utilizing
trans
cleavage
CRISPR/Cas12a,
quenched
signals
recovered
amplified,
causing
further
enhanced
detection
sensitivity.
Ultimately,
limits
(LOD)
17
22
aM
obtained
excellent
specificities
verified.
Furthermore,
miRNAs
from
MCF-7,
A549,
MCF-10A
cells
extracted
detected,
results
consistent
those
by
PCR.
Notably,
miR-155
MCF-7
A549
is
detectable
at
single-cell
level.
Thus,
differences
measured
miRNA
levels
between
imply
potential
this
method
discriminate
breast
cancer
epithelial
despite
difficulty
distinguishing
different
due
similar
levels.
Chemical Society Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
By
integrating
four
energy
modalities—NIR
light,
radiation,
magnetic
field,
and
ultrasound—with
bioengineered
nanomaterials,
dynamic
in
vivo
diagnostics
can
be
achieved,
advancing
the
path
toward
personalized
precision
medicine.
Nanomaterials,
Год журнала:
2024,
Номер
14(16), С. 1326 - 1326
Опубликована: Авг. 7, 2024
Rare
earth-doped
nanoparticles
(RENPs)
are
promising
biomaterials
with
substantial
potential
in
biomedical
applications.
Their
multilayered
core-shell
structure
design
allows
for
more
diverse
uses,
such
as
orthogonal
excitation.
However,
the
typical
synthesis
strategies-one-pot
successive
layer-by-layer
(LBL)
method
and
seed-assisted
(SA)
method-for
creating
RENPs
show
notable
differences
spectral
performance.
To
clarify
this
issue,
a
thorough
comparative
analysis
of
elemental
distribution
characteristics
synthesized
by
these
two
strategies
was
conducted.
The
SA
strategy,
which
avoids
partial
mixing
stage
shell
core
precursors
inherent
LBL
produces
distinct
interface
distribution.
This
unique
reduces
unnecessary
energy
loss
via
transfer
between
heterogeneous
elements
different
layers.
Consequently,
choice
can
effectively
modulate
properties
RENPs.
discovery
has
been
applied
to
RENP
probes
appropriate
dimensions,
where
strategy
introduces
refined
inert
prevent
loss.
Notably,
exhibited
4.3-fold
enhancement
NIR-II
vivo
imaging
2.1-fold
increase
reactive
oxygen
species
(ROS)-related
photodynamic
therapy
(PDT)
Abstract
Upconversion
nanoparticles
(UCNPs)
have
attracted
considerable
interest
for
the
imaging
of
solid
tumors
because
their
unique
optical
features.
These
applications
can
be
expanded
towards
anticancer
therapeutics
by
developing
UCNP-graphene
oxide
(GO)
composites.
This
strategy
addresses
low
loading
capacity
and
poor
dispersibility
UCNPs
in
physiological
media.
aspects
been
covered
this
article.
We
begin
discussing
synthesis
methods
challenges
associated
with
UCNPs,
along
surface
modification
strategies.
Next,
we
describe
approaches
designing
UCNP-GO
composites
imaging,
biosensing,
different
therapeutic
platforms.
Graphical
Sensors,
Год журнала:
2024,
Номер
24(23), С. 7715 - 7715
Опубликована: Дек. 2, 2024
Food
safety
is
of
great
concern,
and
food-borne
bacterial
infections
diseases
are
a
major
crisis
for
health.
Therefore,
it
necessary
to
develop
rapid
detection
techniques
the
prevention
recognition
food
hazards
caused
by
pathogens.
In
recent
years,
fluorescence
assay
has
become
widely
utilized
method
due
its
good
signal
amplification
effect,
high
sensitivity,
stability,
short
time.
this
review,
different
kinds
materials
were
concentrated,
including
quantum
dots
(QDs),
carbon
(CDs),
metal-organic
frameworks
(MOFs),
upconversion
nanoparticles
(UCNPs).
The
optical
properties
applications
fluorescent
analyzed
compared.
Furthermore,
according
biosensing
components,
biosensors
reviewed,
label-free
based
probes,
aptamer-based
biosensors,
antibody-based
biosensors.
Finally,
we
focused
our
attention
on
discussion
combined
with
other
their
applications.
review
presents
future
trends
in
sensors,
providing
new
sights
Analytical Chemistry,
Год журнала:
2024,
Номер
97(1), С. 602 - 612
Опубликована: Дек. 23, 2024
Nowadays,
optical
tweezers
play
a
vital
role
not
only
in
manipulation
but
also
bioassay.
As
principal
trapping
objects,
microbeads
can
combine
with
suspension
array
technology,
amply
focused
laser
beams
and
adequately
concentrated
tags
contributing
to
highly
sensitive
detection.
In
view
of
the
inefficiency
conventional
single-trap
tweezers,
multitrap
systems
are
developed.
Here,
green-
blue-emitting
core-shell-shell
upconversion
nanoparticles
(UCNPs)
adopted
encode
determine
dual
miRNAs,
internal
shells
leading
luminescence
process
facilitate
quenching
through
resonance
energy
transfer
(LRET).
Utilizing
trans
cleavage
CRISPR/Cas12a,
quenched
signals
recovered
amplified,
causing
further
enhanced
detection
sensitivity.
Ultimately,
limits
(LOD)
17
22
aM
obtained
excellent
specificities
verified.
Furthermore,
miRNAs
from
MCF-7,
A549,
MCF-10A
cells
extracted
detected,
results
consistent
those
by
PCR.
Notably,
miR-155
MCF-7
A549
is
detectable
at
single-cell
level.
Thus,
differences
measured
miRNA
levels
between
imply
potential
this
method
discriminate
breast
cancer
epithelial
despite
difficulty
distinguishing
different
due
similar
levels.
