Towards Understanding Particle-Protein Complexes: Physicochemical, Structural, and Cellbiological Characterization of β-Lactoglobulin Interactions with Silica, Polylactic Acid, and Polyethylene Terephthalate Nanoparticles
Colloids and Surfaces B Biointerfaces,
Год журнала:
2025,
Номер
253, С. 114702 - 114702
Опубликована: Апрель 22, 2025
Nanoplastic
particles
and
their
additives
are
increasingly
present
in
the
food
chain,
interacting
with
biomacromolecules
not
yet
known
consequences.
A
protein
corona
forms
around
these
usually
complex
matrices,
primarily
a
first
contact
at
surface-active
proteins.
However,
systematic
studies
on
interactions
between
proteins
-especially
regarding
affinity
structural
changes
due
to
surface
properties
like
polarity
-
limited.
It
is
also
unclear
whether
can
"mask"
particles,
mimic
properties,
induce
cytotoxic
effects
when
internalized
by
mammalian
cells.
This
study
aimed
investigating
physicochemical
of
model
particle-protein
complexes,
adsorbed
proteins,
Caco-2
Whey
β-lactoglobulin
(β-Lg)
was
used
as
well-characterized
studied
mixture
nanoparticles
varying
polarity,
specifically
silica,
polylactic
acid
(PLA),
polyethylene
terephthalate
(PET).
The
analyses
included
measurements
hydrodynamic
diameter
zeta
potential,
while
conformational
were
analyzed
using
Fourier-transform-infrared
spectroscopy
(FTIR)
intrinsic
fluorescence.
Cellular
uptake
cells
assessed
through
flow
cytometry,
cell
viability
measured
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide
(MTT)
assay,
cellular
impedance
xCELLigence®
technology.
results
indicated
that
β-Lg
had
highest
for
hydrophilic
silica
forming
silica-β-Lg
complexes
large
aggregates
electrostatic
interactions.
decreased
PLA
lowest
hydrophobic
PET,
which
formed
smaller
complexes.
Adsorption
onto
caused
partial
unfolding
refolding
β-Lg.
cells,
impairing
proliferation.
In
contrast,
PLA-
PET-protein
internalized,
though
slightly
reduced
viability.
enhances
our
understanding
adsorption
its
potential
biological
effects.
Язык: Английский
Interactions of Micro- and Nanoplastics with Biomolecules: From Public Health to Protein Corona Effect and Beyond
The Journal of Physical Chemistry B,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 25, 2025
Micro-
and
nanoplastics
(M/NPs),
as
ubiquitous
global
environmental
pollutants,
have
garnered
increasing
attention
due
to
their
pervasive
presence.
These
particles
can
interact
with
biological
molecules
through
various
mechanisms,
subsequently
inducing
potential
toxic
effects
on
living
organisms.
This
review
investigates
the
hazards
of
M/NPs
interactions
membranes
proteins,
focusing
interaction
mechanisms
biomolecular
structure
function.
Specifically,
we
summarize
exposure
pathways
harms
M/NPs,
which
enter
human
body
ingestion,
inhalation,
skin
contact,
potentially
causing
toxicity,
inflammation
responses,
oxidative
stress,
endocrine
disruption.
Additionally,
highlight
between
membranes,
induce
structural
changes,
including
membrane
thickening,
increased
fluidity,
pore
formation,
thereby
compromising
integrity
affecting
cellular
health.
Besides,
emphasize
suggesting
that
protein
changes
corona
formation
influence
stress
responses
cytotoxicity,
impacting
functions
viability.
Ultimately,
suggestions
outlooks
for
further
research
are
proposed.
Overall,
this
systematically
summarizes
current
biomolecules,
effects,
providing
researchers
a
comprehensive
understanding
field.
Язык: Английский
THE EFFECT OF NANOPLASTICS AND MICROPLASTICS ON LUNG MORPHOLOGY AND PHYSIOLOGY: A SYSTEMATIC REVIEW
The Medical and Ecological Problems,
Год журнала:
2024,
Номер
28(3), С. 42 - 60
Опубликована: Дек. 30, 2024
Airborne
microplastic
(NP)
and
nanoplastic
(MP)
pollution
has
emerged
as
a
pressing
environmental
concern
with
significant
implications
for
human
health.
While
MPs
are
present
both
indoors
outdoors,
indoor
concentrations
generally
higher
due
to
the
abrasion
of
household
materials,
furniture,
other
domestic
sources.
NPs,
when
inhaled,
accumulate
in
various
lung
regions,
exerting
toxic
effects
potentially
leading
respiratory
diseases.
This
systematic
review,
conducted
according
PRISMA
guidelines,
evaluates
recent
experimental
studies
on
pathogenic
impact
nanomicroplastics
(NMPs)
tissues
using
vivo
vitro
models.
The
review
included
10
analyzing
MP
accumulation
90
examining
pathogenetic
mechanisms
exposure.
Research
data
indicates
predominance
polymeric
fibers
such
polypropylene,
polyethylene
terephthalate,
polytetrafluoroethylene,
accounting
nearly
half
structures.
NMPs
exhibit
size-
composition-dependent
accumulation,
smaller
positively
charged
particles
showing
translocation
potential
systemic
circulation
organs.
highlights
synergistic
heavy
metals
pollutants,
interaction
pulmonary
surfactant,
role
exacerbating
Current
analysis
underscores
growing
interest
NMP-related
health
risks
identifies
knowledge
gaps,
including
need
standardization
NMP
toxicity
testing
further
exploration
biological
systems.
findings
emphasize
importance
mitigating
exposure
safeguard
pave
way
future
research
long-term
impacts
airborne
Язык: Английский