Fumonisin B1 induces oxidative stress, inflammation and necroptosis in IPEC-J2 cells
Veterinary Research Communications,
Год журнала:
2025,
Номер
49(3)
Опубликована: Апрель 8, 2025
Язык: Английский
Omics analysis revealed the intestinal toxicity induced by aflatoxin B1 and aflatoxin M1
Ecotoxicology and Environmental Safety,
Год журнала:
2024,
Номер
278, С. 116336 - 116336
Опубликована: Апрель 30, 2024
Aflatoxin
B1
(AFB1),
a
common
mycotoxin,
can
occur
in
agricultural
products.
As
metabolite
of
AFB1,
aflatoxin
M1
(AFM1)
mainly
exist
dairy
These
two
mycotoxins
threaten
human
health,
although
it
is
unclear
how
they
affect
the
function
intestinal
barrier.
In
this
study,
mice
were
exposed
to
AFB1
(0.3
mg/kg
body
b.w.)
and
AFM1(3.0
either
individually
or
combination
for
28
days
explore
main
differentially
expressed
proteins
(DEPs)
associated
enriched
pathways.
findings
preliminarily
verified
by
transcriptomic
proteomic
analyses
differentiated
Caco-2
cells.
The
results
revealed
that
AFM1
exposure
disrupted
barrier,
combined
toxicity
was
greater
than
each
toxin
alone.
Further
analysis
demonstrated
mechanisms
underlying
these
differences
could
be
explained
as
follows:
(i)
lipid
metabolism
AFB1-induced
DEPs.
(ii)
protein
export
pathway
stimulated
AFM1-induced
(iii)
cell
metabolic
ability
inhibited
(as
evidenced
changes
UDP-GT1,
UDP-GT2,
Gatm6),
apoptosis
induced
(MAP4K3),
epithelial
integrity
(Claudin7
IQGAP2),
resulting
more
extensive
damage
after
treatment.
conclusion,
hazardous
impact
co-exposure
from
perspectives
present
study.
Язык: Английский
The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(16), С. 8764 - 8764
Опубликована: Авг. 12, 2024
Fumonisin
B1
(FB1),
a
mycotoxin
produced
by
Fusarium
species,
is
prevalent
in
crops
and
animal
feed,
posing
significant
health
risks
to
livestock
humans.
FB1
induces
oxidative
stress
Sertoli
cells,
destroys
testicular
structure,
affects
spermatogenesis.
However,
methods
mitigate
the
reproductive
toxicity
of
testes
remain
unknown.
Quercetin,
natural
flavonoid
antioxidant,
may
offer
protective
benefits.
This
study
investigated
effects
mechanisms
quercetin
against
FB1-induced
TM4
cells
(a
cell
line).
The
results
indicated
that
40
μM
improved
viability,
reduced
apoptosis,
preserved
functions.
Quercetin
also
decreased
reactive
oxygen
species
(ROS)
levels
exposed
FB1,
enhanced
expression
antioxidant
genes,
mitochondrial
membrane
potential.
Compared
with
alone,
combination
increased
ATP
levels,
as
well
pyruvate
lactic
acid,
key
glycolysis
products.
Furthermore,
this
elevated
mRNA
protein
glycolysis-related
including
glucose-6-phosphate
isomerase
1
(Gpi1),
hexokinase
2
(Hk2),
aldolase
(Aldoa),
kinase,
muscle
(Pkm),
lactate
dehydrogenase
A
(Ldha)
phosphofructokinase,
liver,
B-type
(Pfkl).
boosted
activity
PKM
LDHA,
two
crucial
glycolytic
enzymes.
In
summary,
mitigates
reducing
ROS
enhancing
glycolysis.
offers
new
insights
into
preventing
treating
toxic
damage
male
system
highlights
potential
application
quercetin.
Язык: Английский
Preparation and Application of Clostridium perfringens Alpha Toxin Nanobodies
Veterinary Sciences,
Год журнала:
2024,
Номер
11(8), С. 381 - 381
Опубликована: Авг. 19, 2024
All
subtypes
of
Clostridium
perfringens
(C.
perfringens)
produce
the
alpha
toxin
(CPA),
which
can
cause
enteritis
or
enterotoxemia
in
lambs,
cattle,
pigs,
and
horses,
as
well
traumatic
clostridial
myonecrosis
humans
animals.
CPA
acts
on
cell
membranes,
ultimately
leading
to
endocytosis
death.
Therefore,
neutralization
is
crucial
for
prevention
treatment
diseases
caused
by
C.
perfringens.
In
this
study,
utilizing
an
antigen,
a
nanobody
(CPA-VHH)
with
half-life
2.9
h,
affinity
constant
(KD)
0.9
nmol/L,
good
stability
below
60
°C
was
prepared
from
natural
library
alpacas.
The
biological
activity
analysis
CPA-VHH
revealed
its
ability
effectively
neutralize
phospholipase
hemolytic
at
15-fold
ratio.
