Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 24, 2025
Abstract
Nanozymes
hold
great
promise
for
biosensing
applications,
yet
their
practical
implementation
is
hampered
by
limitations
in
signal
enhancement,
reusability,
and
device
integration.
Herein,
the
development
of
a
hydrophilic
shape‐memory
nanozyme
aerogel
that
addresses
these
challenges
reported.
The
platform
constructed
embedding
chitosan‐protected
platinum
nanoparticles
(Pt‐CS
NPs),
which
exhibit
intrinsic
peroxidase
(POD)
activity,
within
polyacrylic
acid
(PAA)
matrix.
This
hybrid
Pt‐CS/PAA
retains
effect
PAA
component
as
well
POD
activity
Pt‐CS
NPs.
Importantly,
exhibits
differential
adsorption
behaviors
toward
two
distinct
oxidation
products,
oxidized
3,3′,5,5′‐tetramethyl‐benzidine
(oxTMB)
2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic
acid)
(oxABTS),
driven
differing
electrostatic
potentials
with
aerogel.
By
integrating
effect,
catalysis,
exceptional
probe
performance,
enables
realization
signal‐amplified
or
reusable
nanozyme‐based
assay,
when
employing
TMB
ABTS
chromogenic
substrates,
respectively.
Furthermore,
architecture
allows
straightforward
deviceization,
transforming
analysis
from
conventional
test
paper
formats
into
practical,
sensing
devices.
work
establishes
generalizable
blueprint
engineering
smart
materials
shape
memory
characteristics,
opening
up
new
avenues
developing
advanced
platforms
enhanced
performance
user‐friendly
operability.
The
importance
of
nanotechnology
is
increasing
every
day
in
different
fields
and,
especially,
the
application
nanomaterials
has
attracted
considerable
attention
food
safety.
Among
nanomaterials,
MXenes,
which
are
two-dimensional
(2D)
transition
metal-based
layered
materials
made
nitrides
and
carbides,
have
revolutionized
various
as
a
cutting-edge
scientific
discovery
nanotechnology.
These
been
widely
used
structure
biosensors
sensors
due
to
their
excellent
metallic
conductivity,
mechanical
stability,
optical
absorbance,
good
redox
capability,
higher
heterogeneous
electron
transfer
rate.
In
particular,
MXenes
nanozymes
highlighted
high
performance
great
extent
biosensor
domains.
growing
interest
these
attributed
specific
physicochemical
features.
key
enzymatic
features
include
activities
similar
oxidase,
peroxidase,
catalase,
superoxide
dismutase.
this
review,
initially,
several
common
synthesis
methods
presented,
emphasizing
significant
role
constructing
efficient
sensors.
Subsequently,
applications
MXene
safety
analysis
delved
into,
including
detection
bacteria,
mycotoxins,
antibiotic
residues,
pesticide
along
with
electrochemical
biosensors.
addition,
gap,
limitation,
future
perspective
novel
highlighted.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 3, 2025
Nanozymes,
while
promising
alternatives
to
natural
peroxidases
in
colorimetric
assays,
are
often
hindered
by
lower
catalytic
efficiencies.
Although
numerous
approaches
have
been
developed
improve
signal
intensity
nanozyme-based
optimization
of
the
reaction
medium
which
nanozyme
interacts
with
substrate
remains
a
significantly
underexplored
area.
The
vast
majority
studies
rely
on
standard
sodium
acetate
buffers
or
commercially
sourced
reagents
optimized
for
horseradish
peroxidase,
neglecting
unique
properties
different
nanozymes.
This
work
presents
systematic
3,3',5,5'-tetramethylbenzidine
(TMB)-based
composition
four
common
nanozymes:
iron
oxide,
LaNiO3,
Mn-doped
CeO2,
and
platinum
nanoparticles.
Our
findings
reveal
that
buffer
is
suitable
alternative
enhance
(41-68%)
other
Further
ionic
strength,
organic
cosolvent
type
concentration,
TMB/H2O2
concentrations
yielded
improvements
intensity,
analytical
sensitivity,
assay
time.
study
also
identifies
pitfalls
encountered
during
conditions
proposes
potential
solutions.
We
posit
should
be
step
development
use
undisclosed
compositions
avoided.
Nanomaterials,
Год журнала:
2024,
Номер
14(8), С. 706 - 706
Опубликована: Апрель 18, 2024
Nitrite
monitoring
serves
as
a
fundamental
practice
for
protecting
public
health,
preserving
environmental
quality,
ensuring
food
safety,
maintaining
industrial
safety
standards,
and
optimizing
agricultural
practices.
Although
many
nitrite
sensing
methods
have
been
recently
developed,
the
quantification
of
remains
challenging
due
to
sensitivity
selectivity
limitations.
In
this
context,
we
present
fabrication
enzymeless
iron
oxide
nanoparticle-modified
zinc
nanorod
(α-Fe
Nanozymes,
while
promising
alternatives
to
natural
peroxidases
in
colorimetric
assays,
are
often
hindered
by
lower
catalytic
efficiencies.
While
various
strategies
exist
enhance
signal
intensity
nanozyme-based
substrate
optimization
remains
largely
underexplored.
The
vast
majority
of
studies
rely
on
standard
sodium
acetate
buffers
or
commercially-sourced
substrates
optimized
for
horseradish
peroxidase,
neglecting
the
unique
properties
different
nanozymes.
This
work
presents
a
systematic
3,3',5,5'-tetramethylbenzidine
(TMB)-based
compositions
four
common
nanozymes:
iron
oxide,
LaNiO3,
Mn-doped
CeO2,
and
platinum
nanoparticles.
Our
findings
reveal
that
buffer
is
suitable
alternative
significantly
(41-68%)
other
Further
ionic
strength,
organic
co-solvent
type
concentration,
TMB/H2O2
concentrations
yielded
improvements
intensity,
analytical
sensitivity,
assay
time.
study
also
identifies
pitfalls
encountered
during
proposes
potential
solutions.
We
posit
composition
should
be
step
development
use
with
undisclosed
avoided.
