Bioprocess and Biosystems Engineering, Год журнала: 2025, Номер unknown
Опубликована: Июнь 5, 2025
Язык: Английский
Micromachines, Год журнала: 2024, Номер 15(7), С. 932 - 932
Опубликована: Июль 22, 2024
Microfluidic separators play a pivotal role in the biomedical and chemical industries by enabling precise fluid manipulations. Traditional fabrication of these devices typically requires costly cleanroom facilities, which limits their broader application. This study introduces novel microfluidic device that leverages passive Zweifach–Fung principle to overcome financial barriers. Through Lagrangian computational simulations, we optimized an eleven-channel configuration achieved perfect 100% recall rate for particles following specified normal distribution. Experimental evaluations determined 2 mL/h as optimal total flow (TFR), under showcased exceptional performance enhancements precision micrometer-sized particles, achieving overall accuracy 94% ± 3%. Fabricated using cost-effective, non-cleanroom method, this approach represents significant shift from conventional practices, dramatically reducing production costs while maintaining high operational efficacy. The cost each chip is less than USD 0.90 cents manufacturing process takes only 15 min. development not makes technology more accessible but also sets new standard future advancements field.
Язык: Английский
Процитировано
10
ACS Sensors, Год журнала: 2025, Номер unknown
Опубликована: Янв. 27, 2025
To enhance exploration on tumor stem-like cells (TSCs) without altering their cellular biological characteristics, researchers advocate for application of single-cell-derived tumor-spheres (STSs). TSCs are regulated by surrounding microenvironment, making it crucial to simulate a microenvironment facilitate STS formation. Recently, exosomes that originated from the have emerged as promising approach mimicking microenvironment. In various associated (such fibroblasts, endothelial cells, and immune cells) play roles. Utilizing derived these enabled us promote Herein, we developed an integrated microfluidic platform generate serial concentration gradients evaluate effects multiple demonstrate feasibility our approach, generated two different cell types (HUVEC NIH/3T3 assessed Subsequently, investigated drug resistance STSs treated with free doxorubicin doxorubicin-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles. Our findings revealed mixed could be successfully generated, leading enhanced formation rate size STSs. Compared one type, exhibited superior promotion Additionally, nanomedicines demonstrated reduction in compared treatment, particularly smaller and/or more deformable TSCs. This provides innovative enhancement simulation.
Язык: Английский
Процитировано
1
Advanced Healthcare Materials, Год журнала: 2025, Номер unknown
Опубликована: Фев. 24, 2025
Abstract More recently, single‐cell encapsulation emerged as a promising field in biomedicine due to its potential applications, cell analysis and therapy. Traditional techniques involve embedding cells crosslinked polymers create continuous microgels, suitable mainly for adherent cells, or encapsulating them droplets only short‐term analysis, their instability. In this study, we developed method single liquid‐core microcapsules address these limitations. The liquid system is generated an all aqueous environment through polymeric electrostatic interactions. Additionally, design innovative low cost sorting utilizing magnetic nanoparticles (MNPs) efficiently select encapsulated units further applications. This tested with both suspension types, demonstrating cytocompatibility no abnormal effects on behavior. MNP‐based achieved nearly 80% purity of the population. Overall, technology provides highly efficient such screening, by enabling precise short medium‐term real time monitoring, high resolution imaging cellular Furthermore, semipermeable membrane unlocks new advancing therapy offering protection while ensuring diffusion therapeutic factors, paving way strategies.
Язык: Английский
Процитировано
1
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 20, 2025
The isolation and detection of circulating tumor cells (CTCs) play a significant role in early cancer diagnosis prognosis. Negative magnetophoresis sorting is label-free method, providing easy access to enrich intact viable CTCs, but it struggles meet the demands high-throughput separation direct downstream analysis. In this work, facile cascaded negative microfluidic chip was fabricated online coupled inductively plasma mass spectrometry (ICP-MS) for rapid rare CTCs blood samples. consisted two parts: zone phase-transfer zone. zone, WBCs labeled with anti-CD45-magnetic beads (MBs) dispersed biocompatible ferrofluid were removed by magnetic attractive force, while anti-EpCAM-Eu migrated into repulsive force; due stable laminar flow formed fluid PBS buffer, under both force inertial lift introduced ICP-MS detection. This device can achieve CTC enrichment at high throughput 100 μL min-1 has capability analysis recultivation (cell viability 99.27%). method applied real clinical samples from 10 patients diagnosed various cancers, rate 100%, simple efficient approach CTCs.
Язык: Английский
Процитировано
1
Sensors and Actuators Reports, Год журнала: 2024, Номер unknown, С. 100277 - 100277
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
3
Journal of Magnetism and Magnetic Materials, Год журнала: 2025, Номер unknown, С. 172934 - 172934
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Results in Physics, Год журнала: 2025, Номер unknown, С. 108209 - 108209
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Journal of Fluid Mechanics, Год журнала: 2025, Номер 1008
Опубликована: Апрель 3, 2025
The acoustofluidic method holds great promise for manipulating micro-organisms. When exposed to the steady vortex structures of acoustic streaming flow, these micro-organisms exhibit intriguing dynamic behaviours, such as hydrodynamic trapping and aggregation. To uncover mechanisms behind we investigate swimming dynamics both passive active particles within a two-dimensional flow. By employing theoretically calculated flow field, demonstrate existence stable bounded orbits particles. Additionally, introduce rotational diffusion examine distribution under varying strengths. Our findings reveal that can laterally migrate across streamlines become trapped in closer centre, whereas are confined movement along streamlines. We emphasise influence field on particles, identifying configuration maximises their These insights contribute manipulation microswimmers development innovative biological microfluidic chips.
Язык: Английский
Процитировано
0
Biomicrofluidics, Год журнала: 2025, Номер 19(3)
Опубликована: Май 1, 2025
Acoustofluidics, offering contact-free and precise manipulation of micro-objects, has emerged as a transformative tool for various biological medical applications. In recent years, significant advancements have been made in droplet using acoustic waves. This review provides an in-depth exploration acoustofluidic techniques manipulation, presenting balanced perspective on the role this versatile platform across diverse The paper begins by introducing underlying mechanism forces acting droplets, followed comprehensive discussion tailored essential unit operations, such generation, separation, merging, splitting, steering, trapping, in-droplet sample control within sessile digital acoustofluidics. Finally, prospects limitations acoustofluidics manipulations are also discussed, suggesting future direction research.
Язык: Английский
Процитировано
0
Bioprocess and Biosystems Engineering, Год журнала: 2025, Номер unknown
Опубликована: Июнь 5, 2025
Язык: Английский
Процитировано
0