TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 118121 - 118121
Опубликована: Дек. 1, 2024
Язык: Английский
Lab on a Chip, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Centrifugal microfluidics, with its advantages of rapid and precise fluid control without the need for external pressure, is widely applied in point-of-care testing.
Язык: Английский
Процитировано
1
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Фев. 8, 2025
Tailored droplet generation is crucial for microfluidics that involve samples of varying sizes. However, the absence precise predictive models forces platforms to rely on empiricism derived from extensive experiments, underscoring need comprehensive modeling analysis. To address this, a novel customized assembled centrifugal step emulsifier (CASE) presented by incorporating "jigsaw puzzles" design efficiently acquire large-scale experimental data. Numerical simulations are utilized analyze fluid configurations during emulsification, identifying key connection tube determines size. By training and verifying with simulation datasets, theoretical model established allows preliminary size frequency an average error rate 4.8%, successfully filling critical gap in existing field. This empowers CASE achieve all-in-one functionality, including pre-design, generation, manipulation, on-site detection. As proof concept, multiscale sample analysis ranging nanoscale nucleic acids microscale bacteria 3D cell spheroids realized CASE. In summary, this platform offers valuable guidance emulsifiers promotes adoption biochemical assays.
Язык: Английский
Процитировано
1
Talanta, Год журнала: 2025, Номер 291, С. 127815 - 127815
Опубликована: Фев. 27, 2025
Язык: Английский
Процитировано
1
Talanta, Год журнала: 2025, Номер 287, С. 127619 - 127619
Опубликована: Янв. 23, 2025
Язык: Английский
Процитировано
0
Sensors, Год журнала: 2025, Номер 25(6), С. 1936 - 1936
Опубликована: Март 20, 2025
Microfluidic biosensors integrate microfluidic and biosensing technologies to achieve the miniaturization, integration, automation of disease diagnosis, show great potential for application in fields cancer liquid biopsy, pathogenic bacteria detection, POCT. This paper reviews recent advances related field laboratory medicine, focusing on their applications above three areas. In facilitate isolation, enrichment, detection tumor markers such as CTCs, ctDNA, miRNA, exosomes, so on, providing support early precise treatment, prognostic assessment. terms can rapid, highly sensitive, specific a variety bacteria, helping prevention control well public health safety. Pertaining realm POCT, bring convenient diseases, tumors, infectious chronic primary care. Future biosensor research will focus enhancing throughput, lowering costs, innovating new recognition elements signal transduction methods, integrating artificial intelligence, broadening include home care, drug discovery, food safety, on.
Язык: Английский
Процитировано
0
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Март 31, 2025
Abstract The early detection of high‐risk human papillomavirus (HR‐HPV) is crucial for the assessment and improvement prognosis in cervical cancer. However, existing PCR‐based screening methods suffer from inadequate accessibility, which dampens enthusiasm among grassroots populations, especially resource‐limited areas, contributes to persistently high mortality rate Here, a portable system proposed multiplexed nucleic acid detection, termed R‐CHIP , that integrates R ecombinase polymerase amplification (RPA), C RISPR H and‐driven microfluidics, an artificial I ntelligence P latform. can go sample pre‐processing results readout less than hour with simple manual operation. Optimized sensitivity 10 −17 M HPV‐16 −18 HPV‐18, has accuracy over 95% 300 tests on clinical samples. In addition, smartphone microimaging combined ResNet‐18 deep learning model used improve efficiency convenience system, initial prediction accuracies 96.0% 98.0% respectively. R‐CHIP, as user‐friendly intelligent platform, great potential community‐level HR‐HPV resource‐constrained settings, prevention diagnosis other diseases.
Язык: Английский
Процитировано
0
JACS Au, Год журнала: 2025, Номер 5(2), С. 536 - 549
Опубликована: Фев. 12, 2025
Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb), with a complex pathogenesis that poses long-term threat to human health globally. Early and accurate diagnosis of TB provides critical window for timely effective treatment. The development nucleic acid testing (NAT) based on polymerase chain reaction (PCR) has greatly improved the diagnostic efficiency TB. However, balancing detection accuracy, efficiency, cost in NAT remains challenging. Functionalized nanomaterials-based nanobiosensors have demonstrated exceptional performance detecting integrating their unique physicochemical properties diverse biological probes exploit Mtb characteristics effectively amplify signals. Compared traditional NAT, simplify detection, improve reduce reliance external conditions, thereby contributing more immediate diagnosis. In this perspective, we provide comprehensive summary discussion current strategies biomarkers using along novel solutions Additionally, explore advantages challenges associated applying nanotechnology clinical management TB, particularly point-of-care (POCT).
Язык: Английский
Процитировано
0
TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118236 - 118236
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 19, 2025
The wash-free method represents a promising strategy for enhancing the detection efficiency of automated chemiluminescent (CL) immunoassay analyzers. Herein, novel separation-free and was developed first time based on distance-driven CL technique. In immunoassay, well-designed Au–Co metal nanoclusters (NCs) exhibited excellent peroxidase-like activity good stability, allowing efficient catalysis luminol or its analogue (ABEI)-H2O2 system even at low concentrations. Furthermore, large specific surface area NCs facilitated accommodation greater number antibodies, thereby capture antigens achieving dual amplification signal. technique relied formation sandwich-type immunocomplexes. Upon generation hydroxyl radicals superoxide anion through catalytic decomposition H2O2 by NCs, ABEI within immunocomplexes could efficiently react with these radicals, leading to significant enhancement in signals. C-reactive protein (CRP) chosen as model analyte evaluate practicability proposed immunoassay. Notably, presented high sensitivity, selectivity, reproducibility, successfully determining CRP serum samples recoveries 96.55–106.29%. Accordingly, advantage separation-free, wash-free, reliable characteristics drastically simplify operation steps enhance efficiency, which would make advances revolution traditional
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2024, Номер unknown
Опубликована: Сен. 13, 2024
Discs and numerous other consumer products have been developed for point of care testing (POCT) to replace traditional large expensive biochemical devices in certain scenarios. Herein, we propose a drip-dry strategy (2D strategy) assisted Blu-ray disc (BD) biosensor, termed BDB, rapid portable POCT within 30 min with the cost single test < $1. The platform utilizes covered area formed by deposition substance be measured on activated BD surface after evaporation water realizes quantitative detection target through error readout free quality diagnosis software. As proof concept, first demonstrated feasibility direct substances solution system pure proteins avoiding colorimetric reagent used optical detection. For complex mixed systems, then innovatively utilize principle that soluble targets promote/inhibit dissolution insoluble precipitates achieve specific successfully apply BDB indirect glutathione (GSH) LOD 0.447 mM range 2-16 organophosphorus pesticides (OPs) 2.122 × 10
Язык: Английский
Процитировано
0