Bufalin Suppresses Head and Neck Cancer Development by Modulating Immune Responses and Targeting the β-Catenin Signaling Pathway DOI Open Access

Nour Mhaidly,

Noura Barake,

Anne Trelcat

и другие.

Cancers, Год журнала: 2024, Номер 16(15), С. 2739 - 2739

Опубликована: Авг. 1, 2024

Bufalin, a cardiotonic steroid derived from the Chinese toad (Bufo gargarizans), has demonstrated potent anticancer properties across various cancer types, positioning it as promising therapeutic candidate. However, comprehensive mechanistic studies specific to head and neck cancers have been lacking. Our study aimed bridge this gap by investigating bufalin’s mechanisms of action in cells. Using several methods, such Western blotting, immunofluorescence, flow cytometry, we observed dose-dependent reduction cell viability, disruption membrane integrity, inhibition colony formation both HPV-positive HPV-negative lines. Bufalin induces apoptosis through modulation apoptosis-related proteins, mitochondrial function, reactive oxygen species production. It also arrests cycle at G2/M phase attenuates migration while affecting epithelial–mesenchymal transition markers targeting pivotal signaling pathways, including Wnt/β-catenin, EGFR, NF-κB. Additionally, bufalin exerted immunomodulatory effects polarizing macrophages toward M1 phenotype, bolstering antitumor immune responses. These findings underscore potential multifaceted agent against cancers, essential pathways involved proliferation, apoptosis, regulation, metastasis, modulation. Further research is warranted validate these optimize clinical application.

Язык: Английский

Colorectal Cancer: Current and Future Therapeutic Approaches and Related Technologies Addressing Multidrug Against Multiple Level Resistance Mechanisms DOI Open Access

Marianna Puzzo,

Marzia De Santo,

Catia Morelli

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1313 - 1313

Опубликована: Фев. 4, 2025

Colorectal cancer (CRC) is the third most common and associated with a poor prognosis. The mutation profile related involved pathways of CRC have been, in broad terms, analyzed. main current therapeutic approaches been comprehensively reviewed here, future possible therapeu-tic options technologies perspectively presented. complex scenario represented by multiple-level resistance mechanism epidermal growth factor receptor (EGFR) pathway, including mutations KRAS, NRAS, BRAF V600E, discussed. Examples engineered from literature along drug combination tested clinical trials are encouraging results observed latter (the BEACON trial), totally free chemotherapy, prompted authors to imagine nanotechnology-assisted approach for bypassing mechanisms, hopefully allowing, principle, complete biological remission.

Язык: Английский

Процитировано

1
Prostate cancer chemotherapy by intratumoral administration of Docetaxel-Mesoporous silica nanomedicines DOI Creative Commons
Eva Rivero‐Buceta,

Adrián Bernal-Gómez,

Carla Vidaurre-Agut

и другие.

International Journal of Pharmaceutics, Год журнала: 2024, Номер 664, С. 124623 - 124623

Опубликована: Авг. 25, 2024

Docetaxel (DTX) is a recommended treatment in patients with metastasic prostate cancer (PCa), despite its therapeutic efficacy limited by strong systemic toxicity. However, localized PCa, intratumoral (IT) administration of DTX could be an alternative to consider that may help overcome the disadvantages conventional intravenous (IV) therapy. In this context, we here present first vivo preclinical study PCa therapy nanomedicines mesoporous silica nanoparticles (MSN) and IT injection over xenograft mouse model bearing human adenocarcinoma tumors. The tolerability, biodistribution histopathology after have been investigated for nanomedicine free drug, compared IV DTX. obtained results demonstrate allows precise selective non-metastatic minimize diffusion showing superior activity than route. This reducing dose one order widens substantially window drug. Furthermore, use as promotes antitumor drug accumulation at tumor site, improving same

Язык: Английский

Процитировано

4
Redefining Hepatocellular Carcinoma Treatment: Nanotechnology Meets Tumor Immune Microenvironment DOI
Chen Mi, Sai Liu, Zhida Chen

и другие.

