
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Авг. 23, 2024
Язык: Английский
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Авг. 23, 2024
Язык: Английский
Green Chemistry, Год журнала: 2024, Номер 26(11), С. 6480 - 6489
Опубликована: Янв. 1, 2024
A comprehensive two-step supercritical extraction using green solvents allows the of non-polar and polar analytes within one run in two consecutive steps.
Язык: Английский
Процитировано
8Separations, Год журнала: 2024, Номер 11(8), С. 233 - 233
Опубликована: Июль 29, 2024
Peptides are gaining prominence in various fields, including the pharmaceutical industry. To meet regulatory requirements, they must achieve a certain purity threshold to ensure safe administration. Numerous purification technologies have been employed purify peptides, aiming reduce cost and time while being sustainable efficient. These include chromatography, magnetic nanoparticles, isoelectric focusing, membrane filtration. The physicochemical properties of peptides main driving element behind these technologies. While chromatographic separation remains gold standard for peptide purification, with models predict elution behaviors other demonstrated their capability performance established methodologies, better productivity reduced cost. This opens door further investigational studies assess outcomes potentially introduce new techniques purification. In this review, we examine terms efficiency ability sustainability concluding remarks an outlook on future advancements.
Язык: Английский
Процитировано
7Chemometrics and Intelligent Laboratory Systems, Год журнала: 2025, Номер unknown, С. 105435 - 105435
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Ноя. 5, 2024
In this study, a direct HPLC method was developed to determine the enantiomeric purity of immunomodulatory drug, ozanimod. A systematic development process followed, incorporating risk assessment, identification critical analytical procedure parameters, initial screening stationary phases, and software-assisted optimization parameters. Eight different polysaccharide-based chiral columns were selected assess separation enantiomers under polar organic elution mode. The most promising results obtained using methanol:2-propanol mixture on amylose-based Chiralpak AD column. Following this, modeling conducted DryLab software optimize conditions, including isocratic eluent composition, temperature, flow rate. Baseline achieved within fifteen minutes optimized parameters: column thermostated at 10 °C, mobile phase methanol:2-propanol: diethylamine, 70:30:0.1 (v/v/v %), delivered rate 0.8 mL/min. validated according current guidelines in silico robustness testing tolerance limits for parameters their impact enantioresolution. Our findings demonstrate utility DryLab, typically employed reversed-phase achiral separations, optimizing methods even Limitations approach are also highlighted.
Язык: Английский
Процитировано
1Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Авг. 23, 2024
Язык: Английский
Процитировано
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