Programmable PCR-like Nonenzymatic DNA Molecular Circuit for Split-Free Autocatalytic Amplification DOI Creative Commons

Ting Li,

Tat‐San Lau, Junyou Li

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 29, 2025

Nonenzymatic autocatalytic DNA circuits, capable of exponential signal amplification, exhibit superior amplification efficiency compared to traditional single or cascade which offer only linear quadratic amplification. However, circuits are currently limited by complicated splitting designs, low efficiency, the need for additional probes, and a lack universal design principles. Herein, we developed PCR-like split-free (SAA) circuit with simple but extraordinary effective biosensing. The SAA system could consecutively perform multiple cycles three-step process upon initiation, target recognition, replicating, recycling, is programmed mimic basic three reaction steps PCR constantly yield numerous new replicates expedite whole reaction, ultimately producing an exponentially amplified signal. sigmoidal kinetics, ability accurately execute instructions, high capability demonstrated, achieves same replication as without enzymes precise control temperature. circuit, characterized design, minimal components, offers promising approach developing highly efficient circuits. This enables analysis low-abundance biomarkers leakage, holding significant potential biochemical research clinical diagnosis.

Язык: Английский

Photoexcited Electro‐Driven Reactive Oxygen Species Channeling for Precise Extraction of Biomarker Information from Tumor Interstitial Fluid DOI Open Access
Xiyue Xie,

Shuqi Tang,

Chunhui Zhai

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

Direct electrochemical detection of miRNA biomarkers in tumor tissue interstitial fluid (TIF) holds great promise for adjuvant therapy tumors the perioperative period, yet is limited by background interference and weak signal. Herein, a wash-free separation-free biosensor based on photoexcited electro-driven reactive oxygen channeling analysis (LEOCA) developed to solve high-fidelity physiological samples. In presence miRNA, nanoacceptors (ultrasmall-size polydopamine, uPDA) are responsively assembled surface nanodonors (zirconium metal-organic framework, ZrMOF) form core-satellite aggregates. The produced lifetime-constraint singlet upon light irradiation captured catechol constrained uPDA, oxidized quinone immediately electro-reduced at transient collision process electrode, resulting cascade electron transfer amplified current. Thereby, nanosensor exhibits low limit (1.1 fM), high reproducibility (relative standard deviation 2.0%). Compared with quantitative real-time polymerase chain reaction (qRT-PCR), clinical accuracy (area under curve value) significantly increased from 0.75 0.93 distinguishing breast cancer patients healthy donors. This study demonstrates an inspiration synergy between nanodonor/nanoacceptor synchronous electrode bottleneck problem detecting unprocessed samples sample-in-answer-out manner.

Язык: Английский

Процитировано

0
A DNA-Based Plasmonic Nano-Ruler DOI Open Access
Aura Cencini,

Mary Bortoluzzi,

Graziano Rilievo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2557 - 2557

Опубликована: Март 12, 2025

DNA is an exceptional building block for the fabrication of dynamic supramolecular systems with switchable geometries. Here, a self-assembled, tunable plasmonic–fluorescent nanostructure was developed. A precise sliding motion mechanism operated through control strand displacement reactions, shifting two single-strand (ssDNA) rails connected by ssDNA quasi-ring structure. The system reconfigured as nano-mechanical structure, generating six discrete configurations, and setting specific distances between tethered gold nanoparticle (AuNP) fluorophore, Sulfo-Cyanine3 (Cy3). Each configuration produced distinct fluorescence emission intensity via plasmonic quenching/enhancement effects, therefore structure behaved nano-ruler. To optimize system, reversible distance-dependent quenching or enhancement phenomena were investigated testing AuNPs diameters 5, 10, 15 nm, yielding best performances 10 nm AuNPs. Furthermore, geometric model produced, confirming observed results. fluorophore–plasmonic surface positioning, conferred ruler, led to finite state nano-machine alternative signal outputs. This mechanism, working fluorescent reporter, could find application in multiple-responsive detection nucleic acids, such viruses microRNAs.

Язык: Английский

Процитировано

0
Visualization of Cancer Cell-Derived Exosome-Induced Fibroblast Phenotypic Transformation DOI

Shuhang Yin,

Feng Chen, Bi Peng

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Fibroblast phenotypic transformation plays a crucial role in tumorigenesis. Tumor cell-derived exosome-mediated fibroblast has been commonly studied. However, there is still no direct evidence that the process of tumor exosomes induces fibroblasts into cancer-associated (CAFs). Herein, exosomal miRNAs were used as target to track invasion cancer normal fibroblasts, and FAP mRNA, CAF biomarker, further trace effect on recipient cells. Two DNA tetrahedral sensors developed based hybrid chain reaction signal amplification strategy fluorescence resonance energy transfer mechanism. Both showed high sensitivity good selectivity, with detection limits 4.94 6.61 pM for miR-1247-3p respectively. Moreover, two allowed us image living cell postinvasion tumor-derived exosomes, facilitating visual monitoring phenotype uncovering new perspectives metastasis development.

Язык: Английский

Процитировано

0
Imaging methods to monitor and quantify cell differentiation DOI Creative Commons

Kse Cheah,

Pingjin Chu,

Georg Schmidt

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Май 13, 2025

The transition of a cell from stem to differentiated state involves an interrelated and complex series events. These events include dynamic changes in cellular nucleic acid protein content that are mediated by both intrinsic extrinsic factors which ultimately lead differentiation into specific lineage. Quantifying the parameters associated with their under different conditions would not only allow for better understanding this process but also enable development approaches control differentiation. Here, we describe processes two types cultured cells, neurons fibroblasts, tools follow real time. Specifically, discuss methods identify lineage, morphology, shifts mRNA miRNA levels as well localization, interactions assemblies accompany

Язык: Английский

Процитировано

0
Programmable PCR-like Nonenzymatic DNA Molecular Circuit for Split-Free Autocatalytic Amplification DOI Creative Commons

Ting Li,

Tat‐San Lau, Junyou Li

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 29, 2025

Nonenzymatic autocatalytic DNA circuits, capable of exponential signal amplification, exhibit superior amplification efficiency compared to traditional single or cascade which offer only linear quadratic amplification. However, circuits are currently limited by complicated splitting designs, low efficiency, the need for additional probes, and a lack universal design principles. Herein, we developed PCR-like split-free (SAA) circuit with simple but extraordinary effective biosensing. The SAA system could consecutively perform multiple cycles three-step process upon initiation, target recognition, replicating, recycling, is programmed mimic basic three reaction steps PCR constantly yield numerous new replicates expedite whole reaction, ultimately producing an exponentially amplified signal. sigmoidal kinetics, ability accurately execute instructions, high capability demonstrated, achieves same replication as without enzymes precise control temperature. circuit, characterized design, minimal components, offers promising approach developing highly efficient circuits. This enables analysis low-abundance biomarkers leakage, holding significant potential biochemical research clinical diagnosis.

Язык: Английский

Процитировано

0