Cytological changes in radiation-induced lung injury

Life Sciences, Год журнала: 2024, Номер unknown, С. 123188 - 123188

Опубликована: Окт. 1, 2024

Язык: Английский

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Cadmium exposure induced spleen inflammation by activating the MAPK/NF-κB/ NLRP3 signaling pathway and the intervention effect of Astilbin

Veterinary Immunology and Immunopathology, Год журнала: 2025, Номер 281, С. 110889 - 110889

Опубликована: Янв. 31, 2025

Язык: Английский

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Astilbin Alleviates Radiation-Induced Pulmonary Fibrosis via circPRKCE Targeting the TGF-β/Smad7 Pathway to Inhibit Epithelial–Mesenchymal Transition

Biomedicines, Год журнала: 2025, Номер 13(3), С. 689 - 689

Опубликована: Март 11, 2025

Purpose: This study aimed to clarify the protective effect of astilbin (AST) on radiation-induced pulmonary fibrosis (RIPF) and explore its underlying molecular mechanism, focusing non-coding RNAs. Methods: Mouse lung epithelial cells (MLE-12 TC-1) C57BL/6J mice were used establish in vitro radiation injury models vivo RIPF models, respectively. Cell viability, apoptosis, epithelial-to-mesenchymal transition (EMT), fibrosis-related markers assessed using cell-counting kit-8 assays, Western blotting, immunohistochemistry, histological staining. High-throughput sequencing identified differentially expressed circRNAs. The mechanistic studies included RNA-FISH, a dual-luciferase reporter assay, an RNA immunoprecipitation (RIP) loss-of-function experiments. Results: AST significantly alleviated apoptosis EMT vitro, as well vivo. treatment reduced collagen deposition, protein expression, marker changes. revealed that upregulated circPRKCE, functions through ceRNA mechanism by binding miR-15b-5p, thereby promoting Smad7 expression suppressing TGF-β/Smad7 pathway. Knockdown circPRKCE abolished AST’s effects, confirming pivotal role mediating anti-fibrotic activity. Conclusions: demonstrates Astilbin alleviates via targeting pathway inhibit EMT, suggesting potential therapeutic agent for managing this severe complication radiotherapy.

Язык: Английский

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Astilbin Ameliorates Intracerebral Hemorrhage-induced Secondary Brain Injury by upregulating Treg Cells in mice

Biochemical and Biophysical Research Communications, Год журнала: 2025, Номер unknown, С. 151805 - 151805

Опубликована: Апрель 1, 2025

Язык: Английский

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