Trematode Species Detection and Quantification by Environmental DNA-QPCR Assay in Lake Chany, Russia DOI

J. Gacad Janelle Laura,

Natalia I. Yurlova,

Shoko Tanabe-Hosoi

и другие.

Journal of Parasitology, Год журнала: 2024, Номер 110(3)

Опубликована: Июнь 19, 2024

Environmental DNA (eDNA) surveys promise to be a sensitive and powerful tool for the detection of trematodes. This can contribute limited studies on trematode ecology, specifically in aquatic ecosystems. Here, we developed species-specific primer probe sets Moliniella anceps, Opisthioglyphe ranae, Plagiorchis multiglandularis cercariae applied novel eDNA qPCR assay detect larval trematodes quantitatively. We evaluated effectiveness assays using filtered lake water samples collected from different sites Lake Fadikha Kargat River Estuary Chany, Russia, showing high species specificity sensitivity all 3 assays. Further, had efficiencies ranging 94.9 105.8%. O. P. were detected environmental through real-time PCR. Thus, anticipate that our approach will beneficial biomonitoring, measuring, managing ecological systems.

Язык: Английский

Community ecology in a bottle: Leveraging eDNA metabarcoding data to predict occupancy of co‐occurring species DOI Creative Commons
Sasha J. Tetzlaff, Aron D. Katz, Mark D. Johnson

и другие.

Environmental DNA, Год журнала: 2024, Номер 6(3)

Опубликована: Май 1, 2024

Abstract Detecting environmental DNA (eDNA) of numerous organisms from the same samples has been revolutionized by metabarcoding. However, utilizing vast amounts data generated metabarcoding to predict occupancy probabilities for co‐occurring species is currently rare. Here, we demonstrate how can be used advance community ecology research through a case study using replicate stream water and Bayesian models test hypotheses eDNA occurrence native fish (brook trout, Salvelinus fontinalis ), its major ectoparasite (gill lice, Salmincola edwardsii an introduced potential competitor (brown Salmo trutta ). Gill lice was positively associated with brook trout biomass determined via electrofishing conducted near sampling sites, suggesting gill dependent on host density. Leveraging site‐specific molecular operational taxonomic units identified metabarcoding, often predicted richness aquatic insect orders commonly feed on, which are also quality indicators. Thus, high‐quality habitats that environmentally sensitive salmonids their primary prey rely may promote higher rates, further facilitating spread parasites. An increasing amount community‐level being global efforts, suggest our framework could broadly implemented enhance understanding factors impacting distributions species, reveal new ecological phenomena, support management conservation efforts.

Язык: Английский

Процитировано

4
The Use of Environmental DNA as Preliminary Description of Invertebrate Diversity in Three Sicilian Lakes DOI Creative Commons
Manuela Mauro, Francesco Longo, Mario Lo Valvo

и другие.

Animals, Год журнала: 2025, Номер 15(3), С. 355 - 355

Опубликована: Янв. 26, 2025

Freshwater ecosystems are among the most severely affected environments by species loss caused climate change and intense anthropogenic pressure. To preserve biodiversity, biomonitoring plays a key role providing reliable data on biological diversity ecological status. Environmental DNA (eDNA) metabarcoding has emerged as powerful non-invasive alternative to traditional morphology-based sampling identification methods. This study represents first application of eDNA analysis assess invertebrate communities in three Sicilian Lakes: Poma, Piana degli Albanesi Scanzano. Water samples were collected at two points each lake after filtration with nitrocellulose membranes, was extracted performed. A total 27 identified, belonging Phyla Annelida, Arthropoda Rotifera. Notably, revealed presence alien (Daphnia parvula Acanthocyclops americanus), dangerous associated transmission viral diseases (Culex pipiens), potential new records for Sicily (Stylaria lacustris, Platypalpus exilis, Pammene aurana, Limnephilus rhombicus). These results provide preliminary snapshot biodiversity these sites, demonstrating how complement, but not replace, methods, contributing assessment ecosystem

Язык: Английский

Процитировано

0
Environmental conditions influencing the abundance of the salmonid ectoparasite Salmincola californiensis across upper Willamette River Reservoirs, Oregon DOI Creative Commons

Kelsi Antonelli,

C. Murphy, Amanda M. Pollock

и другие.

PeerJ, Год журнала: 2025, Номер 13, С. e19228 - e19228

Опубликована: Апрель 7, 2025

The freshwater copepod Salmincola californiensis is an ectoparasite of Pacific salmon and trout ( Oncorhynchus spp.). High levels infection by this parasite can significantly damage gills result in blood loss, affecting the fitness survival hosts, it may hinder recovery efforts threatened endangered salmonids. Juvenile salmonids that rear reservoirs have been reported to experience higher rates than their stream-dwelling counterparts. To date, relationship between reservoir environmental conditions remains poorly understood. Here, we evaluated sampling methods designed capture S. copepodids, free-swimming infectious life stage parasitic copepod, develop predictive models abundance . We used light traps collect 675 zooplankton samples from various sites depths Cougar, Lookout Point, Fall Creek reservoirs, Oregon, USA during five months 2020. tested several using covariates obtained concurrently plankton surveys e.g. , temperature, ambient water clarity, profiles, flow). Our showed a strong influence temperature on with maximum initial occurring at mean 15.2 °C. At August was estimated be 58.6 copepodids per site Cougar Reservoir, 43.8 3.5 Point Reservoir. Water outflow both affected population growth copepodids: increased associated increase rate while decrease rate. efficacy our method influenced percent moon fullness, whether sample above or below thermocline, number juvenile sculpin fishes Cottus spp.) captured trap. probability detecting single copepodid if one present 0.042, but detection 0.29 under ideal trap set warmer water, location full moon. Biologists managers could use these assess presence other locations, inform operations reduce potential salmonid infections.

