Spatioselective Imaging of Noncoding RNAs in Mitochondria via an Organelle-Specific DNA Assembly Strategy DOI
Bo Wu, Jian Zhao, Fangzhi Yu

и другие.

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Precise imaging of noncoding RNAs (ncRNAs) in specific organelles allows decoding their functions at subcellular level but lacks advanced tools. Here we present a DNA-based nanobiotechnology for spatially selective ncRNA (e.g., microRNA (miRNA)) mitochondria via an organelle-specific DNA assembly strategy. The target miRNA-initiated hairpins is inhibited by the block toehold-mediated strand displacement reaction can be exclusively activated mitochondria-encoded ribosomal RNA (rRNA) hybridization chain reaction, enabling spatial control over miRNA imaging. We demonstrate that conditionally controlled technology minimization nonspecific activation and thus improves precision detection. In addition, strategy adaptable to visualizing other ncRNAs such as long mitochondria, highlighting universality approach. Overall, this work provides useful tool investigating organelle-located RNA.

Язык: Английский

Spatioselective Imaging of Noncoding RNAs in Mitochondria via an Organelle-Specific DNA Assembly Strategy DOI
Bo Wu, Jian Zhao, Fangzhi Yu

и другие.

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Precise imaging of noncoding RNAs (ncRNAs) in specific organelles allows decoding their functions at subcellular level but lacks advanced tools. Here we present a DNA-based nanobiotechnology for spatially selective ncRNA (e.g., microRNA (miRNA)) mitochondria via an organelle-specific DNA assembly strategy. The target miRNA-initiated hairpins is inhibited by the block toehold-mediated strand displacement reaction can be exclusively activated mitochondria-encoded ribosomal RNA (rRNA) hybridization chain reaction, enabling spatial control over miRNA imaging. We demonstrate that conditionally controlled technology minimization nonspecific activation and thus improves precision detection. In addition, strategy adaptable to visualizing other ncRNAs such as long mitochondria, highlighting universality approach. Overall, this work provides useful tool investigating organelle-located RNA.

Язык: Английский

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