Journal of drug targeting, Год журнала: 2025, Номер unknown, С. 1 - 47

Опубликована: Март 13, 2025

Hepatocellular carcinoma (HCC) is one of the most lethal malignancies worldwide, characterized by its complex pathogenesis and poor therapeutic outcomes. Despite recent advances in targeted molecular therapies, immune checkpoint inhibitors (ICIs), radiotherapy, conventional chemotherapy, five-year survival rate for this neoplasm remains dismally low. The progress nanotechnology has revolutionized cancer treatment years. These provide unprecedented opportunities to overcome current limitations different modalities. This review provides a comprehensive analysis how interfaces with tumor microenvironment (TIME) HCC can present new frontier interventions HCC. We critically overview latest developments nanoparticle-based delivery systems various drugs also other antitumor agents like thermal therapy radiotherapy. highlight unique ability nanoparticles modulate immunosuppressive (TME) enhance efficacy. Furthermore, we analyze emerging strategies that exploit nanoformulations biological barriers drug bioavailability treatment.

Язык: Английский

Процитировано

0
Design, Synthesis, and Evaluation of Cytotoxic Effects of Functional Fatty Acid Derivatives as Potential Antineoplastic Agents for Breast Cancer DOI Creative Commons
Maryam Hosseini, Farzad Kobarfard, Salimeh Amidi

и другие.

Deleted Journal, Год журнала: 2025, Номер 24(1)

Опубликована: Март 15, 2025

Background: Breast cancer is among the most prevalent cancers in women and leading cause of mortality worldwide. Although a definitive cure for breast remains elusive, essential fatty acids offer promising therapeutic avenue. Objectives: The present study aimed to synthesize 16 derivatives docosahexaenoic acid (DHA) linoleic (LA) evaluate their anti-cancer properties vitro. Methods: Fourteen LA DHA were synthesized using coupling method, while two ethylenediamine via an ester intermediate. Molecular modeling was conducted AutoDock Vina software. cytotoxic effects all compounds assessed MTT assay on adenocarcinoma (MCF-7) cells. mechanism cell death induction by with favorable EC50 values determined through annexin V-FITC/PI flow cytometry analysis, focusing early late apoptosis. Results: Docking results revealed that these effectively interact residues PTPB1 active site. All demonstrated MCF-7 line, no significant cytotoxicity observed normal human dermal fibroblasts (HDFs). Compounds D3 L3, 15.96 ± 2.89 μM 24.64 1.81 μM, respectively, identified as potent derivatives. Conclusions: findings indicate functional significantly reduce viability. In addition necrosis, L3 induced apoptosis, apoptosis rates 20.5% 47.1%, respectively.

Язык: Английский

Процитировано

0
A novel approach for the co-delivery of 5-fluorouracil and everolimus for breast cancer combination therapy: stimuli-responsive chitosan hydrogel embedded with mesoporous silica nanoparticles DOI Creative Commons
Pooria Mohammadi Arvejeh,

Fatemeh Amini Chermahini,

Francesco M. Marincola

и другие.

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Март 31, 2025

Abstract Background Breast cancer remains one of the leading causes death among women globally, with traditional therapies often limited by challenges such as drug resistance and significant side effects. Combination therapies, coupled nanotechnology-based co-delivery systems, offer enhanced efficacy targeting multiple pathways in progression. In this study, we developed an injectable, stimuli-responsive nanosystem using a chitosan hydrogel embedded mesoporous silica nanoparticles for co-administration 5-fluorouracil everolimus. This approach aims to optimize controlled release, enhance synergistic anticancer effect, overcome associated co-loading different therapeutic agents. Methods Various techniques were employed characterize hydrogel. Cell uptake, apoptosis, proliferation 4T1 breast cells evaluated flow cytometry Resazurin assay, respectively. The Balb/C mice model cancer, which received therapeutical nanoplatforms subcutaneously near tumoral region was used examine tumor size lung metastases. Results results revealed that had suitable loading capacity high cellular uptake. release pH-sensitive synergistic. By incorporating into hydrogel, cell rate apoptosis increased significantly, due effects co-delivered drugs. Additionally, combination treatment groups showed reduction metastasis compared monotherapy control groups. Conclusions These findings underscore potential nanocomposite develop novel system outcomes, reduce effects, provide promising new strategy future treatments.

Язык: Английский

Процитировано

0
Design, synthesis, and antiproliferative screening of new quinoline derivatives bearing a cis-vinyl triamide motif as apoptosis activators and EGFR-TK inhibitors DOI Creative Commons
Hany M. Abd El‐Lateef,

Ahmed Gaafar Ahmed Gaafar,

Arwa Sultan Alqahtani

и другие.