Язык: Английский

Процитировано

0
Comparison of qPCR and metabarcoding for environmental DNA surveillance of a freshwater parasite DOI Creative Commons
Mark D. Johnson, Sasha J. Tetzlaff, Aron D. Katz

и другие.

Ecology and Evolution, Год журнала: 2024, Номер 14(5)

Опубликована: Май 1, 2024

Abstract Analysis of environmental DNA (eDNA) has been successfully used across freshwater ecological parasitology to inform management ecologically and economically important species. However, most studies have species‐specific quantitative polymerase chain reaction (qPCR) assays detect target taxa. While generally effective, this approach limits the amount community management‐supporting data that can be obtained from eDNA samples. If metabarcoding could conducted with same accuracy a single species approach, researchers simultaneously while obtaining vast We sampled 38 sites on Fort McCoy, Wisconsin compared qPCR for detection ectoparasitic gill louse Salmincola edwardsii , an obligate parasite Salvelinus fishes (chars). found no evidence suggest S. occupancy or probabilities differed between metabarcoding. Further, we number reads were negatively predictive C T values ( value indicates cycle significant is detected, lower s indicative more DNA), demonstrating our positively predicted quantities. was not overall score (number positive replicates). In addition led over 2600 invertebrate underscore necessity conducting similar analyses environments species, as ecology will vary per‐study basis. Our results provides highly sensitive accurate method detecting parasitic lice also illuminating broader biological co‐occurrence in environment.

Язык: Английский

Процитировано

3
Comparison of soil eDNA to camera traps for assessing mammal and bird community composition and site use DOI Creative Commons
Sasha J. Tetzlaff, Aron D. Katz, P. Wolff

и другие.

Ecology and Evolution, Год журнала: 2024, Номер 14(7)

Опубликована: Июль 1, 2024

Species detections often vary depending on the survey methods employed. Some species may go undetected when using only one approach in community-level inventory and monitoring programs, which has management conservation implications. We conducted a comparative study of terrestrial mammal bird spring summer 2021 by placing camera traps at 30 locations across large military installation northern Michigan, USA testing replicate soil samples from these sites for environmental DNA (eDNA) an established vertebrate metabarcoding assay. detected total 48 taxa both methods: 26 mammalian (excluding humans, 24 to two genus) 22 avian (21 genus). relatively even distribution cameras (17) via eDNA analysis (15), with seven methods. Most medium-to-large carnivores were cameras, whereas semi-fossorial small mammals analysis. higher diversity (18 taxa) compared (eight taxa; four methods), but alone most effective detecting smaller birds that frequently occupy arboreal environments. also used Bayesian spatial occupancy models widely distributed game (white-tailed deer,

Язык: Английский

Процитировано

3
Fish ectoparasite detection, collection and curation DOI
Xian Zhe Chew, JM Cobcroft, Kate S. Hutson

и другие.

Advances in Parasitology/Advances in parasitology, Год журнала: 2024, Номер unknown, С. 105 - 157

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

2
Environmental DNA sampling for at-risk and invasive species management on military ranges : guidelines and protocols for installation biologists and land managers DOI Open Access
Aron D. Katz, Mark D. Johnson, Jinelle H. Sperry

и другие.

Опубликована: Март 5, 2024

Environmental DNA (eDNA) analysis, or the detection of trace shed by organisms into their environment, has potential to transform Army capabilities for threatened and endangered species (TES) invasive management providing a rapid, noninvasive, cost-effective option monitoring wildlife. Despite these benefits, eDNA analysis is underutilized on military installations as limited access guidance materials, protocols, training opportunities, support from scientists makes it difficult installation biologists land managers design execute surveys, let alone identify questions that may benefit monitoring. Therefore, aim this resource increase awareness benefits limitations provide study guidelines field sampling protocols nonexperts make tool more accessible help facilitate adoption eDNA-based approaches wildlife ranges.

Язык: Английский

Процитировано

0
Trematode Species Detection and Quantification by Environmental DNA-QPCR Assay in Lake Chany, Russia DOI

J. Gacad Janelle Laura,

Natalia I. Yurlova,

Shoko Tanabe-Hosoi

и другие.

Journal of Parasitology, Год журнала: 2024, Номер 110(3)

Опубликована: Июнь 19, 2024

Environmental DNA (eDNA) surveys promise to be a sensitive and powerful tool for the detection of trematodes. This can contribute limited studies on trematode ecology, specifically in aquatic ecosystems. Here, we developed species-specific primer probe sets Moliniella anceps, Opisthioglyphe ranae, Plagiorchis multiglandularis cercariae applied novel eDNA qPCR assay detect larval trematodes quantitatively. We evaluated effectiveness assays using filtered lake water samples collected from different sites Lake Fadikha Kargat River Estuary Chany, Russia, showing high species specificity sensitivity all 3 assays. Further, had efficiencies ranging 94.9 105.8%. O. P. were detected environmental through real-time PCR. Thus, anticipate that our approach will beneficial biomonitoring, measuring, managing ecological systems.

Язык: Английский

Процитировано

0