Dual‐Mode Detection of RNA Demethylation Protein ALKBH3 Using a Two‐Legged DNA‐Walker‐Mediated BiOI/In2O3‐Gated Organic Photoelectrochemical Transistor Biosensor DOI
Yixin Hu, Yunlei Zhou, Huanshun Yin

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

Abstract Organic photoelectrochemical transistor (OPECT) biosensors have emerged as prevalent candidates for bioanalytical technologies because of their intrinsic amplification properties and excellent detection performance. Herein, a novel OPECT biosensor analyzing the RNA demethylation protein ALKBH3 is developed. A BiOI/In 2 O 3 type‐II heterojunction employed photoactive material gate, an organic semiconductor, poly(3,4‐ethylenedioxythiophene): poly(styrenesulfonic) (PEDOT:PSS), used channel modification material. ALKBH3‐mediated demethylation, switch, triggered trans‐cleavage activity CRISPR‐Cas12a system, which subsequently leads to failure in formation bipedal DNA walker. Contrarily, ferrocenecarboxylic acid (FcA), acts effective electron donor, cannot be captured, leading modulation gating characteristics degradation current response. Compared conventional method, exhibits higher sensitivity lower limit protein, thereby facilitating monitoring changes under effect environmental pollutants demonstrating crucial biomarker potential evaluating its toxicological effects.

Язык: Английский

Enhanced carrier separation and decreased reaction barrier in cobalt-doped CdIn2S4 nanosheets for photocatalytic hydrogen evolution DOI
Liang Mao,

Qinran Li,

Qing Zhou

и другие.

Journal of Colloid and Interface Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Efficient Al–H3NTB-MOG ECL Emitter with Self-Enhanced and AIECL Performance for Ultrasensitive Sensing of miRNA-141 Combined with a Y-Shaped Multiregion Dual-Drive DNA Walker DOI
Jiping Yang, Li Wang, Yifei Chen

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

In this work, using Al-H3NTB-MOG with self-enhanced and aggregation-induced electrochemiluminescence (AIECL) performance as an efficient emitter, a biosensor based on Y-shaped multiregion dual-drive DNA walker was constructed for the sensitive detection of miRNA-141. Notably, 4,4',4″-nitrilotribenzoic acid (H3NTB) selected luminescent ligand ECL property co-reactive tertiary amine in its structure. Al3+ served central ion to coordinate H3NTB form three-dimensional porous gel structure, which restricted internal rotation vibration benzene molecules exhibited excellent AIECL property. More interestingly, N-2-hydroxyethylpiperazine-N'-ethane-sulfonic (HEPES) chosen system buffer, could not only stabilize test environment but also play co-reaction compensation role compensate consumption groups process, then significantly resulting better stability response. Besides, dynamic signal amplification established synergistic effect rolling cycle (RCA) process ionic cleavage at both ends nanostructure assembled by catalytic hairpin self-assembly (CHA) reaction. Specifically, two long chains abundant recognition regions were formed RCA reaction walker, simultaneously walk along predesigned tracks shear specific sites from directions, effectively improving efficiency. that way, realized miRNA-141 10 aM 1 nM range limit low 6.48 aM.

Язык: Английский

Процитировано

0
Dual‐Mode Detection of RNA Demethylation Protein ALKBH3 Using a Two‐Legged DNA‐Walker‐Mediated BiOI/In2O3‐Gated Organic Photoelectrochemical Transistor Biosensor DOI
Yixin Hu, Yunlei Zhou, Huanshun Yin

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

Abstract Organic photoelectrochemical transistor (OPECT) biosensors have emerged as prevalent candidates for bioanalytical technologies because of their intrinsic amplification properties and excellent detection performance. Herein, a novel OPECT biosensor analyzing the RNA demethylation protein ALKBH3 is developed. A BiOI/In 2 O 3 type‐II heterojunction employed photoactive material gate, an organic semiconductor, poly(3,4‐ethylenedioxythiophene): poly(styrenesulfonic) (PEDOT:PSS), used channel modification material. ALKBH3‐mediated demethylation, switch, triggered trans‐cleavage activity CRISPR‐Cas12a system, which subsequently leads to failure in formation bipedal DNA walker. Contrarily, ferrocenecarboxylic acid (FcA), acts effective electron donor, cannot be captured, leading modulation gating characteristics degradation current response. Compared conventional method, exhibits higher sensitivity lower limit protein, thereby facilitating monitoring changes under effect environmental pollutants demonstrating crucial biomarker potential evaluating its toxicological effects.

Язык: Английский

Процитировано

0