RSC Advances, Год журнала: 2024, Номер 14(34), С. 24781 - 24790

Опубликована: Янв. 1, 2024

A set of novel quinoline tethered cis -vinyl triamides derivatives has been designed, synthesized and screened for in vitro cytotoxicity against the MCF-7 breast adenocarcinoma cell line.

Язык: Английский

Процитировано

3
Nanotechnology in Advancing Chimeric Antigen Receptor T Cell Therapy for Cancer Treatment DOI Creative Commons
Xuejia Kang, Nur Mita, Lang Zhou

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(9), С. 1228 - 1228

Опубликована: Сен. 20, 2024

Chimeric antigen receptor (CAR) T cell therapy has emerged as a groundbreaking treatment for hematological cancers, yet it faces significant hurdles, particularly regarding its efficacy in solid tumors and concerning associated adverse effects. This review provides comprehensive analysis of the advancements ongoing challenges CAR-T therapy. We highlight transformative potential nanotechnology enhancing by improving targeting precision, modulating immune-suppressive tumor microenvironment, overcoming physical barriers. Nanotechnology facilitates efficient CAR gene delivery into cells, boosting transfection efficiency potentially reducing costs. Moreover, offers innovative solutions to mitigate cytokine release syndrome (CRS) immune effector cell-associated neurotoxicity (ICANS). Cutting-edge platforms real-time monitoring activity are also discussed. By integrating these advancements, we aim provide valuable insights pave way next generation therapies overcome current limitations enhance therapeutic outcomes.

Язык: Английский

Процитировано

2
Engineered Mesoporous Silica-Based Nanoparticles: Characterization of Surface Properties DOI Open Access
Antonio Grisolia,

Marzia De Santo,

Manuela Curcio

и другие.

Materials, Год журнала: 2024, Номер 17(13), С. 3352 - 3352

Опубликована: Июль 6, 2024

Mesoporous silica-based nanomaterials have emerged as multifunctional platforms with applications spanning catalysis, medicine, and nanotechnology. Since their synthesis in the early 1990s, these materials attracted considerable interest due to unique properties, including high surface area, tunable pore size, customizable chemistry. This article explores properties of a series MSU-type mesoporous silica nanoparticles, elucidating impact different functionalization strategies on characteristics. Through an extensive characterization utilizing various techniques, such FTIR, Z-potential, nitrogen adsorption porosimetry, insights into modifications nanoparticles are provided, contributing deeper understanding nanostructure related interactions, paving way possible unexpected actionability potential applications.

Язык: Английский

Процитировано

2
Bufalin Suppresses Head and Neck Cancer Development by Modulating Immune Responses and Targeting the β-Catenin Signaling Pathway DOI Open Access

Nour Mhaidly,

Noura Barake,

Anne Trelcat

и другие.

Cancers, Год журнала: 2024, Номер 16(15), С. 2739 - 2739

Опубликована: Авг. 1, 2024

Bufalin, a cardiotonic steroid derived from the Chinese toad (Bufo gargarizans), has demonstrated potent anticancer properties across various cancer types, positioning it as promising therapeutic candidate. However, comprehensive mechanistic studies specific to head and neck cancers have been lacking. Our study aimed bridge this gap by investigating bufalin’s mechanisms of action in cells. Using several methods, such Western blotting, immunofluorescence, flow cytometry, we observed dose-dependent reduction cell viability, disruption membrane integrity, inhibition colony formation both HPV-positive HPV-negative lines. Bufalin induces apoptosis through modulation apoptosis-related proteins, mitochondrial function, reactive oxygen species production. It also arrests cycle at G2/M phase attenuates migration while affecting epithelial–mesenchymal transition markers targeting pivotal signaling pathways, including Wnt/β-catenin, EGFR, NF-κB. Additionally, bufalin exerted immunomodulatory effects polarizing macrophages toward M1 phenotype, bolstering antitumor immune responses. These findings underscore potential multifaceted agent against cancers, essential pathways involved proliferation, apoptosis, regulation, metastasis, modulation. Further research is warranted validate these optimize clinical application.

Язык: Английский

Процитировано

0