Vero
cells,
9.8
μg/mL
neutralized
cytotoxicity
two
times
half-maximal
inhibitory
concentration
(IC50).
mouse
model,
35.7
ng/g
body
weight
(BW)
90%
lethality
2×
median
lethal
dose
(LD50)
CPA.
It
found
that
protected
80%
mice
within
30
min
2
×
LD50
CPA,
but
dropped
50%
after
h
0%
4
h.
Rescue
trials
indicated
using
post-infection
achieved
rescue
rate,
decreased
10%
Furthermore,
mitigated
reduction
expression
levels
zonula
occludens-1
(ZO-1),
Occludin,
Claudin-1,
while
also
attenuating
upregulation
pro-inflammatory
cytokines
interleukin-1β
(IL-1β),
interleukin-6
(IL-6),
interleukin-7
(IL-7),
interleukin-8
(IL-8),
tumor
necrosis
factor
α
(TNF-α),
interferon-γ
(IFN-γ)
induced
infection.
Overall,
study
has
identified
specific
nanobody,
CPA-VHH,
neutralizes
toxins
vitro
animal
models,
providing
new
tool
inhibiting
pathogenicity
resulting
these
laying
important
foundation
development
anti-C.
toxin-related
therapeutic
products.
Язык: Английский
Formation mechanism, prevention of malignant ascites effusion and reduction of intestinal mucosal irritation of natural microemulsion from Euphorbia lathyris Pulveratum
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
178, С. 117253 - 117253
Опубликована: Авг. 7, 2024
Malignant
ascites
effusion
(MAE)
is
a
common
complication
of
advanced
malignant
tumors
with
limited
treatments.
Euphorbia
lathyris
(EL)
has
long
history
application
in
patients
edema
and
ascites.
Herein,
we
reported
for
the
first
time
mode
which
EL
Pulveratum
(PEL)
spontaneously
formed
natural
microemulsions
(ELM
PELM)
without
addition
any
carriers
excipients,
found
that
protein
phospholipid
contained
them
encapsulated
fatty
oil
diterpenoid
esters
through
non-covalent
interactions.
The
denaturation
degradation
PELM
resulted
stronger
binding
to
hydrophobic
region
protein,
facilitated
sustained
slow
release
improved
their
bioavailability
vivo,
thereby
retaining
efficacy
preventing
MAE
while
alleviating
irritation
intestinal
mucosa.
mechanism
by
retained
might
be
related
increased
feces
moisture
urine
volume,
decreased
expression
AVPR2,
cAMP,
PKA
AQP3
mice.
And
its
reducing
mucosal
was
cell
apoptosis,
amelioration
oxidative
stress,
elevation
mitochondrial
membrane
potential,
up-regulation
Occludin
Claudin-1
IEC-6
cells.
This
nano-adjuvant-free
may
promising
therapeutic
strategy
field
phytochemistry
promoting
efficient
nano-aggregates
medicinal
plants
MAE,
provide
new
perspective
advancing
development
fusion
Chinese
herbal
medicine
nanomedicine
clinical
translation.
Язык: Английский
The Effects of Fumonisin B1 on Intercellular Communications and miRNA Modulations: Non-genotoxic Carcinogenesis Mechanisms in Human Kidney Cells
Toxicology,
Год журнала:
2024,
Номер
unknown, С. 153968 - 153968
Опубликована: Окт. 1, 2024
Язык: Английский
Resveratrol Alleviates Fumonisin B1-Induced Cytotoxicity in Sertoli Cells
Foods,
Год журнала:
2024,
Номер
13(23), С. 3810 - 3810
Опубликована: Ноя. 26, 2024
Fumonisin
B1
is
a
common
food
contaminant
that
has
been
found
to
adversely
affect
the
reproductive
system,
especially
Sertoli
cells.
However,
potential
mitigation
of
FB1-induced
cytotoxicity
in
cells
not
fully
elaborated.
Resveratrol
natural
substance
with
anti-inflammatory,
antioxidant,
and
anti-tumor
properties.
Herein,
protective
effects
resveratrol
against
were
examined
this
work.
The
mouse
cell
line
(TM4)
was
used
as
research
model.
These
results
indicated
FB1
(40
μM
80
μM)
significantly
reduces
viability,
disrupts
barrier,
induces
an
inflammatory
response
TM4
To
our
surprise,
(15
showed
ability
reverse
adverse
induced
by
μM).
Furthermore,
could
alleviate
apoptosis,
decrease
ROS
level,
promote
antioxidant
enzymes
(CAT
SOD2)
expression
FB1-treated
addition
mitigate
promoted
phosphorylation
JNK
upregulation
c-jun
expression.
Interestingly,
also
able
FB2
μM),
FB3
FB1-FB2-FB3
μM-40
combination
group
on
In
summary,
displayed
may
fumonisin
B1-induced
via
inhibiting
oxidative
stress-mediated
JNK/c-jun
signaling
pathway-induced
apoptosis.
This
study
provides
new
insights
into
prevention
treatment
testicular
toxicity
highlights
application
value
resveratrol.
Язык: Английский