Integrated 3D-Printed Hollow Microneedle Array and Lateral Flow Immunoassay for Point-of-Care Wound Healing Monitoring DOI
Amadeo Sena‐Torralba, Marc Parrilla,

Ana Hernanz-Grimalt

и другие.

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Chronic wound management requires continuous monitoring to assess healing and guide treatment. We developed a hollow microneedle array patch integrated with lateral flow immunoassay strip address the need for convenient, home-based diagnostics. This device extracts exudate directly from matrix, overcoming limitations of conventional swab sampling, which relies on surface collection. The provides minimally invasive, rapid solution phase. delivers colorimetric signal visible naked eye, facilitating straightforward interpretation by clinicians within 10 min. In clinical study involving 90 patients, matrix metalloproteinase 8 (MMP-8) was identified as critical biomarker, achieving 80.3% sensitivity in detecting proliferative A specific MMP-8 detection limit 0.7 ng/mL, lower than threshold level phase (164.7 ng/mL). 3D-printed cost-efficiency (less €0.10 per patch) height 865 ± 6 μm, allowing painless, easy sampling. Mechanical tests confirmed durability patch, while cytotoxicity assays demonstrated its biocompatibility. Prevalidation using an

Язык: Английский

Therapeutic Potential of Microneedle Assisted Drug Delivery for Wound Healing: Current State of the Art, Challenges, and Future Perspective DOI

Devesh Kumar,

Shubham Pandey,

Jailani Shiekmydeen

и другие.

AAPS PharmSciTech, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 8, 2025

Язык: Английский

Процитировано

4

Emerging Trends in Dissolving-Microneedle Technology for Antimicrobial Skin-Infection Therapies DOI Creative Commons
Rui Luo,

Huihui Xu,

Qiaoni Lin

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(9), С. 1188 - 1188

Опубликована: Сен. 8, 2024

Skin and soft-tissue infections require significant consideration because of their prolonged treatment duration propensity to rapidly progress, resulting in severe complications. The primary challenge stems from the involvement drug-resistant microorganisms that can form impermeable biofilms, as well possibility infection extending deep into tissues, thereby complicating drug delivery. Dissolving microneedle patches are an innovative transdermal drug-delivery system effectively enhances penetration through stratum corneum barrier, increasing concentration at site infection. They offer highly efficient, safe, patient-friendly alternatives conventional topical formulations. This comprehensive review focuses on recent advances emerging trends dissolving-microneedle technology for antimicrobial skin-infection therapy. Conventional antibiotic microneedles compared with those based agents, such quorum-sensing inhibitors, peptides, antimicrobial-matrix materials. also highlights potential incorporating chemodynamic, nanoenzyme antimicrobial, photodynamic, photothermal antibacterial therapies. explores advantages various therapies emphasizes combined application improve efficacy microneedles. Finally, this analyzes druggability different discusses possible future developments.

Язык: Английский

Процитировано

4

Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management DOI Creative Commons
Shiyang Lin, Zhongqi Cui,

Qingqiong Luo

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

Abstract The healing of bacterial biofilm‐infected wounds is a complex process, and the construction emerging therapeutic modalities that regulate microenvironment to magnify effects reduce biotoxicity still highly challenging. Herein, an engineered microneedle (MN) patch reported mediate efficient delivery black phosphorus nanosheets (BP NSs) copper peroxide nanodots (CP NDs) for dual nanodynamic sterilization methicillin‐resistant staphylococcus aureus (MRSA)‐infected wound healing. Results demonstrate system can eliminate biofilm, cytotoxicity, promote angiogenesis tissue regeneration by multiple advantages chemodynamic therapy (CDT), enhanced photodynamic (PDT), improved degradation process from BP NSs phosphate promoting cell proliferation. Notably, balance between excellent stability rapid degradability maintained, mechanism vividly elucidated density functional theory (DFT)‐based molecular dynamics (MD) calculations. Furthermore, transcriptional changes treated MRSA‐infected skin are studied using RNA‐seq technology reveal potential mechanism. As envisaged, proposed MN provides safe, easy, also effective approach achieve temporal regulation wounds.

Язык: Английский

Процитировано

0

Dissolving Microneedles Embedded with Photosensitizers for Targeted Eradication of Gram‐Positive Bacteria in Multidrug‐Resistant Biofilms in Diabetic Wound Infections DOI

Ruyan Xie,

Duoyang Fan,

Yanpeng Fang

и другие.

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Abstract Chronic nonhealing wounds, common in diabetic patients, represent a major clinical challenge, causing significant morbidity and healthcare costs. Persistent bacterial biofilms are critical obstacle to wound healing, necessitating their effective elimination promote rapid recovery. In photodynamic antimicrobial therapy (PDAT), enhancing the interaction between photosensitizer is key achieving efficient antibiofilm effects. Here, novel dissolvable microneedle patch containing benzoxaborole ( BOB )‐functionalized designed, TPI‐BOB , for bacteria‐specific targeting localized PDAT of multidrug‐resistant biofilm infections wounds. integrates moiety selective binding pyridine‐based cationic group enhance electrostatic interactions, showing superior activity Gram‐positive bacteria, particularly MRSA . To further optimize therapeutic delivery combat biofilm‐associated infections, incorporated into microneedles, which rapidly disintegrate upon application sites. This system facilitates localized, biofilms, where it triggers action under white‐light irradiation. treatment eradicates biofilm, initiating tissue repair that reduces inflammation, promotes collagen deposition, stimulates angiogenesis, accelerating healing. work presents strategy combining with microneedle‐mediated drug delivery, offering promising approach treating wounds other biofilm‐related infections.

Язык: Английский

Процитировано

0

Hierarchically structured conductive hydrogels for electrically programmable drug delivery in a diabetic wound healing electronic patch DOI

Zhe-Wei Lin,

Yo-Lin Chen,

Wan‐Lou Lei

и другие.

Journal of Controlled Release, Год журнала: 2025, Номер unknown, С. 113760 - 113760

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Integrated 3D-Printed Hollow Microneedle Array and Lateral Flow Immunoassay for Point-of-Care Wound Healing Monitoring DOI
Amadeo Sena‐Torralba, Marc Parrilla,

Ana Hernanz-Grimalt

и другие.

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Chronic wound management requires continuous monitoring to assess healing and guide treatment. We developed a hollow microneedle array patch integrated with lateral flow immunoassay strip address the need for convenient, home-based diagnostics. This device extracts exudate directly from matrix, overcoming limitations of conventional swab sampling, which relies on surface collection. The provides minimally invasive, rapid solution phase. delivers colorimetric signal visible naked eye, facilitating straightforward interpretation by clinicians within 10 min. In clinical study involving 90 patients, matrix metalloproteinase 8 (MMP-8) was identified as critical biomarker, achieving 80.3% sensitivity in detecting proliferative A specific MMP-8 detection limit 0.7 ng/mL, lower than threshold level phase (164.7 ng/mL). 3D-printed cost-efficiency (less €0.10 per patch) height 865 ± 6 μm, allowing painless, easy sampling. Mechanical tests confirmed durability patch, while cytotoxicity assays demonstrated its biocompatibility. Prevalidation using an

Язык: Английский

Процитировано

0