Manganese-Based Nanotherapeutics for Targeted Treatment of Breast Cancer

ACS Applied Bio Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Breast cancer (BC) is one of the most common cancers among women and associated with high mortality. Traditional modalities, including surgery, radiotherapy, chemotherapy, have achieved certain advancements but continue to combat challenges harm healthy tissues, resistance treatment, adverse drug reactions. The rapid in nanotechnology recently facilitated exploration innovative strategies for breast therapy. Manganese-based nanotherapeutics attracted great attention because their unique characteristics such as tunable structures/morphologies, versatility, magnetic/optical properties, strong catalytic activities, excellent biodegradability, biocompatibility. In this review, we highlighted different types Mn-based modulate TME, metal-immunotherapy, alleviating tumor hypoxia, increasing reactive oxygen species production, emphasized its role magnetic resonance imaging (MRI)-guided therapy, photoacoustic imaging, theranostic-based therapy along a therapeutic carrier, all which were discussed context cancer. Hopefully, present review will provide insights into current landscape future directions multifunctional applications field treatment.

Язык: Английский

Cross-Species Multi-Omics Analysis Reveals Myeloid-Driven Endothelial Oxidative Stress in Ischemic Stroke

Frontiers in Bioscience-Landmark, Год журнала: 2025, Номер 30(4)

Опубликована: Апрель 16, 2025

Background: Ischemic stroke is a leading cause of mortality and disability worldwide, yet the interplay between peripheral central immune responses still only partially understood. Emerging evidence suggests that myeloid cells, when activated in periphery, infiltrate ischemic brain contribute to disruption blood-brain barrier (BBB) through both inflammatory metabolic mechanisms. Methods: In this study, we integrated bulk RNA-sequencing (RNA-seq), single-cell RNA-seq (scRNA-seq), spatial transcriptomics, flow cytometry data from human mouse models stroke. Mouse were induced by transient middle cerebral artery occlusion (tMCAO), tissues later collected at specified time points for analysis. We examined time-dependent transcriptional changes blood, delineated cell-type-specific profiling, validated infiltration into brain. also investigated endothelial reprogramming oxidative stress combining scMetabolism analyses (a computational R package inferring pathway activity level) with vitro oxygen-glucose deprivation/reperfusion (OGD/R) experiments. Results: Cross-species revealed modest early shift 3 h post-stroke, escalating significantly 24 h, robust myeloid-centric gene signatures conserved humans mice. Single-cell confirmed pronounced expansion neutrophils, monocytes, megakaryocytes coupled decrease T B lymphocytes. Spatial transcriptomics demonstrated substantial CD11b+ cells infarct core, which showed extensive interaction cells. Endothelial scRNA-seq reductions phosphorylation, glutathione, nicotinate pathways, together elevated pentose phosphate activity, suggestive compromised antioxidant capacity. Functional scoring further indicated diminished inflammation/repair potential, while OGD/R experiments morphological disruption, CD31 downregulation, increased 4-hydroxynonenal (4-HNE), underscoring importance damage BBB breakdown. Conclusions: These multi-omics findings highlight existence coordinated peripheral-central axis stroke, wherein cell recruitment vulnerability jointly exacerbate inflammation stress. The targeting injury myeloid-endothelial crosstalk may represent promising strategy mitigate secondary

Язык: Английский

Mesenchymal Stem-Cell-Derived Exosomes Loaded with Phosphorus Dendrimers and Quercetin Treat Parkinson’s Disease by Modulating Inflammatory Immune Microenvironment

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

The intricate pathologic features of Parkinson's disease (PD) coupled with the obstacle posed by blood-brain barrier (BBB) significantly limit efficacy most medications, leading to difficulties in PD treatments. Herein, we have developed a nanomedicine based on stem-cell-derived exosomes coloaded hydroxyl-terminated phosphorus dendrimers (AK76) and quercetin (Que) for combined therapeutic intervention PD. engineered nanocomplexes (for short, QAE NPs) exhibit an optimal size 269.7 nm, favorable drug release profile, desired cytocompatibility, enabling penetration nasal mucosa accumulate brain without BBB crossing. NPs can scavenge reactive oxygen species, promote M2 microglial polarization, attenuate inflammation, protect neurons inducing autophagy restoring mitochondrial homeostasis through integrated anti-inflammatory antioxidant properties exosomes, Que AK76, collectively improved motor functions, coordination, alleviation depression-like symptoms mice. formulated several components are able simultaneously modulate both microglia neurons, offering promising potential treatment other neurodegenerative disorders.

Язык: Английский