Separation Science Plus, Год журнала: 2025, Номер 8(5)

Опубликована: Май 1, 2025

ABSTRACT To reveal the chemical composition of Cnidium officinale Makino (COM), improve quality control standard, and explore related toxicity, solvent extraction, SiO 2 gel column chromatography, nuclear magnetic resonance (NMR, 1 H& 13 C) technology were used to extract, isolate, identify structures secondary metabolites from COM. A total 10 batches COM different origins studied by HPLC; data HPLC analyzed chemometry. At same time, using zebrafish as an experimental animal model, effects polar parts on embryo development explored. The five compounds identified in experiment, namely senkyunolide A, H, ligustrolide I, ferulic acid, chlorogenic acid. fingerprint was established, 19 common peaks identified, seven which characteristic peaks. Toxicity studies showed that fractions ( n ‐hexane, chloroform, ethyl acetate, ‐butanol, water fractions) did not show significant toxicity when dose concentration between 0.5 1.5 mg/mL, but up 2.5 mg/mL. Zebrafish embryos degrees spinal curvature, pericardial edema, delayed yolk sac absorption, developmental retardation. This research has preliminarily elucidated foundation It successfully established fingerprint‐based standard for put forward safe application ranges extracts diverse fractions. These achievements have furnished subsequent in‐depth investigations.

Язык: Английский