Life,
Год журнала:
2023,
Номер
13(12), С. 2254 - 2254
Опубликована: Ноя. 25, 2023
The
aim
of
the
current
work
was
to
examine
for
first
time
nephropreventive
capacity
Ephedra
alata
seed
extract
(E)
against
maternal
exposure
acephate
in
rat
offspring.
vivo
results
revealed
that
E.
supplementation
28
days
(40
mg/kg
b.w.)
significantly
attenuated
nephrotoxicity
adult
offspring
induced
by
acephate.
In
fact,
it
decreased
levels
creatinine
and
uric
acid
increased
albumin
content
compared
intoxicated
group.
utero
studies
showed
inhibited
renal
oxidative
stress
generated
reducing
lipid
peroxidation
enhancing
antioxidant
biomarker
activities
(GSH,
CAT,
SOD).
inhibition
DNA
fragmentation
improvement
ultrastructural
changes
highlighted
prophylactic
effect
tissue.
Additionally,
immunofluorescence
study
upregulation
LC3
gene
expression,
suggesting
stimulate
autophagic
processes
as
a
protective
mechanism.
Molecular
docking
analysis
indicated
hexadecasphinganine,
major
compound
alata,
has
higher
affinity
toward
Na+/K+-ATPase,
epithelial
sodium
channel
(ENaC),
hydrogen
exchanger
3
(NHE3)
genes
than
Hexadecasphinganine
could
be
considered
potential
inhibitor
activity
these
therefore
exerted
its
preventive
capacity.
obtained
findings
confirmed
capacities,
which
related
bioactive
compounds,
possess
activities.
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi,
Год журнала:
2024,
Номер
27(2), С. 304 - 315
Опубликована: Янв. 18, 2024
The
adverse
effects
of
tetramethrin
residues,
a
synthetic
pyrethroid
used
in
many
insecticide
formulations,
on
environmental
health
and
living
organisms
are
matter
concern.
aim
this
study
was
to
evaluate
the
genotoxic
developmental
non-target
organism,
Drosophila
melanogaster.
Thus,
its
effect
DNA
damage
evaluated
using
Comet
assay
hemocytes,
mutagenic
recombinogenic
were
wing
SMART.
Also,
development
by
measuring
larval
weight,
length,
fecundity.
Results
showed
that
induced
decrease
weight
length
only
at
high
concentration.
Moreover,
fecundity
dose-dependent
manner
observed.
According
results,
not
because
there
no
significant
increase
%
DNA.
However,
caused
genotoxicity
inducing
mitotic
recombination
SMART
assay.
Environmental Toxicology,
Год журнала:
2023,
Номер
38(9), С. 2182 - 2191
Опубликована: Июль 12, 2023
Abstract
Background
Septic
acute
kidney
injury
(SAKI)
is
usually
caused
by
sepsis.
It
has
been
shown
that
catalpol
(Cat)
impairs
sepsis‐evoked
organ
dysfunction
to
a
certain
degree.
The
current
work
aims
evaluate
the
protective
effects
of
Cat
on
SAKI
and
potential
mechanisms
in
vivo
vitro.
Methods
cellular
murine
model
were
set
up
using
lipopolysaccharide
(LPS)
vitro
vivo.
Cell
apoptosis
cells
was
determined
TUNEL
assay.
Levels
inflammatory
cytokines
detected
enzyme‐linked
immunosorbent
assay
(ELISA).
levels
markers
oxidative
evaluated
corresponding
commercial
kits.
Protein
assayed
via
western
blotting
immunohistochemistry
(IHC)
staining.
Results
results
demonstrated
LPS
upregulated
TNF‐α,
IL‐6,
malondialdehyde
levels,
downregulated
superoxide
dismutase,
whereas
treated
have
opposite
results.
Functional
assays
displayed
remarkably
reversed
LPS‐challenged
damage
as
impairment
TNF‐α
IL‐6
stress,
HK‐2
cells.
Moreover,
knockdown
Sirtuin
1
(Sirt1)
counteracted
suppressive
impact
LPS‐triggered
response,
renal
damage.
Further,
elevated
Sirt1
expression
activated
Nrf2/HO‐1
signaling
LPS‐engendered
Conclusion
Our
study
clearly
proved
protected
against
LPS‐induced
synergic
antioxidant
anti‐inflammatory
actions
regulating
pathways.
Medicine,
Год журнала:
2024,
Номер
103(5), С. e37089 - e37089
Опубликована: Фев. 2, 2024
Gouty
arthritis
(GA)
is
an
inflammatory
disease
caused
by
disorders
of
the
purine
metabolism.
Although
increasing
number
drugs
have
been
used
to
treat
GA
with
deepening
relevant
research,
still
cannot
be
cured
simple
drug
therapy.
The
nuclear
factor-kappa
B
(NF-κB)
signaling
pathway
plays
a
key
role
in
pathogenesis
GA.
A
considerable
Chinese
herbal
medicines
emerged
as
new
for
treatment
This
article
collected
research
on
traditional
medicine
monomers
using
NF-κB,
GA,
etc.
keywords;
and
conducted
systematic
search
published
articles
PubMed
database.
In
this
study,
we
analyzed
therapeutic
effects
existing
literature
through
vivo
vitro
experiments
animal
cell
models.
Based
review,
believe
that
can
NF-κB
are
potential
development
targets.
study
provides
ideas
application
Life,
Год журнала:
2023,
Номер
13(12), С. 2254 - 2254
Опубликована: Ноя. 25, 2023
The
aim
of
the
current
work
was
to
examine
for
first
time
nephropreventive
capacity
Ephedra
alata
seed
extract
(E)
against
maternal
exposure
acephate
in
rat
offspring.
vivo
results
revealed
that
E.
supplementation
28
days
(40
mg/kg
b.w.)
significantly
attenuated
nephrotoxicity
adult
offspring
induced
by
acephate.
In
fact,
it
decreased
levels
creatinine
and
uric
acid
increased
albumin
content
compared
intoxicated
group.
utero
studies
showed
inhibited
renal
oxidative
stress
generated
reducing
lipid
peroxidation
enhancing
antioxidant
biomarker
activities
(GSH,
CAT,
SOD).
inhibition
DNA
fragmentation
improvement
ultrastructural
changes
highlighted
prophylactic
effect
tissue.
Additionally,
immunofluorescence
study
upregulation
LC3
gene
expression,
suggesting
stimulate
autophagic
processes
as
a
protective
mechanism.
Molecular
docking
analysis
indicated
hexadecasphinganine,
major
compound
alata,
has
higher
affinity
toward
Na+/K+-ATPase,
epithelial
sodium
channel
(ENaC),
hydrogen
exchanger
3
(NHE3)
genes
than
Hexadecasphinganine
could
be
considered
potential
inhibitor
activity
these
therefore
exerted
its
preventive
capacity.
obtained
findings
confirmed
capacities,
which
related
bioactive
compounds,
possess
activities.
