Nanoscale, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Recent advancements in science and technology have significantly enhanced public health by integrating novel materials early diagnostic methods.
Язык: Английский
Microchimica Acta, Год журнала: 2025, Номер 192(4)
Опубликована: Март 5, 2025
The review article provides a short introduction to exosomes with the focus use as disease markers itself (i.e. their concentration or presence of some specific receptors) source biomarkers such proteins and metabolites. In detail, we are discussing various methods exosome isolation main paper is on affinity capture exosomes, since them can be applied sub-populations produced by organs. comprehensive overview magnetic (bio)affinity detection exosomal cargo using different ligands antibodies, DNA aptamers, peptides, glycan-based recognition, transferrin-based approaches, based recognition phospholipids other approaches including electrostatic interactions. in detail key analytical clinical parameters form an extensive table summarising outcomes published last two years (2023-2024). Finally, also conclusions sections pros cons for and/or determination content.
Язык: Английский
Процитировано
2
ACS Sensors, Год журнала: 2025, Номер unknown
Опубликована: Янв. 19, 2025
Fluorescence sensing is widely used in vitro detection due to its high sensitivity and rapid result delivery. However, systems based on nanomaterials involving complex cumbersome purification steps can lead sample loss significantly reduce the accuracy of results. To address this issue, we proposed a lanthanide-based aptasensor featuring target-triggered antenna effect enhance time-resolved luminescence (TRL) chelated Tb3+ combined with wash-free strategy. This sensor enabled highly sensitive epithelial cell adhesion molecule (EpCAM) exosomes from tumors, eliminating necessity for thereby delivering more stable reliable Specifically, using computer-aided design, sequence capable self-folding into hairpin structure (Antenna-Hairpin-Tb) was obtained. After hybridization an EpCAM-specific aptamer (AptEpCAM), duplex (Probe-Tb) constructed detection. When targeted binding occurred between AptEpCAM exosomes, Antenna-Hairpin-Tb dehybridized Probe-Tb underwent self-folding, leading significant sensitized TRL signal effect-induced energy transfer ligand Tb3+. Over broad range exosome concentrations (spanning 6.19 × 102 108 particles/mL), emission intensity at 543 nm linearly increased concentration (linear relationship: Y = 332.62 lg(Nexosomes) + 3690.5, R2 0.9956), achieving theoretical limit as low 17 particles/mL buffer. Furthermore, swift effective differentiation all cancer serum samples nonepithelial ones within just 30 min operation, 100% sensitivity, specificity, accuracy.
Язык: Английский
Процитировано
0
Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113477 - 113477
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(4), С. 235 - 235
Опубликована: Март 28, 2025
Cancer is a significant global health challenge, where early diagnosis crucial for enhancing patient survival and mitigating the treatment burden on patients. Exosomes are extracellular vesicles released through fusion of multivesicular bodies with cell membranes, carrying disease-associated information from donor cells. This makes exosomes key biomarker in liquid biopsy analysis, particularly cancer detection. Developing cost-effective, straightforward, sensitive exosome biosensing technologies practical importance. To date, large number fluorescence-based biosensors have relied Fluorescence Resonance Energy Transfer (FRET) principle. review introduces basic background field principle FRET-based sensors, followed by systematic summary their progress categorized different transduction elements or mechanisms. Finally, this work discusses current challenges proposes potential solutions future prospects, aiming to encourage inspire development new approaches advanced FRET biosensors.
Язык: Английский
Процитировано
0
ChemElectroChem, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Herein, the basic properties, composition, and isolation methods for separation of exosomes are described, since they can be a rich source disease biomarkers. Then an introduction to MXenes, novel class 2D nanomaterials with interesting properties applicable in numerous fields including biosensing is provided. Also, aptamers described as alternative antibodies robust biorecognition analytes interest. The final part article gives examples which these three key components integrated sensitive and/or electrochemical detection exosomes. conclusion provides summary initial achievements also outlook future discoveries exosome aptasensing using advanced nanomaterials, i.e., MXenes. MXenes have promising affinity‐based biosensing, being hydrophilic surface functional groups. In addition, free plasmons present used covalent grafting elements diazonium moieties. This especially approach immobilization DNA/RNA aptamers, readily modified by
Язык: Английский
Процитировано
0
Chinese Journal of Chromatography, Год журнала: 2025, Номер 43(5), С. 434 - 445
Опубликована: Май 1, 2025
Exosomes are nanoscale vesicles wrapped in lipid bilayers that secreted by cells and carry a variety of proteins, lipids, nucleic acids, metabolites. widely present various bodily fluids mediate intercellular communication. They participate physiological pathological processes, including immune regulation, angiogenesis, tumorigenesis, metastasis, have significant clinical diagnosis treatment potential. source-rich, structurally stable, reflect the states their parental cells. Therefore, they expected to serve as novel diagnostic markers for diseases. In addition, stem-cell-derived exosomes show therapeutic potential advantages low immunogenicity, high safety easy storage, exhibit neurodegenerative disorder, cardiovascular disease, cancer. Furthermore, highly biocompatible, natural homing properties, capable easily penetrating biological barriers, making them excellent drug-delivery carriers. Isolation enrichment is prerequisite downstream analysis application. High-purity, high-yield, high-throughput exosome-isolation methods be used applications. Based on physicochemical properties exosomes, density, size, charge, surface composition, mainly divided into density-based (e.g., differential ultracentrifugation, density-gradient ultracentrifugation), size-based ultrafiltration, size-exclusion chromatography, field-flow fractionation), polymer-precipitation polyethylene-glycol-based precipitation), chemical affinity antibody-based, aptamer-based, surface-lipid-based probes) methods. Currently, basic research applications face number challenges. Firstly, complexity heterogeneity lack standardized isolation has led variable results hinder comparing reproducing between different laboratories settings. Current generally hindered insufficient purity, yield, throughput, difficulties separating specific subpopulations, which seriously restrict development exosome field. Secondly, use clinic, few technical requirements, efficient inexpensive lacking. Commonly classical methods, such time-consuming, labor-intensive, require large sample volumes, inappropriate Methods immunoaffinity can isolate from precious trace samples practice; however, costs, recoveries, operating requirements shortcomings clinic. robust large-scale preparing There an urgent need develop repeatable scalable batches high-quality owing rapid Generally, progress greatly improve our understanding functions components will help transform effective strategies lead new precision-medicine personalized-treatment This article summarizes latest -enrichment technologies introduces application disease markers, agents, drug delivery Finally, future developmental trends discussed.
Язык: Английский
Процитировано
0
Microchemical Journal, Год журнала: 2025, Номер unknown, С. 114010 - 114010
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Nanoscale, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Recent advancements in science and technology have significantly enhanced public health by integrating novel materials early diagnostic methods.
Язык: Английский
Процитировано
0