Abstract
In
pharmaceuticals,
the
structural
and
functional
alterations
induced
by
biotransformation
are
well‐documented.
Many
pharmaceuticals
exist
in
various
crystal
forms,
which
govern
their
transformation
significantly
impact
activity.
However,
field
of
inorganic
nanomedicine,
there
is
a
paucity
research
focusing
on
influence
form‐dependent
“metabolism”
(transformation)
activity
biomechanism.
This
study
delves
into
distinct
performances
two
forms
manganese
sulfide
(MnS),
namely
α‐MnS
γ‐MnS,
bacteria‐infected
diabetic
wound
healing.
initial
stage
wound,
where
environment
neutral
to
slightly
alkaline,
MnS
partially
converts
Mn
x
O
y
(comprising
2
3
4
)
concurrently
produces
hydrogen
(H
S);
conversion
efficiency
γ‐MnS
surpasses
that
α‐MnS.
Additionally,
more
soluble
than
α‐MnS,
allows
it
generate
2+
.
These
components
collectively
contribute
superior
bacteriostatic
properties
MnS.
related
cells,
stimulates
production
collagen
I
vascular
endothelial
growth
factor
(VEGF),
promote
M1
macrophages
polarizing
M2
phenotype,
for
extracellular
matrix
(ECM)
remodeling.
Notably,
different
products
have
functions.
Consequently,
dependent
its
original
form
solubility
efficiency.
International Journal of Pharmaceutics X,
Год журнала:
2025,
Номер
unknown, С. 100328 - 100328
Опубликована: Март 1, 2025
Infected
wounds
are
common
clinical
injuries
that
often
complicated
by
inflammation
and
oxidative
stress
due
to
bacterial
invasion.
These
typically
suffer
from
impaired
vascularization,
which
delays
healing
increases
the
risk
of
complications
such
as
sepsis
chronic
wounds.
Therefore,
developing
an
effective
treatment
for
infected
is
highly
necessary.
Egg
white
can
promote
cell
regeneration
repair,
while
chitosan
in
resisting
Sildenafil
believed
have
potential
angiogenesis.
Based
on
these
properties,
we
prepared
a
new
type
hydrogel
using
egg
framework,
loaded
with
sildenafil
(CEHS).
The
combines
benefits
its
components,
exhibiting
good
biocompatibility
promoting
proliferation
migration
NIH
3T3
(3T3)
cells
human
umbilical
vein
endothelial
(HUVEC),
well
angiogenesis
HUVEC.
It
also
exhibits
significant
antioxidant,
anti-inflammatory,
antibacterial
properties
against
Escherichia
coli
(E.
coli)
Staphylococcus
aureus
(S.
aureus).
Additionally,
mouse
model
wounds,
CEHS
effectively
promoted
wound
through
excellent
antioxidant
anti-inflammatory
activity,
pro-angiogenic
effects.
In
summary,
this
simple-to-prepare,
multifunctional
natural
shows
great
promise
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 24, 2025
Cationic
antimicrobial
agents
are
widely
recognized
for
combating
microbial
infections
through
their
membrane-disruptive
properties.
Recently,
sulfur
dioxide
(SO2)
gas
therapy
has
emerged
as
a
promising
alternative
treating
diseases,
including
bacterial
infections.
However,
current
systems
often
target
only
specific
strains.
Herein,
we
present
amphiphilic
alternating
copolymers,
DAPx
(x
=
1,
2,
3),
incorporating
cationic
residues
and
thiol-responsive
SO2-releasing
moieties.
In
aqueous
environments,
copolymers
self-assemble
into
micellar
nanoassemblies
(DAPxNp),
exposing
hydrophilic
outward
encapsulating
hydrophobic
moieties
within
the
core
to
enable
controlled
sustained
release
of
SO2
in
presence
glutathione
(GSH).
vitro
studies
reveal
excellent
biocompatibility
DAP2
Np
with
broad-spectrum
antibacterial
activity
against
both
Gram-positive
(Bacillus
subtilis,
Staphylococcus
aureus)
Gram-negative
(Escherichia
coli,
Pseudomonas
aeruginosa)
bacteria.
