Optical (Cherenkov) dosimetry to unravel the radiobiological effects of radioisotope based therapy
European Journal of Nuclear Medicine and Molecular Imaging,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 22, 2025
Язык: Английский
Bridging high resolution sub-cellular imaging with physiologically relevant engineered tissues
SSRN Electronic Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
While
high-resolution
microscopic
techniques
are
crucial
for
studying
cellular
structures
in
cell
biology,
obtaining
such
images
from
thick
3D
engineered
tissues
remains
challenging.
In
this
review,
we
explore
advancements
fluorescence
microscopy,
alongside
the
use
of
various
fluorescent
probes
and
material
processing
to
address
these
challenges.
We
navigate
through
diverse
array
imaging
options
available
tissue
engineering
field,
wide
field
super-resolution
so
researchers
can
make
more
informed
decisions
based
on
specific
interest.
Finally,
provide
some
recent
examples
how
traditional
limitations
sub-cellular
architecture
within
have
been
overcome
by
combining
with
innovative
approaches.
Язык: Английский
3D visualization of neurovascular networks in pulp-exposed rat molars using tissue clearing techniques
Odontology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 25, 2025
Язык: Английский
Three-dimensional exploration of the chicken embryo, a comparative study of light sheet and histological visualisation
PLoS ONE,
Год журнала:
2025,
Номер
20(4), С. e0320483 - e0320483
Опубликована: Апрель 1, 2025
Ultramicroscopy
has
offered
new
avenues
into
the
visualisation
of
tissues
within
animal
models,
providing
three-dimensional
through
use
light
sheet
fluorescence
microscopy.
This
study
aimed
to
develop
and
apply
an
optical
tissue
clearing
method
investigate
application
microscopy
image
late-stage
chicken
embryos,
compare
anatomical
traditional
histological
staining.
Seventeen-day
old
specific
pathogen
free
embryos
were
collected,
fixed,
sectioned.
Haematoxylin
eosin
stained
sections
prepared
for
histology,
while
imaging
required
be
optically
clear.
For
this,
ethyl
cinnamate-based
was
utilised,
allowing
acquisition
clear,
unobstructed
images
significant
organ
structures
systems
using
only
autofluorescence.
The
established
techniques
provided
mapping
between
familiar
histology
images.
Rendering
organs
contextual
insights
surrounding
physiological
architecture
major
systems.
most
apparent
identification
pulmonary
vein
rendering
a
volumetric
projection
vasculature
branching
lung
subsequent
merging
left
side
heart.
Overall,
embryo
enhanced
by
combining
with
information
gained
Язык: Английский
Myelinated fiber labeling and orientation mapping of the human brain with light-sheet fluorescence microscopy
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
The
convoluted
network
of
myelinated
fibers
that
supports
behavior,
cognition,
and
sensory
processing
in
the
human
brain
is
source
its
extraordinary
complexity.
Advancements
tissue
optical
clearing,
3D
fluorescence
microscopy,
automated
image
analysis
have
enabled
unprecedented
insights
into
architecture
these
networks.
Here,
we
investigate
multiscale
organization
from
brainstem,
Broca's
area,
hippocampus,
primary
visual
cortex
by
exploiting
a
specific
fiber
staining
method,
light-sheet
microscopy
(LSFM),
an
advanced
spatial
orientation
tool.
Using
optimized
protocol
integrates
clearing
with
lipophilic
DiD
probe
to
achieve
uniform
deep
labeling,
generate
micrometerresolution
volumetric
reconstructions
multiple
regions
through
inverted
LSFM.
Automated
processing,
employing
unsupervised
Frangi
filters,
provides
distribution
functions
local
dispersion
maps.
This
enables
precise
characterization
directionality
white
matter
bundles,
linking
mesoscopic
structural
properties
details
computed
at
native
micrometric
resolution
LSFM
apparatus.
presented
workflow
illustrates
robust
platform
for
large-scale,
high-resolution
mapping,
which
may
facilitate
investigation
pathological
alterations
unparalleled
and,
furthermore,
validation
other
neuroimaging
modalities.
Язык: Английский
ClearAIM: dynamic quantitative label-free monitoring of tissue optical clearing
Kamil Kalinowski,
Anna Chwastowicz,
Piotr Arcab
и другие.
Advanced imaging.,
Год журнала:
2025,
Номер
2(2), С. 021003 - 021003
Опубликована: Янв. 1, 2025
Язык: Английский
Delivery and kinetics of immersion optical clearing agents in tissues: Optical imaging from ex vivo to in vivo
Tingting Yu,
Xiang Zhong,
Dongyu Li
и другие.
Advanced Drug Delivery Reviews,
Год журнала:
2024,
Номер
215, С. 115470 - 115470
Опубликована: Окт. 30, 2024
Язык: Английский
Machine learning methods for histopathological image analysis: Updates in 2024
Computational and Structural Biotechnology Journal,
Год журнала:
2024,
Номер
27, С. 383 - 400
Опубликована: Дек. 30, 2024
Язык: Английский
Bridging high resolution sub-cellular imaging with physiologically relevant engineered tissues
Frontiers in Mechanical Engineering,
Год журнала:
2024,
Номер
10
Опубликована: Ноя. 26, 2024
While
high-resolution
microscopic
techniques
are
crucial
for
studying
cellular
structures
in
cell
biology,
obtaining
such
images
from
thick
3D
engineered
tissues
remains
challenging.
In
this
review,
we
explore
advancements
fluorescence
microscopy,
alongside
the
use
of
various
fluorescent
probes
and
material
processing
to
address
these
challenges.
We
navigate
through
diverse
array
imaging
options
available
tissue
engineering
field,
wide
field
super-resolution
so
researchers
can
make
more
informed
decisions
based
on
specific
interest.
Finally,
provide
some
recent
examples
how
traditional
limitations
sub-cellular
architecture
within
have
been
overcome
by
combining
with
innovative
approaches.
Язык: Английский