Mechanistic
investigations
confirm
eradication
via
membrane
disruption
reactive
oxygen
species
generation.
This
study
underscores
remarkable
efficacy
polymers
resisting
CytoJournal,
Год журнала:
2025,
Номер
22, С. 46 - 46
Опубликована: Апрель 25, 2025
Burns
refers
to
a
severe
form
of
trauma
that
often
leads
localized
and
systemic
inflammatory
responses,
oxidative
stress,
immune
dysfunction.
Patients
with
burns
are
highly
susceptible
the
development
postburn
sepsis,
condition
influenced
by
multiple
factors,
such
as
bacterial
infection
burn
wound,
alterations
in
status,
excessive
release
mediators.
This
study
aimed
investigate
mechanisms
which
hydrogen
gas
treatment
exerts
its
effects
on
focus
influence
wound
healing.
work
employed
vitro
assays
Sprague-Dawley
(SD)
rat
skin
fibroblasts
(RSFs)
assess
serum
cell
proliferation
through
methylthiazolyldiphenyltetrazolium
bromide
apoptosis
flow
cytometry
Annexin
V-fluorescein
isothiocyanate/propidium
iodide
staining.
In
addition,
an
enzyme-linked
immunosorbent
assay
was
performed
quantify
cytokines
stress
markers
treated
serum.
Western
blotting
(WB)
analysis
conducted
signaling
pathway
modulation.
The
sepsis
models
SD
rats
were
segregated
into
three
experimental
groups:
healthy
normal
control
group,
+
(2%)
group.
Wound
healing
monitored,
contraction
rates
recorded
histological
assessments
using
hematoxylin
eosin
Masson's
trichrome
staining
evaluate
tissue
repair
collagen
deposition.
showed
reduced
fibroblast
increased
(P
<
0.01),
mitigated
rescuing
viability
reducing
0.01).
Enzyme-linked
revealed
serum-induced
increases
levels
markers,
decreases
antioxidant
enzymes
reversed
0.05).
WB
suggested
gas's
anti-inflammatory
proliferative
modulating
pathways
vivo,
considerably
improved
healing,
accelerated
enhanced
Plasma
analyses
indicated
local
from
gas.
Hydrogen
demonstrates
potential
therapeutic
efficacy
management
promoting
These
findings
provide
scientific
evidence
supporting
adjunctive
strategy
for
sepsis.
Gels,
Год журнала:
2025,
Номер
11(5), С. 354 - 354
Опубликована: Май 12, 2025
Nitric
oxide
(NO)
is
an
endogenous
signaling
molecule
that
plays
a
critical
role
in
wound
healing.
However,
the
gaseous
nature,
short
half-life,
and
low
stability
of
NO
present
challenges
for
its
clinical
application.
To
address
these
issues,
this
study
introduces
innovative
S-nitrosoglutathione
(GSNO)-loaded
asymmetric
alginate
(SA)
hydrogel
(GSNO-SA)
as
novel
solution
treating
infected
chronic
wounds.
The
designed
with
layer-by-layer
melting-permeation
crosslinking
approach,
forming
dense
upper
layer
sparse
lower
structure,
effectively
promoting
exudate
management
while
delaying
release.
results
demonstrate
GSNO-SA
extends
release
up
to
48
h,
exhibits
rapid
absorption
(72.3
±
1.5%
equilibrium
swelling
after
5
min),
significant
antibacterial
activity
(over
90%
rate
against
E.
coli
S.
aureus),
anti-inflammatory
effects
(marked
reduction
TNF-α
expression),
promotes
angiogenesis
(90.00
5.92%
migration
at
h).
Additionally,
animal
studies
show
accelerates
healing,
achieving
99.2
0.1%
closure
14
days.
Histological
immunohistochemical
evaluations
further
confirm
ability
regulate
inflammation
(13.34-fold
upregulation
CD163)
promote
(3.02-fold
α-SMA).
Theoretically,
design
provides
strategy
developing
exudate-managing
dressings
by
integrating
controlled
hierarchical
pore
structures.
